Curli Produced by
            <i>Escherichia coli</i>
            PHL628 Provide Protection from Hg(II)

Hidalgo, Gabriela; Chen, Xincai; Lion, Leonard W.

doi:10.1128/aem.01254-10

Cited by 27 publications

(29 citation statements)

References 19 publications

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“…The content of salts modifies osmolarity, which increases the activity of the MerR promoter (Condee & Summers, 1992) and also modulates the biological availability of mercury (Barkays, 1997). This last might be related to the formation of cell clusters (Hidalgo et al, 2010) or changes in the charges of mercury ions (Golding et al, 2008). The bioluminescence of E. coli ARL1 induced with HgCl 2 (2.5 g L −1 ) increased with an increasing concentration of NaCl up to 250 mmol L −1 (Fig.…”

Section: Induction Of Bioluminescence With Mercury Chloride In Mediummentioning

confidence: 96%

Whole-cell optical biosensor for mercury – operational conditions in saline water

2015

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The present study demonstrates the influences of chlorides, fluorides and bromides of potassium and sodium on the growth and Hg 2+ -induced bioluminescence of bioreporter Escherichia coli ARL1. In a Luria-Bertani medium (LB), cell growth was inhibited by concentrations of sodium and potassium fluorides above 0.2 mol L −1 . The addition of NaCl increased cell tolerance to the toxic effects of fluorides and bromides. Lag periods of 10 h and more were observed for cultivations in LB without NaCl and with halides (NaCl, KCl, NaBr, KBr, NaF and KF) at concentrations lower than 0.06 mol L −1 . In a phosphate buffer (PB), the bioluminescence of E. coli ARL1, induced with HgCl2, was increased by the addition of NaCl, KCl, NaBr, KBr, NaF and KF (concentration of 0-0.25 mol L −1 ). In a saline phosphate buffer (PBS), the maxima of induced bioluminescence declined to 50 %, in the case of NaF (0.12 mol L −1 ), and to zero for KF. An addition of tryptone to the induction medium increased induced light emission ten-fold. Concentrated artificial sea water (ASW) (70-100 % ASW) inhibited bioluminescence induction. The new detection assay with E. coli ARL1 made possible the detection of 0.57 µg L −1 of HgCl2 in double-diluted artificial sea water (25 % ASW).

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Section: Induction Of Bioluminescence With Mercury Chloride In Mediummentioning

confidence: 96%

Whole-cell optical biosensor for mercury – operational conditions in saline water

2015

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“…Of the mechanisms known to confer Hg resistance to microorganisms (Barkay et al, 2003;Hidalgo et al, 2010;Latinwo et al, 1998) the MerA-mediated system is the most successful Hg detoxification known to date. Phylogenetic reconstructions strongly support an origin and early evolution of MerA, the central function of the mer operons, in geothermal environments (Barkay et al, 2010) and subsequent developments by localization on plasmids, addition of transport functions, and increasing sophistication of regulatory circuits that tightly control mer operon expression (Boyd and Barkay, 2012).…”

Section: Relating Mera Distribution and Frequency In Hot Spring Micromentioning

confidence: 99%

Microbes in mercury-enriched geothermal springs in western North America

Geesey

Barkay

King

2016

Science of The Total Environment

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Because geothermal environments contain mercury (Hg) from natural sources, microorganisms that evolved in these systems have likely adapted to this element. Knowledge of the interactions between microorganisms and Hg in geothermal systems may assist in understanding the long-term evolution of microbial adaptation to Hg with relevance to other environments where Hg is introduced from anthropogenic sources. A number of microbiological studies with supporting geochemistry have been conducted in geothermal systems across western North America. Approximately 1 in 5 study sites include measurements of Hg. Of all prokaryotic taxa reported across sites with microbiological and accompanying physicochemical data, 42% have been detected at sites in which Hg was measured. Genes specifying Hg reduction and detoxification by microorganisms were detected in a number of hot springs across the region. Archaeal-like sequences, representing two crenarchaeal orders and one order each of the Euryarchaeota and Thaumarchaeota, dominated in metagenomes' MerA (the mercuric reductase protein) inventories, while bacterial homologs were mostly found in one deeply sequenced metagenome. MerA homologs were more frequently found in metagenomes of microbial communities in acidic springs than in circumneutral or high pH geothermal systems, possibly reflecting higher bioavailability of Hg under acidic conditions. MerA homologs were found in hot springs prokaryotic isolates affiliated with Bacteria and Archaea taxa. Acidic sites with high Hg concentrations contain more of Archaea than Bacteria taxa, while the reverse appears to be the case in circumneutral and high pH sites with high Hg concentrations. However, MerA was detected in only a small fraction of the Archaea and Bacteria taxa inhabiting sites containing Hg. Nevertheless, the presence of MerA homologs and their distribution patterns in systems, in which Hg has yet to be measured, demonstrates the potential for detoxification by Hg reduction in these geothermal systems, particularly the low pH springs that are dominated by Archaea.

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“…The biofilms of E. coli are partly composed of a functional amyloid nanofibre, curli, which plays a role in bacterial adhesion 44 , aggregation 43 and biofilm stability 37,45 . Our results show that the curli system in E. coli is capable of secreting a wide variety of chimeric CsgA-peptide constructs that can self-assemble into the ECM as amyloid nanofibres.…”

Section: Discussionmentioning

confidence: 99%

Programmable biofilm-based materials from engineered curli nanofibres

et al. 2014

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The significant role of biofilms in pathogenicity has spurred research into preventing their formation and promoting their disruption, resulting in overlooked opportunities to develop biofilms as a synthetic biological platform for self-assembling functional materials. Here we present Biofilm-Integrated Nanofiber Display (BIND) as a strategy for the molecular programming of the bacterial extracellular matrix material by genetically appending peptide domains to the amyloid protein CsgA, the dominant proteinaceous component in Escherichia coli biofilms. These engineered CsgA fusion proteins are successfully secreted and extracellularly self-assemble into amyloid nanofibre networks that retain the functions of the displayed peptide domains. We show the use of BIND to confer diverse artificial functions to the biofilm matrix, such as nanoparticle biotemplating, substrate adhesion, covalent immobilization of proteins or a combination thereof. BIND is a versatile nanobiotechnological platform for developing robust materials with programmable functions, demonstrating the potential of utilizing biofilms as large-scale designable biomaterials.

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Curli Produced by Escherichia coli PHL628 Provide Protection from Hg(II)

Cited by 27 publications

References 19 publications

Whole-cell optical biosensor for mercury – operational conditions in saline water

Whole-cell optical biosensor for mercury – operational conditions in saline water

Microbes in mercury-enriched geothermal springs in western North America

Programmable biofilm-based materials from engineered curli nanofibres

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