Curli Production and Genetic Relationships Among Escherichia coli from Cases of Bovine Mastitis

Dyer, J.G.; Sriranganathan, N.; Nickerson, S.C.; Elvinger, François

doi:10.3168/jds.s0022-0302(07)72620-6

Cited by 10 publications

(6 citation statements)

References 29 publications

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“…The genes encoding curli fimbriae were present in all isolates, which indicates that differences in phenotype are the result of differences in gene expression, as reported by others (Dyer et al, 2007). Expression of csgD is highly regulated (reviewed by Van Houdt and Michiels, 2005).…”

Section: Discussionsupporting

confidence: 62%

Genomic and phenotypic characterization of Escherichia coli isolates recovered from the uterus of puerperal dairy cows

Silva¹,

Leitão²,

Tenreiro

et al. 2009

Journal of Dairy Science

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The role of Escherichia coli in the pathogenesis of the puerperal uterine infection of the cow is largely unknown. It is proposed that E. coli favors the persistence of Arcanobacterium pyogenes and gram-negative bacteria that are pivotal to the establishment of the infection. Here, we report the genomic and phenotypic characteristics of 72 E. coli isolates recovered from the uterus of dairy cows with normal puerperium (n = 12; 35 isolates) or clinical metritis (n = 18; 37 isolates), in an attempt to identify characteristics that are related to the establishment of uterine infection. We evaluated DNA fingerprints generated by repetitive element sequence-based PCR, phylogenetic grouping, the presence of 15 virulence factor genes, in vitro biofilm formation and its relationship to curli fimbriae expression, and cellulose production. We found a wide genetic diversity (40 clonal types), including types common to normal puerperium and clinical metritis cows (n = 6), as well as types specific to normal puerperium (n = 14) or clinical metritis (n = 20) cows. Isolates were assigned to phylogenetic groups B1 (58%), A (31%), and D (11%). Only 4 virulence factor genes were detected (hlyE, hlyA, iuc, and eaeA). In vitro biofilm formation was significantly affected by culture medium and incubation temperature. Curli fimbriae expression and cellulose production, although related to biofilm formation, were not required for it. None of the evaluated E. coli characteristics were significantly related to the establishment of the uterine infection. In conclusion, data presented in this paper indicate that E. coli isolates recovered from the uterus of puerperal cows present a wide genetic diversity, do not belong to a known pathogenic group, and have a low potential of virulence and persistence. This corroborates the putative role of the bacterium in the pathogenesis of the puerperal uterine infection of the cow.

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Section: Discussionsupporting

confidence: 62%

Genomic and phenotypic characterization of Escherichia coli isolates recovered from the uterus of puerperal dairy cows

Silva¹,

Leitão²,

Tenreiro

et al. 2009

Journal of Dairy Science

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“…Genome plasticity is the main driver of E. coli phenotypic diversity and evolution [48]; thus, this dynamism is responsible for the relevant differences observed among the pathogenic strains associated with diseases [49]. In the present study, the genomic diversity among vaginal E. coli was assessed through the ERIC-PCR technique as previously applied for animal samples taken from different mucosae [50,51].…”

Section: Discussionmentioning

confidence: 99%

Characterization of native Escherichia coli populations from bovine vagina of healthy heifers and cows with postpartum uterine disease

et al. 2020

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Even though Escherichia coli are common bacteria of the bovine vaginal microbiota, they represent an important pathogen that causes diseases in the reproductive tract and subfertility. However, the actual endometrial virulence profile of E. coli is poorly understood. The present study aims to characterize the phylogenetic structure and virulence potential of native vaginal populations of E. coli from healthy heifers (H), and cows with postpartum uterine diseases (PUD), such as metritis/endometritis (MT) or repeat breeder cows (RB). To this end, the virulence repertoire of 97 E. coli isolates was genotypically and phenotypically assessed. Most of them were assigned to phylogenetic group A (74%), followed by B1 (17%) and D (9%); RB strains were significantly (p < 0.05) more represented by B1. Seven of the 15 evaluated virulence genes (VFG) were detected and the most prevalent were fimH (87%), agn43 (41%) and csgA (35%); while traT (27%), fyuA (11%), hlyA (5%) and kpsMT II (5%) were observed in a lower proportion. Particularly, fyuA was significantly higher (p < 0.05) in MT cows whereas csgA showed the same behavior in PUD animals (p < 0.05). When comparing H and PUD strains, these last ones were associated to positive expression of biofilm, fimbriae curli/cellulose and motility; yet RB strains did not show motility. Vaginal B1 E. coli populations, that possess VFG (fyuA and csgA) as well as the expression of motility, curli fimbriae/cellulose and biofilm, may represent risk factors for endometrial disorders; specifically, those that also, have kpsMT II may have a pathogenic potential for causing the RB syndrome. Future research focusing on the detection of these strains in the vaginal microbiota of cows with postpartum uterine diseases should be done since the control of their presence in vagina could reduce the risk that they access the uterus during the postpartum period.

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“…Clinical signs in acute coliform mastitis are induced by LPS and the subsequent release of inflammatory mediators (Lohuis et al, 1988;Burvenich et al, 2003;Kornalijnslijper et al, 2004). No specific virulence determinants have been detected in E. coli isolated in mastitis (Lehtolainen et al, 2003;Wenz et al, 2006a;Dyer et al, 2007;Suojala et al, 2011).…”

Section: Characteristics Of Escherichia Coli Mastitismentioning

confidence: 99%

Treatment for bovineEscherichia colimastitis – an evidence‐based approach

Suojala

Kaartinen

Pyörälä

2013

Vet Pharm & Therapeutics

139

110

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Bovine mastitis caused by Escherichia coli can range from being a subclinical infection of the mammary gland to a severe systemic disease. Cow-dependent factors such as lactation stage and age affect the severity of coliform mastitis. Evidence for the efficacy of antimicrobial treatment for E. coli mastitis is very limited. Antimicrobial resistance is generally not a limiting factor for treatment, but it should be monitored to detect changes in resistance profiles. The only antimicrobials for which there is some scientific evidence of beneficial effects in the treatment for E. coli mastitis are fluoroquinolones and cephalosporins. Both are critically important drugs, the use of which in animals destined for food should be limited to specific indications and should be based on bacteriological diagnosis. The suggested routine protocol in dairy herds could target the primary antimicrobial treatment for mastitis, specifically infections caused by gram-positive bacteria. In E. coli mastitis with mild to moderate clinical signs, a non-antimicrobial approach (anti-inflammatory treatment, frequent milking and fluid therapy) should be the first option. In cases of severe E. coli mastitis, parenteral administration of fluoroquinolones, or third- or fourth-generation cephalosporins, is recommended due to the risk of unlimited growth of bacteria in the mammary gland and ensuing bacteremia. Evidence for the efficacy of intramammary-administered antimicrobial treatment for E. coli mastitis is so limited that it cannot be recommended. Nonsteroidal anti-inflammatory drugs have documented the efficacy in the treatment for E. coli mastitis and are recommended for supportive treatment for clinical mastitis.

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Curli Production and Genetic Relationships Among Escherichia coli from Cases of Bovine Mastitis

Cited by 10 publications

References 29 publications

Genomic and phenotypic characterization of Escherichia coli isolates recovered from the uterus of puerperal dairy cows

Genomic and phenotypic characterization of Escherichia coli isolates recovered from the uterus of puerperal dairy cows

Characterization of native Escherichia coli populations from bovine vagina of healthy heifers and cows with postpartum uterine disease

Treatment for bovineEscherichia colimastitis – an evidence‐based approach

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