Curcumin Inhibits the Growth and Induces Apoptosis of Human Endometrial Cancer Cells

Abstract

Li: Effect of Curcumin on Human Endometrial Cancer CellsThe objective of this study was to analyze the mechanism of curcumin inhibiting the growth and inducing apoptosis of human endometrial carcinoma cell line. Human endometrial carcinoma cell line was cultured in vitro and all cells were divided into control group, curcumin low, medium and high treatment group, the concentrations were 5 mg/l, 10 mg/l and 20 mg/l, respectively. Compared with the non-medicated control group, the proliferation of human endometrial carcinoma cell treated with curcumin at different concentrations for 24, 48 and 72 h was significantly inhibited and with the increase of drug concentration, the inhibition rate was significantly increased in a dose-dependent manner (p<0.05). After treatment of human endometrial carcinoma cell with curcumin 5 mg/l, 10 mg/l and 20 mg/l, the ratio of growth 0/ growth 1 was significantly lower than that of the control group, the ratio of growth 2/mitosis phase was significantly higher than that of the control group and the ratio of growth 2/mitosis phase was gradually increased with the increase of drug concentration, which was dose-dependent (p<0.05). After treatment of human endometrial carcinoma cells with curcumin 5 mg/l, 10 mg/l and 20 mg/l, the apoptosis rate gradually increased and with the increase of drug concentration, the apoptosis rate increased sequentially (p<0.05). After treatment of human endometrial carcinoma cell with curcumin 5 mg/l, 10 mg/l and 20 mg/l, compared with the control group, the protein level of apoptosis factor Livin was significantly decreased and the protein level of caspase-3 was significantly increased in each group (p<0.05). The levels of Livin protein and caspase-3 protein were dose-dependent with curcumin concentration. It was concluded that curcumin has obvious inhibitory effect on human endometrial cancer cells and can induce cell cycle arrest, promote apoptosis, which is concentration dependent. The related reason may be through inhibiting Livin protein expression and increasing caspase-3 protein level.

Ameliorating Effects of Curcumin on Testicular Cancer

“…One of these proposed therapeutic candidates for TC is curcumin, a polyphenol compound obtained from Curcuma longa, which is used as a colorant, flavoring ingredient, and cooking spice [22][23][24]. Curcumin has been demonstrated to have antineoplastic functions in many malignancies by some mechanisms, for instance, suppressing cell proliferation, inflammation, invasion, angiogenesis, and metastasis of tumor cells, stimulating apoptosis, and sensitizing cancer cells to cancer therapies [25][26][27][28][29]. Hereby, in this narrative review, we aimed to review evidence indicating the therapeutic capacity of curcumin for treating this cancer.…”