Curcumin reduces inflammation in rat apical periodontitis

Abstract

Aim The aim of the study was to evaluate the effect of systemic curcumin administration on the severity of apical periodontitis (AP). Methodology Forty male Wistar rats weighing 250–280 g each, age 2.5 months, were distributed into four groups (n = 10): control untreated rats (C), control rats treated with curcumin (CUR), rats with pulp exposure‐induced apical periodontitis (AP) and rats with pulp exposure‐induced apical periodontitis treated with curcumin (AP‐CUR). Curcumin treatment was administered orally once daily for 15 days before pulp exposure and continued for 30 days after pulp exposure. The rats were sacrificed at 30 days, and the jaws were collected and reconstructed in a programme specific for micro‐CT. The jaws were processed for analysis of the inflammatory process using haematoxylin and eosin staining and immunohistochemical assays for interleukin tumour necrosis factor alpha (TNF‐α), interleukin (Il)‐6 and Il‐1β. Tartrate‐resistant acid phosphatase (TRAP) and osteocalcin (OCN) staining were used to analyse the resorptive process on the bone surface of periapical area. Kruskal–Wallis with Dunn's test was performed for nonparametric data and anova with Tukey's test for parametric data, p < .05. Results Micro‐CT revealed no statistically significant differences in bone resorption between the AP and AP‐CUR groups (p > .05). The levels of inflammatory cell infiltration and immunoreactivity for the proinflammatory cytokines TNF‐α, Il‐6 and Il‐1β were significantly higher in the periapical lesions of the AP group than in the AP‐CUR group (p < .05). The number of TRAP‐positive multinucleated cells was higher in the AP group than in the AP‐CUR group (p < .05). In OCN‐positive cells, no differences were observed between the AP and AP‐CUR groups (p > .05). Conclusions Oral supplementation with curcumin had a significant effect on the AP severity in rats, suggesting an anti‐inflammatory effect of curcumin on AP development.

Curcumin Attenuates Periodontal Injury via Inhibiting Ferroptosis of Ligature-Induced Periodontitis in Mice

“…Curcumin is a potent antioxidant that facilitates mitochondrial activities, reduces the level of ROS and lipid peroxidation, improves SOD activity and inhibits erastin-induced ferroptosis [ 40 , 41 ]. Many studies have shown that curcumin has a potential role in the treatment of periodontitis [ 42 , 43 ]. However, the exact mechanism of curcumin in ligature-induced periodontitis has not been studied clearly.…”

Systemic inhibition of 5-lipoxygenase by MK-886 exacerbates apical periodontitis bone loss in a mouse model

“…In this study, a histological and histometric evaluation was performed to understand the signaling pathways and mediators involved in bone resorption. This method is commonly used to determine periapical bone loss in induced apical periodontitis [ 10 , 12 ] but this analysis could be improved through tomographic volumetric measurements [ 36 – 38 ]. This is one limitation of the present study, which should be better explored in future studies.…”