Current advance of CRISPR/Cas-based SERS technology

Wang, Huimin; Su, Ai-Ling; Chang, Jingjing; Liang, Chaozhao; Xu, Shengyuan

doi:10.1039/d2sd00235c

Cited by 5 publications

(1 citation statement)

References 68 publications

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“…There are other approaches combining CRISPR/Cas-based MD and SERS that are not explicitly highlighted here. We refer the reader to a recent review summarizing publications on this emerging topic until the end of 2022 …”

Section: Next-generation Of Molecular Diagnostics: Convergence With Sersmentioning

confidence: 99%

Convergence of Surface-Enhanced Raman Scattering with Molecular Diagnostics: A Perspective on Future Directions

Choi,

Schlücker

2024

ACS Nano

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Molecular diagnostics (MD) is widely employed in multiple scientific disciplines, such as oncology, pathogen detection, forensic investigations, and the pharmaceutical industry. Techniques such as polymerase chain reaction (PCR) revolutionized the rapid and accurate identification of nucleic acids (DNA, RNA). More recently, CRISPR and its CRISPR-associated protein (Cas) have been a ground-breaking discovery that is the latest revolution in molecular biology, including MD. Surface-enhanced Raman scattering (SERS) is a very attractive alternative to fluorescence as the currently most widely used optical readout in MD. In this Perspective, milestones in the development of MD, SERS-PCR, and next-generation approaches to MD, such as Specific High-Sensitivity Enzymatic Reporter UnLOCKing (SHERLOCK) and DNA Endonuclease-Targeted CRISPR Trans Reporter (DETECTR), are briefly summarized. Our perspective on the future convergence of SERS with MD is focused on SERS-based CRISPR/Cas (SERS-CRISPR) since we anticipate many promising applications in this rapidly emerging field. We predict that major future developments will exploit the advantages of real-time monitoring with the superior brightness, photostability, and spectral multiplexing potential of SERS nanotags in an automated workflow for rapid assays under isothermal, amplification-free conditions.

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Section: Next-generation Of Molecular Diagnostics: Convergence With Sersmentioning

confidence: 99%

Convergence of Surface-Enhanced Raman Scattering with Molecular Diagnostics: A Perspective on Future Directions

Choi,

Schlücker

2024

ACS Nano

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LAMP-CRISPR/Cas12a-based impedimetric biosensor powered by Fe3O4@Au-(S-polyA-S)-Au for detection of SARS-CoV-2

Behnam Rad,

Hakimian,

Mohebbi

et al. 2024

Microchim Acta

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Rapid and accurate SERS assay of disease-related nucleic acids based on isothermal cascade signal amplifications of CRISPR/Cas13a system and catalytic hairpin assembly

Zhang,

Chen,

et al. 2024

Biosensors and Bioelectronics

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Current advance of CRISPR/Cas-based SERS technology

Abstract: In recent years, integrating clustered regularly interspaced short palindromic repeats (CRISPR)-associated nuclease (Cas) has emerged as a powerful genome editing system. In addition to genome editing, CRISPR/Cas technology has attracted...

Cited by 5 publications

References 68 publications

Convergence of Surface-Enhanced Raman Scattering with Molecular Diagnostics: A Perspective on Future Directions

Convergence of Surface-Enhanced Raman Scattering with Molecular Diagnostics: A Perspective on Future Directions

LAMP-CRISPR/Cas12a-based impedimetric biosensor powered by Fe3O4@Au-(S-polyA-S)-Au for detection of SARS-CoV-2

Rapid and accurate SERS assay of disease-related nucleic acids based on isothermal cascade signal amplifications of CRISPR/Cas13a system and catalytic hairpin assembly

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