Current challenges and future perspectives on detection of geminiviruses

Shahmohammadi, Niayesh; Mansourpour, Mahsa; Golnaraghi, Alireza

doi:10.1016/b978-0-323-90587-9.00020-1

Cited by 4 publications

(2 citation statements)

References 181 publications

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“…During real seed transmission, plant viruses infect the embryo of fertilized seeds and later the seedlings of the next generation, whereas seed-borne viruses may invade any seed tissue. Both cases require viruses to reach the reproductive organs of infected plants, and many studies have reported high geminiviral loads in plant flowers and fruits [59], as well as the presence of these viruses in whole seeds [35]. Accordingly in this work, ToLCNDV was detected in different parts of male and female flowers.…”

Section: Discussionmentioning

confidence: 58%

Further Molecular Diagnosis Determines Lack of Evidence for Real Seed Transmission of Tomato Leaf Curl New Delhi Virus in Cucurbits

Sáez,

Kheireddine,

García

et al. 2023

Plants

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Begomoviruses (family Geminiviridae) cause serious diseases in many crop families. Since 2013, the Spanish isolate of tomato leaf curl New Delhi virus (ToLCNDV) has been a limiting factor for cucurbits production in the Mediterranean basin, forcing farmers to adapt new management and control techniques. Although it is well-known that begomoviruses are naturally transmitted by the whitefly Bemisia tabaci, the capacity of these viruses to be vertically transmitted through seeds remains controversial. Clarifying the potential ToLCNDV seed transmission is essential to understand the epidemiology of this threating-for-cucurbits virus and to design appropriate control strategies. We assessed ToLCNDV distribution in the leaves, flowers and seeds of the infected plants of susceptible Cucumis melo accessions and toleration to the infected genotypes of Cucurbita moschata by conventional and quantitative PCR. We analyzed whether the viral particle was transmitted to offspring. We also evaluated ToLCNDV presence in commercial seeds of cucurbits (zucchini (Cucurbita pepo), melon (C. melo), cucumber (Cucumis sativus) and watermelon (Citrullus lanatus)) and in their progenies. As the assayed seedlings remained symptomless, we increased the reliability and accuracy of detection in these samples by searching for replicative forms of ToLCNDV by combining Southern blot hybridization and rolling-circle amplification (RCA). However, integral genomic DNA was not identified in the plants of offspring. Although the seedborne nature of ToLCNDV was confirmed, our results do not support the transmission of this virus from contaminated seeds to progeny.

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Section: Discussionmentioning

confidence: 58%

Further Molecular Diagnosis Determines Lack of Evidence for Real Seed Transmission of Tomato Leaf Curl New Delhi Virus in Cucurbits

Sáez,

Kheireddine,

García

et al. 2023

Plants

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“…Both cases require that viruses arrive to reproductive organs of infected plants. Many studies have reported high geminiviral loads in plant flowers and fruits (Shahmohammadi et al, 2022), as well as the presence of these viruses in whole seeds . In accordance, in this work ToLCNDV was detected in different parts of male and female flowers and seeds.…”

Section: Discussionmentioning

confidence: 99%

First report of Cucurbit Chlorotic Yellows Virus (CCYV) and Tomato Leaf Curl New Delhi Virus (ToLCNDV) in Algeria and lack of evidence for seed transmission of ToLCNDV in melon and pumpkin.

Kheireddine

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Emerging plant viruses in the Mediterranean Basin have arisen in the last decades becoming an important threat to crop production in this region. The spread of new viral diseases has become one of the main factors limiting agronomic and economic yields of cultivated species of Cucurbitaceae family, the second in importance after Solanaceae. A wide cluster of elements modulates and favors the emergence of these pathogens, including biological, ecological, social, and cultural factors. Global warming favors vector movement to new geographies, while global commercial networks lead to germplasm trade among countries, allowing the long-distance dissemination of viruses. Moreover, the intensive agricultural model, in which prevail genetically homogeneous cultivars, prompts pathogens outbreaks within new geographic areas at a fast rate.One of the key components to develop control strategies, and secure horticultural production, is to identify the type and distribution of viruses present in crops. However, such information in Algeria is scarce and very limited. The objective of this Doctoral Thesis was to identify and determine the prevalence and distribution of the most common viruses on cultivated cucurbit plants (zucchini, cucumber, and melon), and get information about their genetic variability and mode of dispersion.In this work, cucurbit chlorotic yellows virus (CCYV) was first detected infecting cucumber and zucchini in Algeria. CCYV (genus Crinivirus, family Closteroviridae) is part of a complex of whitefly-transmitted viruses that cause yellowing disease in cucurbits. Determination of the complete CP, and partial RdRp and Hsp70 sequences of an Algerian CCYV isolate was conducted to unveil the evolutionary relationships with the published isolates in databases. The phylogenetic analysis showed that the Algerian isolate clustered into group I together with the majority of the reported CCYV isolates. The genetic variation within this group was very low (nucleotide identity higher than 99.5%) despite their extensive and discontinuous geographical distribution, plant species (cultivated or weeds) and year of collection. This low genetic divergence could be due to the recentness of this virus that did not face major changes for the present time. In our case, the presence of CCYV was only detected in mixed infection with tomato leaf curl New Delhi virus (ToLCNDV). In future works, it will be interesting to check the likely synergistic or antagonistic interaction between these two viruses that may affect the symptoms development and spread between crops. SUMMARY viiiToLCNDV (genus Begomovirus, family Geminiviridae) was also first detected infecting cucurbit plants in Algeria. ToLCNDV is a bipartite begomovirus that causes destructive epidemics on economically valuable cucurbit and solanaceous crops. The complete DNA-A and DNA-B genomic sequences of a ToLCNDV isolate from cucumber (ToLCNDV-Biskra) were obtained.Alignments of the nucleotide sequences of DNA-A and DNA-B revealed identities of 98.7 and 97.6%, respectively, ...

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First report of cotton leaf curl Gezira virus infecting Malva parviflora and in Iraq

Shahmohammadi

Dizadji

Al-Waeli

et al. 2023

Australasian Plant Dis. Notes

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Current challenges and future perspectives on detection of geminiviruses

Cited by 4 publications

References 181 publications

Further Molecular Diagnosis Determines Lack of Evidence for Real Seed Transmission of Tomato Leaf Curl New Delhi Virus in Cucurbits

Further Molecular Diagnosis Determines Lack of Evidence for Real Seed Transmission of Tomato Leaf Curl New Delhi Virus in Cucurbits

First report of Cucurbit Chlorotic Yellows Virus (CCYV) and Tomato Leaf Curl New Delhi Virus (ToLCNDV) in Algeria and lack of evidence for seed transmission of ToLCNDV in melon and pumpkin.

First report of cotton leaf curl Gezira virus infecting Malva parviflora and in Iraq

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