Curcumin Nanoparticles: Preparation, Characterization, and Antimicrobial Study

Abstract

Curcumin is a highly potent, nontoxic, bioactive agent found in turmeric and has been known for centuries as a household remedy to many ailments. The only disadvantage that it suffers is of low aqueous solubility and poor bioavailability. The aim of the present study was to develop a method for the preparation of nanoparticles of curcumin with a view to improve its aqueous-phase solubility and examine the effect on its antimicrobial properties. Nanoparticles of curcumin (nanocurcumin) were prepared by a process based on a wet-milling technique and were found to have a narrow particle size distribution in the range of 2-40 nm. Unlike curcumin, nanocurcumin was found to be freely dispersible in water in the absence of any surfactants. The chemical structure of nanocurcumin was the same as that of curcumin, and there was no modification during nanoparticle preparation. A minimum inhibitory concentration of nanocurcumin was determined for a variety of bacterial and fungal strains and was compared to that of curcumin. It was found that the aqueous dispersion of nanocurcumin was much more effective than curcumin against Staphylococcus aureus , Bacillus subtilis , Escherichia coli , Pseudomonas aeruginosa , Penicillium notatum , and Aspergillus niger . The results demonstrated that the water solubility and antimicrobial activity of curcumin markedly improved by particle size reduction up to the nano range. For the selected microorganisms, the activity of nanocurcumin was more pronounced against Gram-positive bacteria than Gram-negative bacteria. Furthermore, its antibacterial activity was much better than antifungal activity. The mechanism of antibacterial action of curcumin nanoparticles was investigated by transmission electron micrograph (TEM) analysis, which revealed that these particles entered inside the bacterial cell by completely breaking the cell wall, leading to cell death.

Curcumin-loaded Chitosan Tripolyphosphate Nanoparticles as a safe,natural and effective antibiotic inhibits the infection of Staphylococcusaureus and Pseudomonas aeruginosa in vivo

“…Bhawana et al have also displayed that curcumin was able to inhibit the bacterial infection. They demonstrated that the cytotoxicity effect of curcumin-loaded chitosan-TPP nanoparticles was greater on Gram-positive bacteria than Gram-negative bacteria (29). Our experiments showed the efficacy and effectiveness of curcumin-loaded chitosan-TPP nanoparticles on both Gram-positive and Gram-negative bacteria including Staphylococcus aureus and Pseudomonas aeruginosa.…”

“…Curcumin as a potent, biocompatible and bioactive agent with an antimicrobial property has been applied in nanoparticles synthesis (28,29). In the present study, curcumin was loaded in chitosan-TPP nanoparticles by ionotropic gelation method.…”

Antibacterial Hydrogels

“…Allicin–CS complexes were proved to be active against bacteria involved in spoilage and can be used as an antibacterial agent in foods 96. Curcumin (CUR), a nontoxic and bioactive agent found in turmeric, has been applied for centuries as a remedy to various ailments 97. However, its applications were limited by its low aqueous solubility and poor bioavailability.…”

Characterization of Nanoparticles Produced by Chloroform Fraction of Kaempferia Rotunda Rhizome Loaded With Alginic Acid and Chitosan and Its Biological Activity Test

“…The results also showed that curcumin nanoparticles have better antibacterial activity. Curcumin nanoparticles are easier to get into the bacterial cell wall and can cause cell death [23]. The research of curcumin encapsulated that poly lactic-co-glycolic acid (PLGA) nanoparticles can destroy amyloid aggregates, exhibit antioxidative property, and are non-cytotoxic.…”