Background: To promote organ, tissue donation as well as transplantation well sensitized health care providers have an important role. In the view of extended period of infancy of the same in India, a study has been conducted to explore knowledge, attitude, and practices among postgraduate medical students in Mumbai.Methods: All were explained nature of the study and informed written consent was obtained from those who are willing to participate. Data was collected through self-administered questionnaire from December 2014 to February 2015 and was entered, analysed in Microsoft Excel 2012. Institutional Ethical Committee approval for this study was obtained and confidentiality was maintained in this process.Results: Out of 228 postgraduate students who were given self - administered questionnaire, 163(71.49%) participants returned back the same. There was a fair knowledge about organ and tissues which can be donated as well as transplanted in the participants. However 49.69% of the respondents knew importance of time factor for safe organ preservation and 18.4% doctors could define near relative for organ donation and transplantation. Though 61.41% of the participants could correctly define brain death, only 36.19% could correctly name all members of authorized certifying committee for brain death. Majority of the participants (99.38%) supported the concept of organ donation however only 38.03% have ever counselled patients for organ donation, 11.04% were registered organ donors and 4% carried the same. Conclusions:Postgraduate medical students lacked detailed technical and legal aspects about organ, tissue donation and transplantation which need to be strengthened.