Current bioanalytical methods for detection of penicillins

Babington, Ruth; Matas, Sonia; Marco, M.‐Pilar; Galvé, Roger

doi:10.1007/s00216-012-5960-4

Cited by 61 publications

(30 citation statements)

References 60 publications

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“…Research labs and companies have been developing tests for antibiotics in milk that can be used in the barn or at the dairy processing facility for over 30 years, and excellent reviews are available (Davis and Higson, 2010; Samsonova et al, 2012; Babington et al, 2012; Danaher and Jordan, 2013). Key issues for continued improvement include elimination of any sample preparation, minimized use of complex equipment, and multiplexed analysis to detect multiple antibiotics simultaneously.…”

Section: Veterinary Medicinementioning

confidence: 99%

Point-of-care diagnostics for niche applications

Cummins

Ligler

Walker

2016

Biotechnology Advances

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Point-of-care or point-of-use diagnostics are analytical devices that provide clinically relevant information without the need for a core clinical laboratory. In this review we define point-of-care diagnostics as portable versions of assays performed in a traditional clinical chemistry laboratory. This review discusses five areas relevant to human and animal health where increased attention could produce significant impact: veterinary medicine, space travel, sports medicine, emergency medicine, and operating room efficiency. For each of these areas, clinical need, available commercial products, and ongoing research into new devices are highlighted.

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Section: Veterinary Medicinementioning

confidence: 99%

Point-of-care diagnostics for niche applications

Cummins

Ligler

Walker

2016

Biotechnology Advances

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“…Generating specific antibodies against the ß-lactam ring seems to remain the bottleneck for the development of such an immunoassay, due to the in-vitro instability and chemical reactivity of antibodies. Typically, antibodies against penicillins, which are described so far, are either highly specific or have limited group specificity for a few closely related compounds 39, 40 . To tackle this issue, we took advantage of one target of ß-lactam antibiotics, the PBP3 from S. pneumoniae , which is generic as well as highly specific towards the active form of ß-lactams.…”

Section: Discussionmentioning

confidence: 99%

Clinical on-site monitoring of ß-lactam antibiotics for a personalized antibiotherapy

Bruch

Chatelle

Kling

et al. 2017

Sci Rep

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An appropriate antibiotherapy is crucial for the safety and recovery of patients. Depending on the clinical conditions of patients, the required dose to effectively eradicate an infection may vary. An inadequate dosing not only reduces the efficacy of the antibiotic, but also promotes the emergence of antimicrobial resistances. Therefore, a personalized therapy is of great interest for improved patients’ outcome and will reduce in long-term the prevalence of multidrug-resistances. In this context, on-site monitoring of the antibiotic blood concentration is fundamental to facilitate an individual adjustment of the antibiotherapy. Herein, we present a bioinspired approach for the bedside monitoring of free accessible ß-lactam antibiotics, including penicillins (piperacillin) and cephalosporins (cefuroxime and cefazolin) in untreated plasma samples. The introduced system combines a disposable microfluidic chip with a naturally occurring penicillin-binding protein, resulting in a high-performance platform, capable of gauging very low antibiotic concentrations (less than 6 ng ml−1) from only 1 µl of serum. The system’s applicability to a personalized antibiotherapy was successfully demonstrated by monitoring the pharmacokinetics of patients, treated with ß-lactam antibiotics, undergoing surgery.

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“…b-Lactam antibiotics have been the most widely used antimicrobial drugs for more than 80 years and still constitute the most important group of antibiotics [1]. Even the b-lactam which has poor stability is not possible to be excluded from the environment [2].…”

Section: Introductionmentioning

confidence: 99%