Curcumin reverses glomerular hemodynamic alterations and oxidant stress in 5/6 nephrectomized rats

Tapia, Edilia; Zatarain-Barrón, Zyanya Lucía; Hernández-Pando, Rogelio; Zarco-Márquez, Guillermo; Molina‐Jijón, Eduardo; Cristóbal-García, Magdalena; Santamaría, José; Pedraza‐Chaverrí, José

doi:10.1016/j.phymed.2012.11.014

Cited by 78 publications

(69 citation statements)

References 14 publications

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“…Previous studies have showed that some antioxidant compounds, such as N-acetylcysteine, melatonin, S-allylcysteine, vitamin E and curcumin are potential candidate in inhibiting renal dysfunction [20,[29][30][31][32]. In the present study, as expected, HQD decreased the expression of NADPH oxidase significantly, so HQD may exert efficacy through suppressing oxidative stress.…”

Section: Discussionsupporting

confidence: 82%

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Improvement of Huangqi Decoction on Endothelial Dysfunction in 5/6 Nephrectomized Rats

Chu

Wang

Mao

et al. 2016

Cell Physiol Biochem

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Background/Aims: Endothelial dysfunction is a major factor in the progression of chronic kidney disease, which correlates with oxidative stress and NO deficiency. Huangqi decoction (HQD) is a potential anti-oxidant ingredient in renoprotection. However, the underlying mechanisms remained identified. Therefore, we investigated whether HQD exhibit improvement in endothelial dysfunction in the 5/6 nephrectomy (Nx) rat model. Methods: Male Wistar rats (180 - 250 g) were divided into sham, Nx and Nx + HQD (0.05, 0.15 and 0.45 g/kg) group, respectively. Renal function and histology were examined with ELISA and Immunohistochemical analysis. Endothelium-dependent relaxation of rat aortas was investigated by isometric tension recordings. Oxidative stress and NO bioavailability were detected by ELISA, DHE-staining, DAF-2 staining and western blotting. Results: Compared with Nx rats, HQD treatment reversed the functional and structural changes of kidney significantly. Besides, endothelium-dependent relaxation of rat aortas was also improved by HQD treatment. NADPH oxidase and ROS generation were inhibited while NO bioavailability was enhanced. Conclusion: HQD can act as a potent prescription for the treatment of endothelium related vascular complications.

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Section: Discussionsupporting

confidence: 82%

“…Paraffin sections (4 μm) were stained with 0.5% periodic acid and Schiff (PAS) and Masson. Semiquantitative scoring of glomerular sclerosis in PAS-stained slides and interstitial collagen deposition in Masson-stained slides were performed using method described previously [20].…”

Section: Histopathological Analysismentioning

confidence: 99%

Improvement of Huangqi Decoction on Endothelial Dysfunction in 5/6 Nephrectomized Rats

Chu

Wang

Mao

et al. 2016

Cell Physiol Biochem

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“…Therefore, SKG may improve renal damage by reducing the serum lipid concentration. Furthermore, CRF leads to a decreased glomerular filtration rate and the accumulation of Scr, BUN, proteinuria and other metabolites, resulting in an increase in the total oxygen free radical content (24,25). GSH-PX is an important enzyme that eliminates free radicals (26,27).…”

Section: Discussionmentioning

confidence: 99%

Effect of Shenkang granules on the progression of chronic renal failure in 5/6 nephrectomized rats

Zhang

Zhou

Wang

et al. 2015

Experimental and Therapeutic Medicine

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Abstract. Shenkang granules (SKGs) are a Chinese herbal medicinal formula, consisting of rhubarb (Rheum palmatum L.), Salvia miltiorrhiza, milkvetch root [Astragalus membranaceus (Fisch.) Bunge] and safflower (Carthamus tinctorius L.). The aim of the present study was to investigate the effect of SKG on chronic renal failure (CRF) in 5/6 nephrectomized (5/6 Nx) rats. The rats were randomly divided into seven groups (n=10 per group) as follows: (i) 5/6 Nx (model group; 2.25 ml/kg/day normal saline); (ii) SKGL (low dose; 5/6 Nx treated with 2 g crude drug/kg/day SKG); (iii) SKGM (moderate dose; 5/6 Nx treated with 4 g crude drug/kg/day SKG); (iv) SKGH (high dose; 5/6 Nx treated with 8 g crude drug/kg/day SKG); (v) benazepril treatment group (5/6 Nx treated with 5 mg/kg/day benazepril); (vi) Shenkang injection (SKI) group (5/6 Nx with 13.3 ml/kg/day SKI); and (vii) sham-operated group (2.25 ml/kg/day normal saline). After 30 days, the levels of microalbumin, total protein, serum creatinine, blood urea nitrogen and serum lipid were found to be significantly decreased in the SKGL and SKGM rats, showing histological improvement compared with the untreated 5/6 Nx rats, as determined by hematoxylin and eosin, and Masson's trichrome staining. In addition, SKG was found to significantly improve the levels of glutathione peroxidase and reduce the damage caused by free radicals to the kidney tissues. Furthermore, SKG prevented the accumulation of extracellular matrix by decreasing the expression of collagen I and III and inhibiting the expression of matrix metalloproteinases-2 and -9 in the renal tissue, as determined by western blot analysis. SKG was also shown to decrease the concentrations of serum transforming growth factor-β 1 , as determined by ELISA, and kidney angiotensin II, as determined using a radioimmunoassay kit. In conclusion, SKG was demonstrated to ameliorate renal injury in a 5/6 Nx rat model of CRF. Thus, SKG may exert a good therapeutic effect on CRF.

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“…Clinical study also showed that the accumulation of AGEs might contribute to the increase of OS in renal tissue which then leads to DN. OS has been regarded as one of the mechanisms on renal structural and functional alterations, for example, interstitial fibrosis, fibrotic glomeruli, tubular atrophy, and mesangial expansion [31]. Thus, the increase of antioxidant capacity might be one of the important events in the protection of oxidative stress for renal injury.…”

Section: Discussionmentioning

confidence: 99%

The Attenuation of Moutan Cortex on Oxidative Stress for Renal Injury in AGEs-Induced Mesangial Cell Dysfunction and Streptozotocin-Induced Diabetic Nephropathy Rats

Zhang

Feng

et al. 2014

Oxidative Medicine and Cellular Longevity

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Oxidative stress (OS) has been regarded as one of the major pathogeneses of diabetic nephropathy (DN) through damaging kidney which is associated with renal cells dysfunction. The aim of this study was to investigate whether Moutan Cortex (MC) could protect kidney function against oxidative stress in vitro or in vivo. The compounds in MC extract were analyzed by HPLC-ESI-MS. High-glucose-fat diet and STZ (30 mg kg−1) were used to induce DN rats model, while 200 μg mL−1 AGEs were for HBZY-1 mesangial cell damage. The treatment with MC could significantly increase the activity of SOD, glutathione peroxidase (GSH-PX), and catalase (CAT). However, lipid peroxidation malondialdehyde (MDA) was reduced markedly in vitro or in vivo. Furthermore, MC decreased markedly the levels of blood glucose, serum creatinine, and urine protein in DN rats. Immunohistochemical assay showed that MC downregulated significantly transforming growth factor beta 2 (TGF-β2) protein expression in renal tissue. Our data provided evidence to support this fact that MC attenuated OS in AGEs-induced mesangial cell dysfunction and also in high-glucose-fat diet and STZ-induced DN rats.

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Curcumin reverses glomerular hemodynamic alterations and oxidant stress in 5/6 nephrectomized rats

Cited by 78 publications

References 14 publications

Improvement of Huangqi Decoction on Endothelial Dysfunction in 5/6 Nephrectomized Rats

Improvement of Huangqi Decoction on Endothelial Dysfunction in 5/6 Nephrectomized Rats

Effect of Shenkang granules on the progression of chronic renal failure in 5/6 nephrectomized rats

The Attenuation of Moutan Cortex on Oxidative Stress for Renal Injury in AGEs-Induced Mesangial Cell Dysfunction and Streptozotocin-Induced Diabetic Nephropathy Rats

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