Current Clinical and Pharmaceutical Applications of Microarrays: From               Disease Biomarkers Discovery to Automated Diagnostics

Lagraulet, Adriana

doi:10.1016/j.jala.2010.06.011

Cited by 9 publications

(6 citation statements)

References 65 publications

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“…Surface-bound biomolecules have gained growing attention in the field of biotechnology and biomedicine. Important examples include heterogeneous immunoassays and biosensors , for the specific detection of dissolved antigens, DNA and peptide chips for genome analysis , and the analysis of enzymatic pathways, the replication of cell membranes by immobilized lipid films, or the control of cell adsorption onto surfaces by adhesion proteins . Moreover, nonspecifically adsorbed proteins significantly affect the biocompatibility of implants .…”

Section: Introductionmentioning

confidence: 99%

Conformation and Activity of Glucose Oxidase on Homogeneously Coated and Nanostructured Surfaces

Seehuber

Dahint

2013

J. Phys. Chem. B

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Protein unfolding and loss of protein function upon surface contact is a major problem in biotechnology and biomedicine. Using glucose oxidase (GOx) as a model protein, we investigated the impact of surface chemistry, topography, and confinement on enzyme activity, conformation, and affinity. A particular focus lay on the question whether the conformation of surface-bound proteins can be stabilized by embedding nanoscale adsorption sites, here in the form of monodisperse gold nanoparticles (AuNPs), into a protein-repelling matrix material. It was found that on homogeneous surfaces, GOx activity is generally lower than that in its native state and strongly affected by surface chemistry. Loss of activity is related to an increasing amount of β-sheets in the GOx secondary structure and a corresponding reduction of α-helical elements. In contrast, on AuNP surfaces, the effect of surface chemistry is negligible, and the amount of adsorbed protein only depends on particle size. The low activity of GOx on all nanostructures studied is again accompanied by an increase of β-sheet and a reduction of α-helical secondary structure. The major cause for protein unfolding on AuNPs thus seems to be the curvature of the surface. In addition, the data suggest that unfavorable orientation of the adsorbed enzyme also contributes to the loss of activity.

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Section: Introductionmentioning

confidence: 99%

Conformation and Activity of Glucose Oxidase on Homogeneously Coated and Nanostructured Surfaces

Seehuber

Dahint

2013

J. Phys. Chem. B

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“…Accordingly, the detected fluorescence signal intensity reflects the presence of particular miRNAs and their relative expression levels in the tested sample. This method may also be used to compare the miRNAs profile between two samples using two different fluorescent dyes with different emission wavelengths (two-color microarray) [ [155] , [156] , [157] ]. Microarray-based method is particularly attractive for several factors.…”

Section: Mirnas As Potential Biomarkers In Pcamentioning

confidence: 99%

MicroRNAs as clinical tools for diagnosis, prognosis, and therapy in prostate cancer

Ghamlouche

Yehya

Zeid

et al. 2023

Translational Oncology

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“…For instance, thrombus formation on implants (and inflammatory responses) and hemodialysis membranes, biofouling of implanted devices and analytical chips intended for chronic use, and dental plaque formation are among the most common issues where protein adsorption leads to device failure. On the other hand, controlled protein adsorption is highly desirable for other devices and applications, such as biosensors and immunoassays, , genome analysis, protein separation and purification, membrane filtration, and scaffold vascularization . Effective control over the protein adsorption process necessitates understanding the underlying mechanisms and the involved interactions with the surface.…”

Section: Introductionmentioning

confidence: 99%

Interactions of Catalytic Enzymes with n-Type Polymers for High-Performance Metabolite Sensors

Ohayon

Renn

Wustoni

et al. 2023

ACS Appl. Mater. Interfaces

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The tight regulation of the glucose concentration in the body is crucial for balanced physiological function. We developed an electrochemical transistor comprising an n-type conjugated polymer film in contact with a catalytic enzyme for sensitive and selective glucose detection in bodily fluids. Despite the promise of these sensors, the property of the polymer that led to such high performance has remained unknown, with charge transport being the only characteristic under focus. Here, we studied the impact of the polymer chemical structure on film surface properties and enzyme adsorption behavior using a combination of physiochemical characterization methods and correlated our findings with the resulting sensor performance. We developed five n-type polymers bearing the same backbone with side chains differing in polarity and charge. We found that the nature of the side chains modulated the film surface properties, dictating the extent of interactions between the enzyme and the polymer film. Quartz crystal microbalance with dissipation monitoring studies showed that hydrophobic surfaces retained more enzymes in a densely packed arrangement, while hydrophilic surfaces captured fewer enzymes in a flattened conformation. X-ray photoelectron spectroscopy analysis of the surfaces revealed strong interactions of the enzyme with the glycolated side chains of the polymers, which improved for linear side chains compared to those for branched ones. We probed the alterations in the enzyme structure upon adsorption using circular dichroism, which suggested protein denaturation on hydrophobic surfaces. Our study concludes that a negatively charged, smooth, and hydrophilic film surface provides the best environment for enzyme adsorption with desired mass and conformation, maximizing the sensor performance. This knowledge will guide synthetic work aiming to establish close interactions between proteins and electronic materials, which is crucial for developing high-performance enzymatic metabolite biosensors and biocatalytic charge-conversion devices.

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Current Clinical and Pharmaceutical Applications of Microarrays: From Disease Biomarkers Discovery to Automated Diagnostics

Cited by 9 publications

References 65 publications

Conformation and Activity of Glucose Oxidase on Homogeneously Coated and Nanostructured Surfaces

Conformation and Activity of Glucose Oxidase on Homogeneously Coated and Nanostructured Surfaces

MicroRNAs as clinical tools for diagnosis, prognosis, and therapy in prostate cancer

Interactions of Catalytic Enzymes with n-Type Polymers for High-Performance Metabolite Sensors

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