Current advances, challenges and strategies for enhancing the biodegradation of plastic waste

He, Yuehui; Deng, Xilong; Jiang, Lei; Hao, Lijuan; Shi, Yong; Lyu, Mingsheng; Zhang, Lei; Wang, Shujun

doi:10.1016/j.scitotenv.2023.167850

Cited by 15 publications

(2 citation statements)

References 141 publications

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“…The capacity of phytoplankton species, such as diatoms and cyanobacteria, to transform NPLs has also been documented. Observations include the biodegradation of plastic materials through processes like fouling, corrosion, hydrolysis, and penetration, as along with the degradation of leaching components and the diffusion of pigment coloration into the polymers [76,77]. Furthermore, the activity of the algal PETaze enzyme in C. reinhardtii when acting on PET plastic demonstrated the potential for biological degradation with a high conversion rate [78].…”

Section: The Phytoplankton Feedback On Npls Bioavailability and Toxicitymentioning

confidence: 99%

Progress in Research on the Bioavailability and Toxicity of Nanoplastics to Freshwater Plankton

Slaveykova,

Marelja

2023

Microplastics

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The present review critically examines the advancements in the past 5 years regarding research on the bioavailability and toxicity of the nanoplastics (NPLs) to freshwater plankton. We discuss the recent progress in the understanding of adsorption, absorption, trophic transfer, and biological effects in phyto- and zooplankton induced by NPLs exposure. The influence of plankton on NPLs’ bioavailability via the excretion of biomolecules and formation of eco-corona is also examined. Despite important research developments, there are still considerable knowledge gaps with respect to NPLs’ bioavailability and trophic transfer by plankton as well as a potential adverse effect in natural aquatic systems. As plankton play a critical role in primary production, nutrient cycling, and food web structure, understanding the interactions between NPLs and plankton is essential in assessing the potential implications of NPLs pollution for aquatic ecosystem biodiversity and services.

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Section: The Phytoplankton Feedback On Npls Bioavailability and Toxicitymentioning

confidence: 99%

Progress in Research on the Bioavailability and Toxicity of Nanoplastics to Freshwater Plankton

Slaveykova,

Marelja

2023

Microplastics

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“…Bio-based plastic degradation through enzymatic processes and the subsequent utilization of its decomposition byproducts represent a promising recycling method, offering an alternative to chemical recycling that circumvents the necessity for high-temperature treatments. This approach involves converting polymers into monomers, holding considerable promise as a method requiring lower temperatures for plastic breakdown [3,4] . The most advanced research in enzyme-mediated plastic degradation is polyethylene terephthalate (PET).…”

Section: Introductionmentioning

confidence: 99%

A combination of two-enzyme system and enzyme engineering improved the activity of a new PET hydrolase identified from soil bacterial genome

Mabashi-Asazuma,

Hirai,

Sakurai

et al. 2024

Preprint

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We here report a novel PET hydrolase originating from a soil microbial genome sequence. This enzyme, bbPET0069, exhibits characteristics resembling a cutinase-like Type I PET-degrading enzyme but lacks disulfide bonds. Notably, bbPET0069 displayed remarkable synergy withCandida antarcticalipase B (CALB), demonstrating rapid and efficient PET degradation. To improve the PET degradation activity of bbPET0069, we employed a three-dimensional (3D) structural modeling to identify mutation sites around its substrate binding domain combined with a protein language model for effective mutation prediction. Through three initial rounds of directed evolution, we achieved a significant enhancement in PET degradation with CALB, resulting in a 12.6-fold increase compared to wild-type bbPET0069 without CALB. We confirmed its PET degradation activity in PET nanoparticles and films, and our proposed approach enabled efficient PET degradation to terephthalic acid monomers up to 95.5%. Our approach, which integrates a two-enzyme system with protein engineering, demonstrates the potential for enhancing the activity of emerging PET-degradation enzymes, which may possess unique attributes.Graphical AbstractA novel PET hydrolase, bbPET0069, was identified from a soil microbial genome. bbPET0069 and CALB showed remarkable synergy in PET degradation. Using surface feature analysis, PET degradation activity of bbPET0069 was significantly improved. This combination of a two-enzyme system and surface feature analysis holds promise for enhancing emerging PET-degradation enzymes.

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Cytochrome P450 for environmental remediation: catalytic mechanism, engineering strategies and future prospects

Yang,

Yu,

Qian

et al. 2023

World J Microbiol Biotechnol

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Current advances, challenges and strategies for enhancing the biodegradation of plastic waste

Cited by 15 publications

References 141 publications

Progress in Research on the Bioavailability and Toxicity of Nanoplastics to Freshwater Plankton

Progress in Research on the Bioavailability and Toxicity of Nanoplastics to Freshwater Plankton

A combination of two-enzyme system and enzyme engineering improved the activity of a new PET hydrolase identified from soil bacterial genome

Cytochrome P450 for environmental remediation: catalytic mechanism, engineering strategies and future prospects

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