Curcumin on to hyaluronic acid conjugate enhance cytotoxicity

Abstract

Objective: Polymer-drug conjugates have gained much attention largely to circumvent lower drug solubility and to enhance drug stability. Curcumin is widely known for its medicinal properties including its anticancer efficacy. One of the serious drawbacks of curcumin is its poor water solubility which leads to reduced bioavailability. Materials and methods: Synthesized hyaluronic acid-curcumin (HA-Cur) conjugate. The drug conjugate was characterized using FT-IR technique. The conjugates, interestingly found to assembles as micelles in aqueous phase. The formationof micelles seems to improve the stability of the drug in physiological pH. We also assessed cytotoxicity of the conjugate usingMCF-7, HBL 100 and Hela cell line and quantified by MTT assay. The results showed Conjugate Results: (HA-Cur) produced extremely significant reduction in percentage cell viability in dose dependent inhibitory effect on growth of cell lines. Conjugated curcumin also showed cytotoxicity reflecting its potential in targeted Conclusion: drug delivery applications.

Microwave-Assisted, Solid-State Procedure to Covalently Conjugate Hyaluronic Acid to Curcumin: Validation of a Green Synthetic Protocol

“…The main hurdle of using CUR for treatment of diseases is its poor solubility and lack of stability in aqueous medium, in particular in basic–neutral conditions, − so that several attempts have been made to enhance its bioavailability in the bloodstream. For instance, CUR has been encapsulated in the inner core of carriers, such as chitosan, hyaluronan, poly­(lactic- co -glycol acid), and so on, − and chemically conjugated to natural polysaccharides in order to develop amphiphilic nanocarriers of CUR. − Hyaluronic acid (HA), as a hydrosoluble polysaccharide, increases the solubility and bioavailability of CUR, while providing its own additional biological benefits (antimicrobial, anti-inflammatory, antioxidant, wound-repairing effects). − …”