Curdlan oligosaccharides having higher immunostimulatory activity than curdlan in mice treated with cyclophosphamide

Tang, Jiqing; Zhen, Hongmin; Wang, Nannan; Yan, Qiaojuan; Hao, Jing; Jiang, Zhengqiang

doi:10.1016/j.carbpol.2018.10.120

Cited by 50 publications

(22 citation statements)

References 57 publications

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“…Curdlan oligosaccharides from Alcaligenes faecalis var. myxogenes activate MAPK and NF-κB pathways and improve immunostimulatory activity in CY-induced imuunosuppressed mice [46]. Polysaccharides of Lonicera japonica , a typical Chinese herbal medicine, and wheat bran-derived polysaccharides markedly promote the serum cytokine levels [47,48], and polysaccharides derived from Stichopus japonicus (sea cucumber), up-regulate the expression of cytokines, such as IL-1β, IL-4, IL-6, IL-10, TNF-α, and IFN-γ, in the CY-induced mice [49].…”

Section: Resultsmentioning

confidence: 99%

In Vivo and In Vitro Study of Immunostimulation by Leuconostoc lactis-Produced Gluco-Oligosaccharides

Lee

Song

2019

Molecules

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Glycosyltransferase-producing Leuconostoc lactis CCK940 produces CCK- oligosaccharides, gluco-oligosaccharide molecules, using sucrose and maltose as donor and acceptor molecules, respectively. In this study, the immunostimulatory activities of CCK-oligosaccharides on RAW264.7 macrophages and BALB/c mice were evaluated. CCK-oligosaccharides induced the expression of phosphorylated-p38, extracellular-signal-regulated kinase (ERK), and c-Jun N-terminal kinase (JNK) and upregulation of phagocytic activity in RAW264.7 macrophages, suggesting their involvement in mitogen-activated protein kinase (MAPK) signaling pathway and phagocytosis. When CCK-oligosaccharides were administered to mice intraperitoneally injected with cyclophosphamide (CY), spleen indices and expressions of interleukin (IL)-6, IL–10, and tumor necrosis factor-α increased, compared with those in only CY-treated group. These findings suggest that CCK-oligosaccharides can be used as an effective immunostimulating agent.

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Section: Resultsmentioning

confidence: 99%

In Vivo and In Vitro Study of Immunostimulation by Leuconostoc lactis-Produced Gluco-Oligosaccharides

Lee

Song

2019

Molecules

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“…They are resistant to digestion throughout the gastrointestinal tract and show prebiotic effects to improve the growth of Lactobacillus strains [30]. Recently, GOS were reported to regulate the immune responses in mice treated with cyclophosphamide [32]. In the present study, we evaluated the effects of GOS on macrophage polarization and the underlying molecular mechanisms were investigated.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, TLR2 and CR3 (CD11b/CD18) on BMDMs could both recognize GOS and active the immune responses. However, for the RAW264.7 cells, GOS have been reported to interact with the receptors of TLR2 and CD18 to activate the macrophages, but independent with that of CD11b [32], while both CR3 (CD11b/CD18) and TLR2 were responsible for the recognition of linear β-glucan from the baker’s yeast to activate RAW264.7 cells [44].…”

Section: Discussionmentioning

confidence: 99%

“…myxogenes ) induced cytokines production of splenocytes and bone marrow-derived dendritic cells [31]. As compared with curdlan, GOS (DP 2−5) prepared by enzymatic hydrolysis using GH family 64 β-(1→3)-glucanase ( Rm Lam81A) showed superior immunostimulatory effects in cyclophosphamide induced immunosuppressed mice [32]. Similar improvement on immunostimulatory effects have been reported on paramylon derived (1→3)-β- d -glucan oligosaccharides using exo-β-1, 3-glucanase.…”

Section: Introductionmentioning

confidence: 99%

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Curdlan (Alcaligenes faecalis) (1→3)-β-d-Glucan Oligosaccharides Drive M1 Phenotype Polarization in Murine Bone Marrow-Derived Macrophages via Activation of MAPKs and NF-κB Pathways

Tang

et al. 2019

Molecules

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Functional oligosaccharides, particularly curdlan (1→3)-β-d-glucan oligosaccharides (GOS), play important roles in modulating host immune responses. However, the molecular mechanisms underlying the immunostimulatory effects of GOS on macrophage polarization are not clear. In this work, GOS (5–1000 µg/mL) were non-toxic to bone marrow-derived macrophages (BMDMs) with improved pinocytic and bactericidal capacities. Incubation with GOS (100 µg/mL) induced M1 phenotype polarization of BMDMs as evidenced by increased CD11c+/CD86+ (10.1%) and M1 gene expression of inducible nitric oxide synthase, interleukin (IL)-1β, and chemokine C-C-motif ligand 2. Accordingly, the secretion of cytokines IL-1β, IL-6, monocyte chemotactic protein-1, and tumor necrosis factor-α, as well as the nitrite release of BMDMs were increased by GOS (100 µg/mL). Expression of mitogen-activated protein kinases (MAPKs) of phosphorylated (p)-c-Jun amino-terminal kinase, p-extracellular signal regulated kinase, and p-p38 in BMDMs were increased by GOS, as well as the p-Stat1. Moreover, nuclear factor-kappa B (NF-κB) p-p65 expression in BMDMs was promoted by GOS while it suppressed IκBα expression. Receptor blocking with anti-CR3 (CD11b/CD18) and anti-toll-like receptor (TLR) 2 antibodies diminished GOS induced M1 phenotype polarization with reduced mRNA expression of M1 genes, decreased cytokine and nitrite releases, and suppressed signaling pathway activation. Thus, CR3 (CD11b/CD18) and TLR2 mediated activation of MAPKs and NF-κB pathways are responsible for GOS induced polarization of BMDMs.

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“…It has been used as an immobilization carrier in chemical engineering, construction and other fields [28,29]. It has also been used as a biologically active substance in the medical field [30,31] with antitumor, antibacterial, drug delivery and other functions. Furthermore, it has been used as stabilizers, gels, and quality improvers in food industry, particularly in biomimetic food, meat products and rice and flour products [32,33]…”

Section: Introductionmentioning

confidence: 99%

A Study of the Synergistic Interaction of Konjac Glucomannan/Curdlan Blend Systems under Alkaline Conditions

Yan

Pang

et al. 2019

Materials

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To improve the gelation performance of konjac glucomannan (KGM) thermo-irreversible gel in the condition of alkaline, this study investigated the interactions between KGM and curdlan (CUD) in terms of the sol state and gelation process. The apparent viscosity, rheological properties during heating and cooling, thermodynamic properties, gelation properties and water holding capacity of KGM/CUD blend systems in an alkaline environment were studied using physical property testing instruments and methods. The results showed that the viscosity of the KGM/CUD blended solution was greater than the value calculated from the ideal mixing rules in the condition of alkaline (pH = 10.58). As the proportion of CUD in the system increased, the intersection of storage modulus (G’) and loss modulus (G”) shifted to low frequencies, the relaxation time gradually increased, and the degree of entanglement of molecular chains between these two components gradually increased. The addition of CUD helped decrease the gelation temperature of KGM, increased the gelation rate and inhibited the thinning phenomenon of KGM gels at low temperatures (2–20 °C). The addition of CUD increased the hardness and gel strength of KGM but did not significantly improve the water holding capacity of the KGM/CUD blend gel. The process of mixing KGM and CUD improved the thermal stability of the gel. In summary, KGM/CUD exhibited excellent compatibility under alkaline conditions, and the blend systems produced a “viscosifying effect”. KC8 and KC5 show better thermal stability, low temperature resistance and gel strength compared to KGM. This blended gel can be used as a structural support material to provide reference for the development of konjac bionic vegetarian products.

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Curdlan oligosaccharides having higher immunostimulatory activity than curdlan in mice treated with cyclophosphamide

Cited by 50 publications

References 57 publications

In Vivo and In Vitro Study of Immunostimulation by Leuconostoc lactis-Produced Gluco-Oligosaccharides

In Vivo and In Vitro Study of Immunostimulation by Leuconostoc lactis-Produced Gluco-Oligosaccharides

Curdlan (Alcaligenes faecalis) (1→3)-β-d-Glucan Oligosaccharides Drive M1 Phenotype Polarization in Murine Bone Marrow-Derived Macrophages via Activation of MAPKs and NF-κB Pathways

A Study of the Synergistic Interaction of Konjac Glucomannan/Curdlan Blend Systems under Alkaline Conditions

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