Curcumin, Polydatin and Quercetin Synergistic Activity Protects from High-Glucose-Induced Inflammation and Oxidative Stress

Abstract

Chronic hyperglycemia, the diagnostic biomarker of Type 2 Diabetes Mellitus (T2DM), is a condition that fosters oxidative stress and proinflammatory signals, both involved in the promotion of cellular senescence. Senescent cells acquire a proinflammatory secretory phenotype, called SASP, exacerbating and perpetuating the detrimental effects of hyperglycemia. Bioactive compounds can exert antioxidant and anti-inflammatory properties. However, the synergistic anti-inflammatory and antioxidant effects of the most extensively investigated natural compounds have not been confirmed yet in senescent cells and in hyperglycemic conditions. Here, we exposed young and replicative senescent HUVEC (yHUVEC and sHUVEC) to a high-glucose (HG) condition (45 mM) and treated them with Polydatin (POL), Curcumin (CUR) and Quercetin (QRC), alone or in combination (MIX), to mirror the anti-inflammatory component OxiDefTM contained in the novel nutraceutical GlicefenTM (Mivell, Italy). In both yHUVEC and sHUVEC, the MIX significantly decreased the expression levels of inflammatory markers, such as MCP-1, IL-1β and IL-8, and ROS production. Importantly, in sHUVEC, a synergistic effect of the MIX was observed, suggesting its senomorphic activity. Moreover, the MIX was able to reduce the expression level of RAGE, a receptor involved in the activation of proinflammatory signaling. Overall, our data suggest that the consumption of nutraceuticals containing different natural compounds could be an adjuvant supplement to counteract proinflammatory and pro-oxidative signals induced by both hyperglycemic and senescence conditions.

Lipid lowering and anti-ageing effects of edible flowers of Viola x wittrockiana Gams in a Caenorhabditis elegans obese model

“…76,77 Quercetin, one of the most representative polyphenols, has proven to have a role in age-related diseases, exhibits neuroprotective activity, prevents diabetes, protects cells from highglucose induced inflammation and increases C. elegans lifespan. 20,78,79 One of the mechanisms in which this fat reduction might be happening is through a diminution in the ingestion rate as we can observe in both the E. coli and pumping assays. The ingestion of fluorescent bacteria was increased under the obese conditions, except for the one supplemented with the extract.…”

“…76,77 Quercetin, one of the most representative polyphenols, has proven to have a role in age-related diseases, exhibits neuroprotective activity, prevents diabetes, protects cells from high-glucose induced inflammation and increases C. elegans lifespan. 20,78,79…”

Zingiber officinale Roscoe Rhizome Extract Exerts Senomorphic and Anti-Inflammatory Activities on Human Endothelial Cells

“…The primary human endothelial cells obtained from the umbilical vein (HUVEC) were extensively investigated as a human model of endothelial cells to assess the effects of senolytic and senomorphic compounds [ 26 , 27 , 28 , 29 ]. We observed that 10 µg/mL of ZOE exerts anti-inflammatory activity on LPS-stimulated yHUVEC by reducing the expression levels of IL-1β, IL-8, MCP-1, TNF-α, and ICAM-1.…”

Polydatin improves vascular endothelial function by maintaining mitochondrial homeostasis under high glucose conditions

“…Poly was dissolved in 0.5% dimethysulfoxide (DMSO) and directly diluted in the medium to a concentration of 10 µM for further experiments. The concentration of Poly was determined based on the literatures 50 , 51 and our preliminary experiment (Supplementary Figs. 4 and 5 ).…”