Curcumin Inhibits Proliferation of Epstein–Barr Virus-Associated Human Nasopharyngeal Carcinoma Cells by Inhibiting EBV Nuclear Antigen 1 Expression

Liu, Limei; Yang, Jiaomin; Ji, Wuguang; Wang, Chao

doi:10.1155/2019/8592921

Cited by 16 publications

(19 citation statements)

References 43 publications

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“…The detailed method of Western blot assay was described in our previous publication [ 23 ]. Briefly, MCF7 and MDA-MB-468 cells were transfected with siRNA-SLC39A7 or the control siRNA for 48 h. Cells were rinsed, harvested and dissolved in RIPA lysis buffer (Beyotime Institute of Biotechnology, China) with 0.5% cocktail protease inhibitor (Roche Diagnostics).…”

Section: Methodsmentioning

confidence: 99%

Analysis of the prognostic significance of solute carrier (SLC) family 39 genes in breast cancer

2020

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Background: Breast cancer (BC) is the most common malignancy in females and remains a main cause of cancer-associated death worldwide. The solute carrier (SLC) groups of membrane transport proteins, which control the inﬂux of zinc, participate in ranging of physiological processes and may provide novel therapeutic targets of cancers. However, the prognostic values of individual SLC family 39 (SLC39A) genes in patients with BC are not clarified. Materials and Methods: The mRNA expression of SLC family 39 genes in BC was evaluated by using the UALCAN database. The prognostic values of overall survival (OS) of SLC family 39 genes in patients with BC were investigated by Kaplan-Meier plotter. The survival analysis of cells was determined by Project Achilles. Results: The analytic results suggested that SLC39A1, SLC39A3, SLC39A4, SLC39A5, SLC39A6, SLC39A7, SLC39A9, SLC39A10, SLC39A11 and SLC39A13 were significantly upregulated in BC tissues compared with normal breast tissues. However, SLC39A8 and SLC39A14 were expressed higher in normal tissues than in BC tissues. High expression of SLC39A2, SLC39A3, SLC39A4, SLC39A5, SLC39A7, SLC39A12 and SLC39A13 was significantly associated with worse OS in patients with BC. In contrast, high mRNA levels of SLC39A6 and SLC39A14 indicated favorable OS. Through subgroup analysis, all abnormal expressed SLC family members were correlated with prognoses of patients with specific BC. Moreover, SLC39A7 was associated with proliferation and cloning of BC. Conclusions: Our results suggested that SLC family 39 members were promising prognostic biomarkers of BC. The SLC39A7 played a key role in growth and survival of BC cells.

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Section: Methodsmentioning

confidence: 99%

Analysis of the prognostic significance of solute carrier (SLC) family 39 genes in breast cancer

2020

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“…The chemosensitizing effects of curcumin in EBV-positive cells may be explained by its capacity to modulate the EBV life cycle. Curcumin reduces levels of Epstein-Barr nuclear antigen 1, a critical protein for viral latency maintenance, promoting cell cycle arrest and apoptosis of EBV-nasopharyngeal carcinoma cells (46). Similarly, a previous study indicated that curcuminoids promote EBV lytic cycle reactivation, increasing the cell death of gastric and nasopharyngeal cell lines when combined with other lytic activators such as gemcitabine and valproic acid (15).…”

Section: Discussionmentioning

confidence: 92%

Curcumin sensitizes Epstein‑Barr‑immortalized lymphoblastoid cell lines to inorganic arsenic toxicity

Martínez-Castillo¹,

Cruz-Robledo²,

Hernández-Zavala³

et al. 2021

Exp Ther Med

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Chronic exposure to inorganic arsenic (iAs) through contaminated drinking water is an important health problem in certain countries. The use of phytochemicals such as curcumin has recently emerged as an alternative strategy for preventing cellular damage caused by iAs. The Epstein-Barr virus (EBV) affects ~90% of the population and experimental evidence suggested that curcumin mediates cytotoxicity against EBV-infected cells. Due to the potential for an interaction of these factors, the aim of the present study was to evaluate the effect of this phytochemical on iAs-related toxicity in EBV-infected cells. Two independent EBV-immortalized human lymphoblastoid cell lines (LCLs) were used as the model. The cell lines were first incubated with increasing concentrations of curcumin or iAs for 24 and 15 h, respectively, to determine the individual effects of each exposure on cell death. In the next experiment, cell cultures were pre-incubated with 5 µM curcumin for 9 h prior to treatment with 10 µM iAs for 15 h, followed by evaluation of cell death and the cell cycle profile via flow cytometry. The results indicated that individual treatment with either curcumin or iAs induced cell death in a concentration-dependent manner. Furthermore, curcumin pre-treatment enhanced iAs-induced cell death and promoted cell cycle arrest in G1 phase. Taken together, these results suggested that curcumin sensitizes EBV-positive LCLs to the cytotoxic effects of iAs.

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“…- Overexpression of Jab1 correlated with poor survival in NPC patients. Liu et al [ 43 ] HONE1 HK1 – Curcumin MG-132 Effect of curcumin on the proliferation, cycle arrest, and apoptosis of EBV + NPC cells Curcumin induced EBNA1 degradation via the proteasome-ubiquitin pathway Friboulet et al [ 44 ] C666–1 CNE2 (EBV-) 13 NPC biopsies C15 and C17 (EBV + in nude mice) NPC xenografts MG132 Epoxomicin Functions of c-IAP2 in NPC cells - RMT 5265 induces the proteasome-mediated degradation of c-IAP2, resulting from the enhanced polyubiquitination of c-IAP2 Hui et al [ 45 ] HONE1, HK1-EBV, HONE1-EBV, HA, C666–1 Female BALB/c nude (nu/nu) mice Bortezomib, SAHA (Vorinostat) Mechanisms of apoptosis and effects on lytic cycle activation of EBV - Combination of bortezomib and SAHA synergistically induce killing of a panel of NPC cell lines and suppresses the growth of NPC xenografts in nude mice. - Bortezomib inhibits SAHA’s induction of EBV replication and abrogates production of infectious viral particles in NPC cells.…”

Section: Introductionmentioning

confidence: 99%

“…In this sense, treatment of NPC cells with proteasome inhibitors resulted in a significant increase of total p27 levels. In parallel, Jab1/CSN5 overexpression happened to be inhibited, which may therefore indicate that Jab1/CSN5 promotes p27 degradation through the ubiquitin-dependent proteasome pathway which in turn may lead to cell-cycle progression [ 43 ].…”

Section: Introductionmentioning

confidence: 99%

Current approach and novel perspectives in nasopharyngeal carcinoma: the role of targeting proteasome dysregulation as a molecular landmark in nasopharyngeal cancer

Yarza

Bover

Agulló-Ortuño

et al. 2021

J Exp Clin Cancer Res

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Nasopharyngeal carcinoma (NPC) represents a molecularly paradigmatic tumor given the complex diversity of environmental as well as host dependent factors that are closely implicated in tissue transformation and carcinogenesis. Epstein Barr Virus (EBV) plays a key role in tissue invasion, hyperplasia and malignant transformation. Therefore, EBV related oncoviral proteins such as Latent Membrane Protein family (LMP1, LMP2), Epstein Barr Nuclear Antigen 1 (EBNA1) and EBV related glycoprotein B (gB) are responsible for inducing intracellular signalling aberrations leading to sustained proliferation and further acquisition of NPC related invasive nature and metastatic potential.Dysregulation of proteasome signaling seems to be centrally implicated in oncoviral protein stabilization as well as in modulating tumor microenvironment. Different studies in vitro and in vivo suggest a potential role of proteasome inhibitors in the therapeutic setting of NPC. Furthermore, alterations affecting proteasome signalling in NPC have been associated to tumor growth and invasion, distant metastasis, immune exclusion and resistance as well as to clinical poor prognosis. So on, recent studies have shown the efficacy of immunotherapy as a suitable therapeutic approach to NPC. Nevertheless, novel strategies seem to look for combinatorial regimens aiming to potentiate immune recognition as well as to restore both primary and acquired immune resistance.In this work, our goal is to thoroughly review the molecular implications of proteasome dysregulation in the molecular pathogenesis of NPC, together with their direct relationship with EBV related oncoviral proteins and their role in promoting immune evasion and resistance. We also aim to hypothesize about the feasibility of the use of proteasome inhibitors as part of immunotherapy-including combinatorial regimens for their potential role in reversing immune resistance and favouring tumor recognition and eventual tumor death.

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Curcumin Inhibits Proliferation of Epstein–Barr Virus-Associated Human Nasopharyngeal Carcinoma Cells by Inhibiting EBV Nuclear Antigen 1 Expression

Cited by 16 publications

References 43 publications

Analysis of the prognostic significance of solute carrier (SLC) family 39 genes in breast cancer

Analysis of the prognostic significance of solute carrier (SLC) family 39 genes in breast cancer

Curcumin sensitizes Epstein‑Barr‑immortalized lymphoblastoid cell lines to inorganic arsenic toxicity

Current approach and novel perspectives in nasopharyngeal carcinoma: the role of targeting proteasome dysregulation as a molecular landmark in nasopharyngeal cancer

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