Current and future editing reagent delivery systems for plant genome editing

Ran, Yidong; Liang, Zhen; Gao, Caixia

doi:10.1007/s11427-017-9022-1

Cited by 136 publications

(76 citation statements)

References 131 publications

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“…SPL2, SPL10, and SPL11 constitute another miR156-targeted SPL subfamily. Because SPL10 and SPL11 are located in the same genetic locus, we generated spl10 spl11 double mutants by the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 technology (Supplemental Figures 2A to 2C;Ran et al, 2017;Yin et al, 2017). Compared to wild type, spl10 spl11 showed increased rooting capacity (Figures 1A to 1C;Supplemental Figures 2D to 2I).…”

Section: Divergent Roles Of Mir156-targeted Spls In Plant Regenerationmentioning

confidence: 99%

AP2/ERF Transcription Factors Integrate Age and Wound Signals for Root Regeneration

et al. 2019

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Age and wounding are two major determinants for regeneration. In plants, the root regeneration is triggered by woundinduced auxin biosynthesis. As plants age, the root regenerative capacity gradually decreases. How wounding leads to the auxin burst and how age and wound signals collaboratively regulate root regenerative capacity are poorly understood. Here, we show that the increased levels of three closely-related miR156-targeted Arabidopsis (Arabidopsis thaliana) SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factors, SPL2, SPL10, and SPL11, suppress root regeneration with age by inhibiting wound-induced auxin biosynthesis. Mechanistically, we find that a subset of APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF) transcription factors including ABSCISIC ACID REPRESSOR1 and ERF109 is rapidly induced by wounding and serves as a proxy for wound signal to induce auxin biosynthesis. In older plants, SPL2/10/11 directly bind to the promoters of AP2/ERFs and attenuates their induction, thereby dampening auxin accumulation at the wound. Our results thus identify AP2/ERFs as a hub for integration of age and wound signal for root regeneration.

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Section: Divergent Roles Of Mir156-targeted Spls In Plant Regenerationmentioning

confidence: 99%

AP2/ERF Transcription Factors Integrate Age and Wound Signals for Root Regeneration

et al. 2019

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“…A prerequisite for successful CRISPR/Cas9-mediated plant gene editing is the availability of efficient delivery methods for the gene editing components and a satisfactory plant regeneration system (Altpeter et al, 2016 ; Ran et al, 2017 ). Although many successful reports of CRISPR-mediated plant gene editing have now been published, there are a number of factors which affect efficiency.…”

Section: Challenges Pertaining To Application Of Crispr/cas9 In Tropimentioning

confidence: 99%

Application of CRISPR/Cas9 Genome Editing Technology for the Improvement of Crops Cultivated in Tropical Climates: Recent Progress, Prospects, and Challenges

et al. 2018

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The world population is expected to increase from 7.3 to 9.7 billion by 2050. Pest outbreak and increased abiotic stresses due to climate change pose a high risk to tropical crop production. Although conventional breeding techniques have significantly increased crop production and yield, new approaches are required to further improve crop production in order to meet the global growing demand for food. The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 (CRISPR-associated protein9) genome editing technology has shown great promise for quickly addressing emerging challenges in agriculture. It can be used to precisely modify genome sequence of any organism including plants to achieve the desired trait. Compared to other genome editing tools such as zinc finger nucleases (ZFNs) and transcriptional activator-like effector nucleases (TALENs), CRISPR/Cas9 is faster, cheaper, precise and highly efficient in editing genomes even at the multiplex level. Application of CRISPR/Cas9 technology in editing the plant genome is emerging rapidly. The CRISPR/Cas9 is becoming a user-friendly tool for development of non-transgenic genome edited crop plants to counteract harmful effects from climate change and ensure future food security of increasing population in tropical countries. This review updates current knowledge and potentials of CRISPR/Cas9 for improvement of crops cultivated in tropical climates to gain resiliency against emerging pests and abiotic stresses.

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“…Therefore, to ensure effective point mutations in a target gene, HDR-mediated genome-editing technique has been widely applied in different organisms. Despite this, its application is somehow limited in plants owing to the limited availability of donor templates in plant cells [7,15,16]. However, recent development of potential CRISPR/Cas9 gene targeting technology found helpful for homozygous recombination in plants [17].…”

Section: Introductionmentioning

confidence: 99%

Base Editing: The Ever Expanding Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Tool Kit for Precise Genome Editing in Plants

Monsur

Shao

et al. 2020

Genes

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Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated protein 9 (Cas9), a newly developed genome-editing tool, has revolutionized animal and plant genetics by facilitating modification of target genes. This simple, convenient base-editing technology was developed to improve the precision of genome editing. Base editors generate precise point mutations by permanent base conversion at a specific point, with very low levels of insertions and deletions. Different plant base editors have been established by fusing various nucleobase deaminases with Cas9, Cas13, or Cas12a (Cpf1), proteins. Adenine base editors can efficiently convert adenine (A) to guanine (G), whereas cytosine base editors can convert cytosine (C) to thymine (T) in the target region. RNA base editors can induce a base substitution of A to inosine (I) or C to uracil (U). In this review, we describe the precision of base editing systems and their revolutionary applications in plant science; we also discuss the limitations and future perspectives of this approach.

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Current and future editing reagent delivery systems for plant genome editing

Cited by 136 publications

References 131 publications

AP2/ERF Transcription Factors Integrate Age and Wound Signals for Root Regeneration

AP2/ERF Transcription Factors Integrate Age and Wound Signals for Root Regeneration

Application of CRISPR/Cas9 Genome Editing Technology for the Improvement of Crops Cultivated in Tropical Climates: Recent Progress, Prospects, and Challenges

Base Editing: The Ever Expanding Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Tool Kit for Precise Genome Editing in Plants

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