Curcumin inhibits the migration and invasion of human A549 lung cancer cells through the inhibition of matrix metalloproteinase-2 and -9 and Vascular Endothelial Growth Factor (VEGF)

Lin, Song; Lai, Kuang Chi; Hsu, Shu Chun; Yang, Jai Sing; Kuo, Chao Lin; Lin, Jing; Shih, Yi; Wu, Chih Chung; Chung, Jing Gung

doi:10.1016/j.canlet.2009.04.037

Cited by 225 publications

(182 citation statements)

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“…Matrigel Cell Migration Assay and Invasion System were used for examining cell migration and invasion as described previously (29)(30)(31). Briefly, the cell migration assay that was used with transwell (BD Biosciences, Franklin Lakes, NJ, USA) cell culture chambers (8 mm pore size; Millipore, Billerica, MA, USA) and coated with collagen.…”

Section: Chemicalsmentioning

confidence: 99%

Cantharidin Impairs Cell Migration and Invasion of Human Lung Cancer NCI-H460 Cells via UPA and MAPK Signaling Pathways

Hsia

Hsiao

et al. 2016

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Abstract. Cantharidin (CTD), a component of natural mylabris (Mylabris phalerata Pallas), has been shown to have biological activities and induce cell death in many human and the results indicated that CTD inhibited the enzymatic activities of MMP-2/-9 of NCI-H460 cells. Western blotting was used to examine the protein expression of NCI-H460 cells after incubation with CTD and the results showed that CTD decreased the expression of MMP-2/-9, focal adhesion kinase (FAK), Ras homolog gene family, member A (Rho A), phospho-protein kinase B (AKT) (Thr308)(p-AKT(308)), phospho-extracellular signal-regulated kinase1/2 (p-ERK1/2), phospho-p38 mitogen-activated protein (MAP) kinase (p-p38), phospho c-Jun N-terminal kinase 1/2 (p-JNK1/2), nuclear factor-ĸB (NF-ĸB) and urokinase plasminogen activator (UPA). Furthermore, confocal laser microscopy was used to confirm that CTD suppressed the expression of NF-ĸB p65, but did not significantly affect protein kinase C (PKC) translocation in NCI-H460 cells. Based on those observations, we suggest that CTD may be used as a novel anticancer metastasis agent for lung cancer in the future.Lung cancer is the most common cancer among women and men in Taiwan and it is the major cause of death in cancerrelated diseases in the developed world. Neoplastic metastasis is a major cause of cancer-mediated death in humans (1, 2). For tumor metastasis, epithelial cancer cells have to migrate from the original primary tumor mass via breaking their cellcell contacts (adherens junctions) to form cancer mass in a new site (3, 4); thus, tumor metastasis involves cell attachment (adhension), migration and invasion in order to form a new tumor in another site of the body. Inhibiting cancer cell metastasis is one of the important steps for cancer therapy and research (5). Numerous evidence have shown that multiple factors involved in tumor metastasis, such as the activation of PI3K/Akt pathway (6), matrix metalloproteinases (MMPs)-enzymes that can degrade the extracellular matrix and 5989

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Section: Chemicalsmentioning

confidence: 99%

Cantharidin Impairs Cell Migration and Invasion of Human Lung Cancer NCI-H460 Cells via UPA and MAPK Signaling Pathways

Hsia

Hsiao

et al. 2016

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“…Earlier studies indicated that curcumin can inhibit phospholipases, metalloproteinases and protein kinase C [35][36][37][38] that may act effectively against snake bite. In the present study, the efficacy of curcumin was increased by tagging with gold nanoparticle.…”

Section: Discussionmentioning

confidence: 99%

Russell´s viper (Daboia Russelli Russelli) Venom Toxicity Neutralizing Efficacy of Curcumin- Gold Nanoparticle (C-GNP) in Experimental Animal Model

2016

J Toxins

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“…Curcumin also has the ability to inhibit invasion and metastasis of cancer cells through regulating the expression of inflammatory cytokines, growth factors, growth factors' receptors, enzymes, adhesion molecules, apoptosis-related proteins and cell cycle proteins (10,(13)(14)(15)(16). Curcumin modulates the activity of oncogenes, tumor suppressor genes, several transcription factors and their signaling pathways (10,13,15,17).…”

Section: And Heat Shock Proteins (Hlj1) In Addition Possible Synergmentioning

confidence: 99%

“…Overexpression of MMPs observed in most of human cancer cells and high levels of MMPs are linked to metastasis (12). MMP-2 and -9 are the two main proteases that degrade the major component of basement membrane, type IV collagen, which, in turn, promote metastasis, especially in breast cancer (13,14). Therefore, MMP-2 and -9 are the key therapeutic targets for anti-metastatic agents.…”

Section: Curcumin Suppresses the Expression Of Matrix Metalloproteinamentioning

confidence: 99%

“…Curcumin has well-known biological activities that include anti-inflammatory, antioxidant, anti-microbial, wound healing and anti-cancer properties (6)(7)(8)(9)(10) to interfere with tumor cell cycle, thereby suppressing the growth of cancer cells and preventing invasion and metastasis (8,(10)(11)(12)(13). Curcumin also has the ability to inhibit invasion and metastasis of cancer cells through regulating the expression of inflammatory cytokines, growth factors, growth factors' receptors, enzymes, adhesion molecules, apoptosis-related proteins and cell cycle proteins (10,(13)(14)(15)(16). Curcumin modulates the activity of oncogenes, tumor suppressor genes, several transcription factors and their signaling pathways (10,13,15,17).…”

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Molecular Mechanisms of Anti-metastatic Activity of Curcumin

Deng

Verron

Rohanizadeh

2016

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Abstract. Cancer is the leading cause of death worldwide. Although cancer occurs as a localized disease, its morbidity and mortality rates remain high due to the ability of cancer cells to break-off from the primary tumor and spread to distant organs. Currently, chemotherapy is the main treatment for cancer; however, the increase in proportion of drug-resistant cancer cells and unpleasant side-effects of chemotherapy are still the major challenges in cancer therapy. Curcumin is a natural polyphenol compound and the main bioactive constituent of Indian spice turmeric, widely used in Indian and Chinese medicines. Curcumin has well-known therapeutic actions, including antiinflammatory, anti-microbial, anti-oxidant and anti-cancer properties. Curcumin induces cancer cell apoptosis through regulating various signaling pathways and arresting tumor cell cycle. Curcumin's therapeutic/ preventative actions on metastatic cancers have not been yet fully understood and studied. The present review explores the potential anti-metastatic mechanisms of curcumin, including inhibition of transcription factors and their signaling pathways (e.g., inflammatory cytokines (e.g., CXCL1, CXCL2, multiple proteases (e.g., uPA, MMPs), multiple protein kinases (e.g., MAPKs, FAK), regulation of miRNAs (e.g., miR21, miR181b) and heat shock proteins (HLJ1). In addition, possible synergistic actions of combination therapy of curcumin with current chemotherapies are discussed in this review.Despite all recent advances in oncology, cancer is still one of the deadliest diseases around the world (1). Cancer occurs as a localized disease but can spread to distant organs through migration, invasion and metastasis (2). Metastatic cancer is one of the major causes of death in cancer patients. Metastasis is a complex process involving multiple steps: (i) local migration through degrading basement membrane and extracellular matrix (ECM), (ii) intravasation into blood and/or lymphatic vessels, (iii) circulating to the target organ site, (iv) extravasation into target organ tissue and, finally, (v) multiplication in the target organ (2, 3). These steps are mediated by various factors, including growth factors, proteolysis degradation of extracellular matrix, cell-cell adhesion, cytoskeleton remodeling and changes of genes' expressions (2, 3). Metastasis is a non-random process that means each metastatic cancer type has its own preferred site of metastasis. For example, breast cancer cells preferentially metastasize to regional lymph nodes, liver, lungs and bone (4). Nowadays, there are different therapeutic approaches available for patients with metastatic cancers, including surgery, radiotherapy and chemotherapy. Chemotherapy remains the main treatment modality for cancer patients because of its ability of preventing invasion and metastasis. However, the morbidity and mortality rates in patients with metastatic cancer still remain high since current chemotherapy agents fail to selectively and effectively kill cancer cells without destroying normal ce...

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Curcumin inhibits the migration and invasion of human A549 lung cancer cells through the inhibition of matrix metalloproteinase-2 and -9 and Vascular Endothelial Growth Factor (VEGF)

Cited by 225 publications

References 40 publications

Cantharidin Impairs Cell Migration and Invasion of Human Lung Cancer NCI-H460 Cells via UPA and MAPK Signaling Pathways

Cantharidin Impairs Cell Migration and Invasion of Human Lung Cancer NCI-H460 Cells via UPA and MAPK Signaling Pathways

Russell´s viper (Daboia Russelli Russelli) Venom Toxicity Neutralizing Efficacy of Curcumin- Gold Nanoparticle (C-GNP) in Experimental Animal Model

Molecular Mechanisms of Anti-metastatic Activity of Curcumin

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