Curcumin-Loaded Self-Assembly Constructed by Octenylsuccinate Fish (Cyprinus carpio L.) Scale Gelatin: Preparation and Characterization

Abstract

Curcumin loaded octenylsuccinate fish scale gelatin (OFSG) was prepared in this study, to explore the potential of FSG for delivering hydrophobic nutrients. The effects of molecule weight (Mw, 22,677–369 g/mol) and degree of substitution (DS, 0–0.116) on the curcumin loading efficiency (CLE, μg/mL) of OFSG (6.98–26.85 mg/mL) were evaluated. The expose of interior hydrophobic groups in FSG and increased intermolecular hydrophobic area contributed to the loading of curcumin in two phases, respectively. The interaction between OFSG and curcumin showed a decreased absorption in FTIR and an increased crystallinity in XRD. The loading of curcumin into OFSG caused a significant decrease of the particle size (from 350–12,070 to 139–214 nm), PDI (from 0.584–0.659 to 0.248–0.347) and ζ-potential (−12.2 or −11.4 to −21.0 or −20.3). OFSG showed a significantly higher stability and lower release of curcumin than FSG at the end of the simulated gastrointestinal digestion. Thus, OFSG showed great potential in the construction of a carrier for hydrophobic nutrients.

Self-assembled nanoparticles of acid-induced fish (Cyprinus carpio L.) scale gelatin: Structure, physicochemical properties, and application for loading curcumin

“…This process was followed by the self-assembly of FSG into nanoparticles, with a hydrophobic layer formed in the center of the nanoparticles. The hydrophobic region collapsed to reduce hydrophobic domain interactions with water, thus the hydrophobic core was able to retain the hydrophobic curcumin molecules ( Yu et al, 2022 ). Subsequently, the curcumin was encapsulated within the assembled network.…”

“…The peak of FSG at 1649 cm −1 refers to amide I (1,600–1,700 cm −1 ) and derives from the stretching vibrations of the C O and C—N groups ( Sow & Yang, 2015 ). The peak of FSG at 1,554 cm −1 represents amide II and derives mainly from the C—N tensile vibration coupled with in-plane N—H bending modes, and C—C stretching vibrations ( Yu et al, 2022 ). The peak of FSG at 1,238 cm −1 refers to amide III and is mainly ascribed to C—N tensile vibration, N—H deformation of the amide bond, and the swinging vibrations of glycine and proline CH 2 groups.…”

“…Simulated gastric fluid (SGF) and intestinal fluid (SIF) were prepared following the detailed method provided by Yu et al (2022) . The concentration of the control sample (free curcumin) was measured to 21 μg/mL and dissolved in a solution containing 25 % dimethyl sulfoxide (DMSO).…”