Curcumin-Loaded Solid Lipid Nanoparticles Enhanced Anticancer Efficiency in Breast Cancer

Abstract

Curcumin (Cur) has been widely used in medicine, due to its antibacterial, anti-inflammatory, antioxidant, and antitumor effects. However, its clinic application is limited by its instability and poor solubility. In the present wok, curcumin was loaded into solid lipid nanoparticles (SLNs), in order to improve the therapeutic efficacy for breast cancer. The results measured using transmission electron microscopy (TEM) indicated that Cur-SLNs have a well-defined spherical shape; the size was about 40 nm with a negative surface charge. The drug loading and encapsulation efficiency in SLNs reached 23.38% and 72.47%, respectively. The Cur-SLNs showed a stronger cytotoxicity against SKBR3 cells. In vitro cellular uptake study demonstrated a high uptake efficiency of the Cur-SLNs by SKBR3 cells. Moreover, Cur-SLNs induced higher apoptosis in SKBR3 cells, compared to cells treated by free drug. In addition, Western blot analysis revealed that Cur-SLNs could promote the ratio of Bax/Bcl-2, but decreased the expression of cyclin D1 and CDK4. These results suggested that Cur-SLNs could be a potential useful chemotherapeutic formulation for breast cancer therapy.

Nanodelivery of Natural Antioxidants: An Anti-aging Perspective

“…These approaches, in particular, allow drug delivery directly to tumor sites without damaging nearby healthy tissues (Ahmad et al, 2018). In in vitro studies, enhanced anti-tumor activity was obtained, compared to respective unmodified substances, for nanoengineered phytobioactive compounds exerting antioxidant and anti-inflammatory activities such as resveratrol (Rodenak-Kladniew et al, 2017;Wang et al, 2017), curcumin (Meena et al, 2017;Dhivya et al, 2018;Montalban et al, 2018;Wang W. et al, 2018;Ni et al, 2019;Somu and Paul, 2019;van der Vlies et al, 2019), EGCG (Chavva et al, 2019), berberine (Wang et al, 2014a;Zheng et al, 2018), aloe-emodin (Wang et al, 2012;Chen et al, 2014), and oridonin (Wang et al, 2014b). In mouse models of the induced cancer, substantial inhibition of tumor proliferation and angiogenesis and also enhanced levels of apoptosis of cancerous cells have been found in animals administered, either orally or intravenously, with nanoparticles co-loaded with curcumin, along, or in combination with particular anti-cancer drugs (Wang et al, 2015b(Wang et al, , 2016Yang et al, 2015;Yan et al, 2016;Cui et al, 2017;Kumari et al, 2017;, in comparison to those for free substances.…”

<p>Targeted Therapy for Hepatocellular Carcinoma: Co-Delivery of Sorafenib and Curcumin Using Lactosylated pH-Responsive Nanoparticles</p>

“…35 Drug entrapment efficiency (EE) and loading capacity (LC) were analyzed using a UV spectrophotometer (Shimadzu, Kyoto, Japan) at 270 nm (for SFN) and 425 nm (for CCM). 36 The EE and LC were calculated by using equations:…”

Curcumin-Loaded Solid Lipid Nanoparticles Bypass P-Glycoprotein Mediated Doxorubicin Resistance in Triple Negative Breast Cancer Cells

“…The solid lipid matrix protects entrapped lipophilic drugs from chemical degradation and enhances their physical stability. SLN ameliorates the half-life of drugs in the systemic circulation, modulates their release kinetics, and increases the therapeutic efficacy of drugs used in anticancer therapy [20,21]. Moreover, SLN surfaces can be decorated by several agents.…”