Current advances in antibody‐based serum biomarker studies: From protein microarray to phage display

Abstract

Purpose This review aims to summarize the technological advances in the field of antibody‐based biomarker studies by proteome microarray and phage display. In addition, the possible development directions of this field are also discussed. Experimental design We have focused on the antibody profiling by proteome microarray and phage display, including the technological advances, the tools/resources constructed, and the characteristics of both platforms. Results With the help of tools/resources and technological advances in proteome microarray and phage display, the efficiency of profiling antibody‐based biomarkers in serum samples has been greatly improved. Conclusions In the past few years, proteome microarray and phage display, especially the latter one, have already demonstrated their capacity and efficiency for biomarker identification. In the near future, we believe that more antibody‐based biomarkers could be identified, and some of them could eventually be developed into real clinical applications.

Advances in plasma proteomics: Moving from technology to precision medicine

“…Qi et al reviewed the technological advances in the field of autoantibody studies by proteome microarray and phage display, discussed their merits and limitations and the future directions of this field. [15] Following the advances of genomics, proteomics has had a growing influence on precision medicine by elucidating patient heterogeneity and finding biomarkers for more precise disease detection as well as the targets for developing more effective therapies. [16] Studying the circulating proteomics will represent the frontier of clinical proteomics.…”

“…reviewed the technological advances in the field of autoantibody studies by proteome microarray and phage display, discussed their merits and limitations and the future directions of this field. [ 15 ]…”

Phage Display in Cancer Research: Special Issue Editorial

“…Soon after its birth in 1985, following a short lag period [ 1 , 2 ], the phage display technology enjoyed a tremendous exponential growth, demonstrating its competence and performance in solving numerous challenging problems in material science, bioengineering, and medicine, including cancer research [ 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 ] ( Figure 1 ). This Special Issue aims to illustrate the promise of phage display as a universal research tool in cancer research.…”