Current characteristics of<i>λ</i>-DNA molecules/polystyrene nanoparticles in TBE buffer solution through micro/nanofluidic capillaries under DC electric field

Duan, Yifei; Zhao, Wei; Xue, Jing; Wang, Kaige; Wang, Guiren; Li, Junjie; Bai, Jintao; Gu, Chen

doi:10.1088/1361-6463/aa5c61

Cited by 3 publications

(8 citation statements)

References 29 publications

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“…The magnitude of electric conductance in subregion I is apparently higher than that in subregion II. This is consistent with our previous investigations . Similar two-subregion behaviors of the I s – V relation were also observed under pH = 6, 8, and 9, as shown in Figure .…”

Section: Experimental Resultssupporting

confidence: 93%

“…The cathode area ( S C ) was 20 times greater than the anode area ( S W ). The area ratio ( S C / S W ) between the cathode and anode was consistent with that used by Duan et al, who showed an interesting and abnormal “fall-off” in electric conductance. This is why an area ratio of 20 was used in the subsequent investigation.…”

Section: Experimental Setup and Proceduressupporting

confidence: 89%

“…This is consistent with our previous investigations. 8 Similar two-subregion behaviors of the I s −V relation were also observed under pH = 6, 8, and 9, as shown in Figure 5.…”

Section: ■ Experimental Resultssupporting

confidence: 72%

“…6,7 The effects of bioelectricity on fluids and surface characteristics at the nanoscale, although important, remain poorly understood. For example, in our previous investigation, 8 we observed a sudden fall-off in electric conductance in a nanocapillary with a diameter of 200 nm. This phenomenon was attributed to the shear-thickening effect of a Tris-borate−ethylenediaminetetraacetic acid (EDTA) (TBE) solution under a direct current (DC) electric field, i.e., a kind of rheological effect.…”

Section: ■ Introductionmentioning

confidence: 63%

“…In biomedical and life science research, electrokinetic flows produced by or occurring within living organisms not only rely on the cell membrane net charge (polarization) but also induce variations in local biophysical and chemical properties at the nanometer scale along the membrane surfaces of single cells, which in turn triggers the downstream signaling pathways. , The effects of bioelectricity on fluids and surface characteristics at the nanoscale, although important, remain poorly understood. For example, in our previous investigation, we observed a sudden fall-off in electric conductance in a nanocapillary with a diameter of 200 nm. This phenomenon was attributed to the shear-thickening effect of a Tris-borate–ethylenediaminetetraacetic acid (EDTA) (TBE) solution under a direct current (DC) electric field, i.e., a kind of rheological effect.…”

Section: Introductionmentioning

confidence: 75%

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Abnormal Rheological Phenomena in Newtonian Fluids in Electroosmotic Flows in a Nanocapillary

et al. 2018

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Abnormal rheological phenomena arising in Tris-borate−ethylenediaminetetraacetic acid solutions (believed to be Newtonian fluids) were observed in direct current electroosmotic flows within a nanocapillary with a diameter of 200 nm under a low electric field of tens of volts per meter. In solutions with different concentrations and pH values, the flow behavior indices of the power-law fluids were calculated on the basis of current−voltage relations. When the electric field intensity was below a critical value of 6.7 V/m, the fluids exhibited dilatant (shear thickening) effects. Fluid viscosity changed with electric field intensity because the nearwall shear rate of an electroosmotic flow changes with electric field intensity via a power-law relation. When the electric field intensity surpasses the critical electric field, the fluid again becomes Newtonian and has constant viscosity. The investigation shows that in nanocapillaries, fluids commonly believed to be Newtonian can become non-Newtonian near walls as a result of strong nanoscale interfacial effects. The results can also improve our understanding of electroosmosis-related transport phenomena in nanofluidics and biomedical science.

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Section: Experimental Resultssupporting

confidence: 93%

Section: Experimental Setup and Proceduressupporting

confidence: 89%

“…This is consistent with our previous investigations. 8 Similar two-subregion behaviors of the I s −V relation were also observed under pH = 6, 8, and 9, as shown in Figure 5.…”

Section: ■ Experimental Resultssupporting

confidence: 72%

Section: ■ Introductionmentioning

confidence: 63%

Section: Introductionmentioning

confidence: 75%

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Abnormal Rheological Phenomena in Newtonian Fluids in Electroosmotic Flows in a Nanocapillary

et al. 2018

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Effect of Mono- and Divalent Metal Ions on Current–Voltage Features of a λ-DNA Solution Electrically Driven in a Microfluidic Capillary

et al. 2022

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The interactions of DNA molecules and metal ions lead to changes in their configuration and conformation, which in turn influence the current characteristics of the solution as DNA molecules are translocated through a micro/nanofluidic channel and ultimately cause serious impacts on the practical applications of DNA/gene chips for precisely manipulating and studying the molecular properties of single DNA molecules. In this study, the current characteristics of λ-DNA solutions without or with metal ions (i.e., K + , Na + , Mg 2+ , and Ca 2+ ) were experimentally investigated when they were transported through a 5 μm microcapillary under an external electric field with asymmetric electrodes. Experimental data indicated some meaningful results. First, the current−voltage relations of the metal ion solutions were all linear, while those of λ-DNA solutions without or with metal ions were all nonlinear and followed power functions, of which the indices were related to the type, valence, and mobility of ions. Furthermore, as the concentrations of metal ions increased, the power indices of the λ-DNA solutions with monovalent metal ions increased, while those of the λ-DNA solutions with divalent ions decreased. Finally, the main reasons for the current characteristics were theoretically attributed to two possible mechanisms: the polarizations on the asymmetric electrodes and the interactions between λ-DNA and metal ions. These findings are helpful for the design of new biomedical micro/nanofluidic sensors and labs on a chip for accurately manipulating single DNA molecules.

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Effect of mono-/divalent metal ions on the conductivity characteristics of DNA solutions transferring through a microfluidic channel

Zhu 朱,

Xue 薛,

Zhao 赵

et al. 2023

Chinese Phys. B

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Interactions between deoxyribonucleic acid (DNA) and metal ions are vital for maintaining life functions, however, there are still unsolved questions about its mechanisms. It is of great practical significance to study these issues for medical chip design, drug development, health care, etc. In this investigation, the conductivity properties of λ-DNA solutions with mono-/divalent metal ions (Na+, K+, Mg2+, and Ca2+) were experimentally studied as they were electrically driven through a 5 μm microfluidic channel. Experimental data indicated that the conductivities of λ-DNA solutions with metal ions (M+/M2+) basically tend to reduce firstly and then increase as the voltage increases, of which the turning points varied with the metal ions. When the voltage surpasses turning points, the conductivity of λ-DNA-M+ solutions increased with the concentration of metal ions, while that of λ-DNA-M2+ solutions decreased. Moreover, the conductivity of λ-DNA-M2+ solutions was always smaller than that of λ-DNA-M+ solutions, and with high-concentration M2+, it is even smaller than that of the λ-DNA solution. The main reasons for the above findings could be attributed to the polarization of electrodes and different mechanisms of interactions between metal ions and λ-DNA molecules. This investigation is helpful for the precise manipulation of single DNA molecules in micro-/nanofluidic space and the design of new biomedical micro-/nanofluidic sensors.

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Current characteristics ofλ-DNA molecules/polystyrene nanoparticles in TBE buffer solution through micro/nanofluidic capillaries under DC electric field

Cited by 3 publications

References 29 publications

Abnormal Rheological Phenomena in Newtonian Fluids in Electroosmotic Flows in a Nanocapillary

Abnormal Rheological Phenomena in Newtonian Fluids in Electroosmotic Flows in a Nanocapillary

Effect of Mono- and Divalent Metal Ions on Current–Voltage Features of a λ-DNA Solution Electrically Driven in a Microfluidic Capillary

Effect of mono-/divalent metal ions on the conductivity characteristics of DNA solutions transferring through a microfluidic channel

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