Current Advances in 3D Bioprinting for Cancer Modeling and Personalized Medicine

Germain, Nicolas; Dhayer, Mélanie; Dekiouk, Salim; Marchetti, Philippe

doi:10.20944/preprints202202.0303.v1

Cited by 10 publications

(1 citation statement)

References 145 publications

(176 reference statements)

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“… 21 Furthermore, the use of ‘bio‐inks’ permits printing of various structures with filament‐based techniques at resolutions of 100 μm into collagen, Matrigel or decellularised matrix. 22 Plane‐based techniques are best suited to print higher resolution 3D structures, although these approaches are limited by available bio‐inks and large volume requirements making them relatively expensive. In general, bio‐inks allow for inclusion of cells during dispersal which can facilitate even distribution of cells throughout the 3D printed structure, generating an effective coculture system with fibroblasts, endothelial and/or immune cells.…”

Section: Discussionmentioning

confidence: 99%

A method for reproducible high‐resolution imaging of 3D cancer cell spheroids

Phillips

Caprettini

Aggarwal

et al. 2023

Journal of Microscopy

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Multicellular tumour cell spheroids embedded within three-dimensional (3D) hydrogels or extracellular matrices (ECM) are widely used as models to study cancer growth and invasion. Standard methods to embed spheroids in 3D matrices result in random placement in space which limits the use of inverted fluorescence microscopy techniques, and thus the resolution that can be achieved to image molecular detail within the intact spheroid. Here, we leverage UV photolithography to microfabricate PDMS (polydimethylsiloxane) stamps that allow for generation of high-content, reproducible well-like structures in multiple different imaging chambers. Addition of multicellular tumour spheroids into stamped collagen structures allows for precise positioning of spheroids in 3D space for reproducible high-/super-resolution imaging. Embedded spheroids can be imaged live or fixed and are amenable to immunostaining, allowing for greater flexibility of experimental approaches. We describe the use of these spheroid imaging chambers to analyse cell invasion, cell-ECM interaction, ECM alignment, force-dependent intracellular protein dynamics and extension of fine actin-based protrusions with a variety of commonly used inverted microscope platforms. This method enables reproducible, high-/super-resolution live imaging of multiple tumour spheroids, that can be potentially extended to visualise organoids and other more complex 3D in vitro systems.

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Section: Discussionmentioning

confidence: 99%

A method for reproducible high‐resolution imaging of 3D cancer cell spheroids

Phillips

Caprettini

Aggarwal

et al. 2023

Journal of Microscopy

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Strategies for Improving Vascularization in Kidney Organoids: A Review of Current Trends

Konoe

Morizane

2023

Biology

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Kidney organoids possess the potential to revolutionize the treatment of renal diseases. However, their growth and maturation are impeded by insufficient growth of blood vessels. Through a PubMed search, we have identified 34 studies that attempted to address this challenge. Researchers are exploring various approaches including animal transplantation, organ-on-chips, and extracellular matrices (ECMs). The most prevalent method to promote the maturation and vascularization of organoids involves transplanting them into animals for in vivo culture, creating an optimal environment for organoid growth and the development of a chimeric vessel network between the host and organoids. Organ-on-chip technology permits the in vitro culture of organoids, enabling researchers to manipulate the microenvironment and investigate the key factors that influence organoid development. Lastly, ECMs have been discovered to aid the formation of blood vessels during organoid differentiation. ECMs from animal tissue have been particularly successful, although the underlying mechanisms require further research. Future research building upon these recent studies may enable the generation of functional kidney tissues for replacement therapies.

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High Hopes for the Biofabrication of Articular Cartilage—What Lies beyond the Horizon of Tissue Engineering and 3D Bioprinting?

Sbirkov,

Redzheb,

Forraz

et al. 2024

Biomedicines

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Technologies and biomaterials for 3D bioprinting have been developing extremely quickly in the past decade as they hold great potential in tissue engineering. This, together with the possibility to differentiate stem cells of different origin into any cell type, raises the hopes in regenerative medicine once again after the initial breakthrough with stem cells in the 1980s. Nevertheless, three decades of 3D bioprinting experiments have shown that the production of functional tissues would take a longer time than anticipated. Cartilage, one of the simplest tissues in the body, consists of only one cell type. It is not vascularised and innervated and does not have lymphatic vessels either, which makes it a perfect target tissue for successful implantation. The tremendous amount of work since the beginning of this century, combining the efforts of bioengineers, material scientists, biologists, and physicians, has culminated in multiple proof-of-concept constructs that have been implanted in animals. However, there is no single reproducible, standardised, widely accessible and accepted strategy that can be readily applied in the clinic. In this review, we focus on the current progress in the field of the 3D biofabrication of articular cartilage and critically assess failures and future challenges.

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Current Advances in 3D Bioprinting for Cancer Modeling and Personalized Medicine

Cited by 10 publications

References 145 publications

A method for reproducible high‐resolution imaging of 3D cancer cell spheroids

A method for reproducible high‐resolution imaging of 3D cancer cell spheroids

Strategies for Improving Vascularization in Kidney Organoids: A Review of Current Trends

High Hopes for the Biofabrication of Articular Cartilage—What Lies beyond the Horizon of Tissue Engineering and 3D Bioprinting?

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