Curcumol inhibits the proliferation of gastric adenocarcinoma MGC-803 cells via downregulation of IDH1

Abstract

Curcumol, a polyphenol compound derived from the rhizome of Curcuma, has been established as an antitumor compound against multiple types of cancer, including gastric (GC), lung, liver and breast cancer. However, the molecular mechanisms undelying its anticancer activity in GC are still unclear. In this study, the antitumor efficacy of curcumol was ascertained in human gastric adenocarcinoma MGC-803 cells. An MTT assay was used to assess the viability of the MGC-803 cells treated by curcumol. The results of the Annexin V/propidium iodide (PI) staining followed by fluorescence activated cell sorting (FACS) analysis demonstrated that the cell cycle was arrested in the G2/M phase by curcumol. Annexin V-FITC/PI double staining followed by FACS analysis revealed that curcumol induced apoptosis of MGC-803 cells. FACS analysis after the cells were loaded with a DFCH-DA probe revealed that the level of reactive oxygen species (ROS) increased after the cells were treated with curcumol. In adittion, FACS analysis after the cells were loaded with JC-1 revealed that the level of mitochondrial membrane potential (MMP) decreased after the cells were treated with curcumol. Furthermore, the downregulation of isocitrate dehydrogenase 1 (IDH1) was observed in the MGC-803 cells after being treated with curcumol as determined by western blotting and RT-qPCR. In conclusion, we elucidated the antitumor effect of curcumol on MGC-803 cells and the involved mechanisms related to the induction of apoptosis, the increase of ROS, the decrease of MMP and the downregulation of IDH1.

Integration of medicinal plants into the traditional system of medicine for the treatment of cancer in Sokoto State, Nigeria

“…(2017) ; Mou et al. (2017) ; Wang et al., 2017 , Wang et al., 2017 ; Zang et al. (2017) ; Zhou et al.…”

Investigation on structural, magneto-transport, magnetic and thermal properties of La0.8Ca0.2−xBaxMnO3 (0⩽x⩽0.2) manganites

“…In particular La 1-x Ca x MnO 3 (LCMO) compounds have attracted extra attention because of occurrence of enormous magneto-resistance (MR) and huge magneto-caloric effect (MCE) which makes them good candidates for applications [5][6][7][8][9][10][11][12][13][14][15].…”

Curcumol: From Plant Roots to Cancer Roots

“…Curcumol has been turned up as unique paradigm for the multitargeted prevention of cancer. Anticancer potential of curcumol has been declared to be linked with apoptosis induction via p53 regulation [55], accumulation of ROS/oxidative stress [68], reducing antiapoptotic proteins (Bcl-2, Bcl-xl) [27,64], increasing proapoptotic proteins (Bax, Bad) [64], modulation of MAPK pathway (inhibition of p-JNK1/2, upregulation of p-p38 MAPK) [56,64], inhibition of NF-κB [64], triggering PARP cleavage [27], activation of caspase cascade, and diminishing mitochondrial membrane potential [52] (Figure 3).…”