Curcumin Promotes KLF5 Proteasome Degradation through Downregulating YAP/TAZ in Bladder Cancer Cells

Gao, Yang; Shi, Qi; Xu, Shan; Du, Chong; Liang, Liang; Wu, Kaijie; Wang, Ke; Wang, Xinyang; Chang, Luke S.; He, Dalin; Guo, Peng

doi:10.3390/ijms150915173

Cited by 71 publications

(74 citation statements)

References 32 publications

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“…It is the main curcuminoid found in the spice turmeric, a plant alkaloid obtained from ground rhizome of the perennial herb Curcuma longa (13). According to investigations, curcumin has multiple biofunctional activities including anti-inflammatory, antioxidant, anti-cancer, anti-diabetes and chemo preventative activities (14)(15)(16)(17)(18).…”

Section: Nakhostinmentioning

confidence: 99%

The Effect of Curcumin Supplementation on Selected Markers of Delayed Onset Muscle Soreness (DOMS)

Nakhostin-Roohi

Moradlou

Hamidabad

et al. 2016

Ann. Appl. Sport Sci.

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Inflammation and pain induced by delayed onset muscle soreness (DOMS) can be induced by eccentric exercise or an unaccustomed activity. The condition can causes problems in exercising and for athletes. The purpose of this study was to assess the effect of 150mg curcumin supplementation immediately after intensive eccentric exercise. Evaluations were made for total antioxidant Capacity (TAC), muscle damage markers, and DOMS induced pain. Ten healthy young males (age, 25.0 ± 1.6 years; height, 178.9 ± 4.1 cm; body mass, 81.1 ± 6.8 kg; fat%, 14.2 ± 2.1) completed a double blind randomized-controlled crossover trial to estimate the effects of oral curcumin supplementation (150mg) and a placebo on squat performance and DOMS following unaccustomed heavy eccentric exercise. Curcumin (CU) or placebo (P) was taken at the prescribed dose immediately after eccentric squat exercises; administrations were separated by a 14-day washout period. Measurements were made at the baseline, immediately, 24, 48, and 72h after exercise comprising: (a) limb pain (1-10 cm visual analogue scale; VAS), (b) total antioxidant capacity (TAC) (c) serum markers of muscle damage and inflammation. Measurements taken after exercise showed significantly reduced levels of pain, creatine kinase (CK), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in C group compared with group P group (P<0.05). TAC remained significantly high in group C after exercise compared with levels in group P (P<0.05). The findings of this study suggest that a 150mg dose of curcumin may have antioxidant, anti-inflammatory and analgesic effects on DOMS.

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Section: Nakhostinmentioning

confidence: 99%

The Effect of Curcumin Supplementation on Selected Markers of Delayed Onset Muscle Soreness (DOMS)

Nakhostin-Roohi

Moradlou

Hamidabad

et al. 2016

Ann. Appl. Sport Sci.

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“…It has been reported that KLF5 has a role in different cell processes, including in cell proliferation, cell cycle progression and apoptosis (9)(10)(11). Previous studies have demonstrated that KLF5 acts as an oncogene in different cancer cells, and is a potential tumor suppressor in prostate cancer cells (24).…”

Section: Discussionmentioning

confidence: 99%

“…Western blot analysis was performed using a previously described method (11). A total of 2x10 6 LNCaP cells were washed once with cold PBS and lysed in radioimmunoprecipitation assay buffer (50 mM Tris pH 8.0, 150 mM NaCl, 0.1% SDS, 1% NP-40, and 0.5% sodium deoxycholate) containing 1% protease inhibitor cocktail and 1 mM phenylmethanesulfonyl fluoride (Sigma-Aldrich; Merck KGaA).…”

Section: Methodsmentioning

confidence: 99%

“…RT-qPCR was performed using a previously described method (11). Briefly, total RNA from 2x10 5 LNCaP cells was isolated using TRIzol reagent (Thermo Fisher Scientific, Inc.) and reverse transcribed into cDNA using the PrimeScript™ RT reagent kit (Takara Biotechnology Co., Ltd., Dalian, China).…”

Section: Reverse Transcription-quantitative Pcr (Rt-qpcr)mentioning

confidence: 99%

“…The Krüppel-like transcription factor 5 (KLF5/IKLF/ BTEB2), a type of zinc-finger transcription factor, has important roles in various cellular processes, including in cell proliferation, cell cycle progression and apoptosis (9)(10)(11). Several previous studies have demonstrated that KLF5 acts as an oncogene in different types of cancer, including in bladder and breast cancer (10,12).…”

Section: Introductionmentioning

confidence: 99%

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KLF5 promotes apoptosis induced by phorbol ester as an effector of the autocrine factor TNFα in LNCaP prostate cancer cells

et al. 2017

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Abstract. Krüppel-like factor 5 (KLF5) is frequently deleted and inactivated in prostate cancer, and exerts tumor-suppressing function in prostate cancer cells. However, the function of KLF5 in the apoptosis of prostate cancer cells remains unclear. In the present study, the effect of KLF5 on phorbol 12-myristate 13-acetate (PMA)-induced apoptosis was investigated in prostate cancer LNCaP cells. It was demonstrated that PMA induced the expression of KLF5 at the mRNA and protein level. To identify whether KLF5 regulates the activity of the downstream pathway, stable KLF5 knockdown or overexpression cell lines were constructed with lentivirus harboring shRNA targeting KLF5 or full-length KLF5 in LNCaP cells. Knockdown of KLF5 significantly decreased PMA-induced apoptosis, while cell apoptosis was significantly increased following KLF5 overexpression compared with the corresponding control groups. Consistently, expression of cleaved poly(ADP-ribose) polymerase and caspase-3 induced by PMA was decreased following KLF5 knockdown and increased following KLF5 overexpression. Using the control medium from cells treated with PMA, it was demonstrated that KLF5 is required for the control medium to induce apoptosis. c-Jun N-terminal kinase (JNK) activity is essential for the apoptosis induced by PMA. It was revealed that knockdown of KLF5 decreased, while overexpression of KLF5 increased the phosphorylation of JNK induced by PMA and control medium treatment. Furthermore, inhibition of tumor necrosis factor α (TNFα) decreased KLF5 expression and significantly decreased cell apoptosis induced by PMA, and control medium. This data indicates that KLF5 is essential for the apoptosis induced by PMA in LNCaP prostate cancer cells. Furthermore, KLF5 is essential for activity of the autocrine factor TNFα, which is secreted by cells treated with PMA and mediates the function of PMA-induced apoptosis through regulating the activity of JNK signaling pathway. These results provide novel insights into the complexity of the signaling pathways regulating apoptosis in prostate cancer cells, which could aid in the development of novel treatments for patients with prostate cancer.

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KLF5 promotes cell migration by up‐regulating FYN in bladder cancer cells

Gao

et al. 2016

FEBS Letters

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Kr€ uppel-like factor 5 (KLF5) promotes cell proliferation of bladder cancer. However, whether KLF5 regulates other cell processes in bladder cancer is not clear. We found that KLF5 increases cell migration and lamellipodia formation, expression of FYN and phosphorylation of FAK in bladder cancer cells. In addition, KLF5 promotes transcription of FYN through binding to its promoter. FYN overexpression rescues cell migration and lamellipodia formation reduced by KLF5 knockdown. Furthermore, the KLF5/FYN/p-FAK axis is necessary for lysophosphatidic acid (LPA) to promote cell migration. Our findings indicate that both KLF5 and FYN are important in the regulation of cell migration in bladder cancer cells. We propose the KLF5/FYN/p-FAK axis as a potential therapeutic target in bladder cancer.

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Curcumin Promotes KLF5 Proteasome Degradation through Downregulating YAP/TAZ in Bladder Cancer Cells

Cited by 71 publications

References 32 publications

The Effect of Curcumin Supplementation on Selected Markers of Delayed Onset Muscle Soreness (DOMS)

The Effect of Curcumin Supplementation on Selected Markers of Delayed Onset Muscle Soreness (DOMS)

KLF5 promotes apoptosis induced by phorbol ester as an effector of the autocrine factor TNFα in LNCaP prostate cancer cells

KLF5 promotes cell migration by up‐regulating FYN in bladder cancer cells

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