Curcumin prevents diabetic nephropathy against inflammatory response via reversing caveolin-1 Tyr14 phosphorylation influenced TLR4 activation

Sun, Lina; Yang, Zhao; Lv, Shunzeng; Liu, Xiangchun; Guan, Guangju; Liu, Gang

doi:10.1016/j.intimp.2014.08.023

Cited by 50 publications

(42 citation statements)

References 45 publications

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“…Renal ischemia plays a major role in the development of diabetic polyneuropathy (Nukada, 2014). Curcumin ameliorated diabetic polyneuropathy via reversing caveolin-1 Tyr14 phosphorylation and subsequent suppression of TLR4 activation and inflammation (Sun et al, 2014). has been shown in many tumor types, suggesting the role of TLR4 signaling in the development and progression of cancer (Kelly et al, 2006).…”

Section: Renal Ischemiamentioning

confidence: 99%

Impact of curcumin on toll‐like receptors

Boozari

Butler²,

Sahebkar

2019

Journal Cellular Physiology

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Toll‐like receptors (TLRs) have a pivotal role in the activation of innate immune response and inflammation. TLRs can be divided into two subgroups including extracellular TLRs that recognize microbial membrane components (TLR1, 2, 4, 5, 6, and 10), and intracellular TLRs that recognize microbial nucleic acids (TLR3, 7, 8, and 9). Curcumin is a dietary polyphenol from Curcuma longa L. that is reputed to have diverse biological and pharmacological effects. Extensive research has defined the molecular mechanisms through which curcumin mediates its therapeutic effects. One newly defined and important target of curcumin is the TLR, where it exerts an inhibitory effect. In the current study, we focus upon the TLR antagonistic effect of curcumin and curcumin's therapeutic effect as mediated via TLR inhibition. The available evidence indicates that curcumin inhibits the extracellular TLR 2 and 4 and intracellular TLR9 and thereby exerts a therapeutic effect in diseases such as cancer, inflammation, infection, autoimmune, and ischemic disease. Curcumin effectively modulates the TLR response and thereby exerts its potent therapeutic effects.

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Section: Renal Ischemiamentioning

confidence: 99%

Impact of curcumin on toll‐like receptors

Boozari

Butler²,

Sahebkar

2019

Journal Cellular Physiology

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“…Sun et al reported that curcumin could prevent EMT of podocytes by suppressing the phosphorylation of Cav-1 and increasing stabilization of Cav-1 and β -catenin [173]. In addition, curcumin could inhibit renal inflammatory gene expression in vivo by reducing Cav-1 phosphorylation at Tyr (14) [174]. Yuan et al investigated the mechanism of curcumin in increasing Cav-1 expression and found that curcumin increased the expression of Cav-1 by inhibiting nuclear translocation of SREBP-1 [175].…”

Section: Cav-1 and Oxidative Stress Regulationmentioning

confidence: 99%

Caveolin‐1: An Oxidative Stress‐Related Target for Cancer Prevention

Wang

Zheng

et al. 2017

Oxidative Medicine and Cellular Longevity

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Aberrant oxidative metabolism is one of the hallmarks of cancer. Reactive species overproduction could promote carcinogenesis via inducing genetic mutations and activating oncogenic pathways, and thus, antioxidant therapy was considered as an important strategy for cancer prevention and treatment. Caveolin-1 (Cav-1), a constituent protein of caveolae, has been shown to mediate tumorigenesis and progression through oxidative stress modulation recently. Reactive species could modulate the expression, degradation, posttranslational modifications, and membrane trafficking of Cav-1, while Cav-1-targeted treatments could scavenge the reactive species. More importantly, emerging evidences have indicated that multiple antioxidants could exert antitumor activities in cancer cells and protective activities in normal cells by modulating the Cav-1 pathway. Altogether, these findings indicate that Cav-1 may be a promising oxidative stress-related target for cancer antioxidant prevention. Elucidating the underlying interaction mechanisms between oxidative stress and Cav-1 is helpful for enhancing the preventive effects of antioxidants on cancer, for improving clinical outcomes of antioxidant-related therapeutics in cancer patients, and for developing Cav-1 targeted drugs. Herein, we summarize the available evidence of the roles of Cav-1 and oxidative stress in tumorigenesis and development and shed novel light on designing strategies for cancer prevention or treatment by utilizing the interaction mode between Cav-1 and oxidative stress.

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“…Cell lysates and kidney cortex homogenate were rapidly lysed for protein isolation and analyzed as previously described [18] .…”

Section: Western Blot Assaymentioning

confidence: 99%

Curcumin attenuates high glucose-induced podocyte apoptosis by regulating functional connections between caveolin-1 phosphorylation and ROS

et al. 2016

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Aim: Caveolin-1 (cav-1) is a major multifunctional scaffolding protein of caveolae. Cav-1 is primarily expressed in mesangial cells, renal proximal tubule cells and podocytes in kidneys. Recent evidence shows that the functional connections between cav-1 and ROS play a key role in many diseases. In this study we investigated whether regulating the functional connections between cav-1 and ROS in kidneys contributed to the beneficial effects of curcumin in treating diabetic nephropathy in vitro and in vivo. Methods: Cultured mouse podocytes (mpc5) were incubated in a high glucose (HG, 30 mmol/L) medium for 24, 48 or 72 h. Male rats were injected with STZ (60 mg/kg, ip) to induce diabetes. ROS generation, SOD activity, MDA content and caspase-3 activity in the cultured cells and kidney cortex homogenate were determined. Apoptotic proteins and cav-1 phosphorylation were analyzed using Western blot analyses. Results: Incubation in HG-containing medium time-dependently increased ROS production, oxidative stress, apoptosis, and cav-1 phosphorylation in podocytes. Pretreatment with curcumin (1, 5, and 10 μmol/L) dose-dependently attenuated these abnormalities in HG-treated podocytes. Furthermore, in HG-containing medium, the podocytes transfected with a recombinant plasmid GFP-cav-1 Y14F (mutation at a cav-1 phosphorylation site) exhibited significantly decreased ROS production and apoptosis compared with the cells transfected with empty vector. In diabetic rats, administration of curcumin (100 or 200 mg/kg body weight per day, ig, for 8 weeks) not only significantly improved the renal function, but also suppressed ROS levels, oxidative stress, apoptosis and cav-1 phosphorylation in the kidneys. Conclusion: Curcumin attenuates high glucose-induced podocyte apoptosis in vitro and diabetic nephropathy in vivo partly through regulating the functional connections between cav-1 phosphorylation and ROS.

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Curcumin prevents diabetic nephropathy against inflammatory response via reversing caveolin-1 Tyr14 phosphorylation influenced TLR4 activation

Cited by 50 publications

References 45 publications

Impact of curcumin on toll‐like receptors

Impact of curcumin on toll‐like receptors

Caveolin‐1: An Oxidative Stress‐Related Target for Cancer Prevention

Curcumin attenuates high glucose-induced podocyte apoptosis by regulating functional connections between caveolin-1 phosphorylation and ROS

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