Curcuminoids Suppress the Growth and Induce Apoptosis through Caspase-3-Dependent Pathways in Glioblastoma Multiforme (GBM) 8401 Cells

Abstract

Curcuminoids, natural plant components, have been recently shown to display antioxidant and anti-inflammatory activities. They also produce potent chemo-preventive action against several types of cancer. In the present study, the anti-proliferative and induced apoptosis effects of curcuminoids have been investigated in human brain glioblastoma multiforme (GBM) 8401 cells. Results indicated that curcuminoids have produced an inhibition of cell proliferation in a dose-dependent manner as dosage increased from 12.5 to 100 μM (n = 6) via the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay as well as activation of apoptosis in GBM 8401 cells. Both effects were observed to increase in proportion with the dose of curcuminoids. We have studied the mitochondrial membrane potential (ΔΨm), DNA fragmentation, caspase-3, caspase-8, and caspase-9 activation, and nuclear factor κB (NF-κB) transcriptional factor activity to analyze apoptosis in GBM 8401 cells. From these approaches, apoptosis was induced by curcuminoids in human brain GBM 8401 cells via mitochondria and a caspase-dependent pathway. The results observed with proliferation inhibition (y = 94.694e(-0.025x), R(2) = 0.9901, and n = 6) and apoptosis (y = 0.9789e(-0.0102x), R(2) = 0.99854, and n = 3) depend upon the amount of curcuminoid treatment in the cancer cells.

Antioxidant and antitumor effects of polysaccharides from the fungus Pleurotus abalonus

“…It is clear that the increased proliferation and decreased cell death (apoptosis) are two major processes that contribute to the progression of tumor cell growth [33]. LDH is a stable cytoplasmic enzyme present in all cells and is rapidly released into the surrounding medium when membrane of cells is damaged [34]. For this reason, LDH assay is widely used as an indicator of irreversible cell death due to cellular membrane damage of various molecules [34].…”

The functional curcumin liposomes induce apoptosis in C6 glioblastoma cells and C6 glioblastoma stem cells in vitro and in animals

“…[46][47][48] Previous studies found 49,50 that the inhibitory mechanism of p53 that relied on NF-κB existed in the kidney cancer cells. Quinacrine inhibited NF-κB and activated tumor suppressors of p53 simultaneously.…”

Expression of pituitary tumor-transforming 2 in human glioblastoma cell lines and its role in glioblastoma tumorigenesis

“…In addition, caspase-3, a widely accepted hallmark of programmed cell death, is able to cleave a broad range of cellular substrates and promote activation of DNA endonuclease (23); thus the activation of caspases-3 is a key molecular stage in apoptotic cell death in glioblastoma multiforme. Huang et al (24) demonstrated that curcuminoids can also suppress cell growth and induce apoptosis in GBM 8401 cells through affecting caspase-3-dependent signaling pathways. In the present study, flow cytometry results demonstrated that the number apoptotic cells in the PTTG2 overexpression group were significantly reduced in comparison with the untreated group, while the number of apoptotic cells in the siRNA-PTTG2 interference group were reduced in comparison with the untreated group.…”