Current Concepts of Follicle-Stimulating Hormone Receptor Gene Regulation1

George, Jitu W.; Dille, Elizabeth; Heckert, Leslie L.

doi:10.1095/biolreprod.110.085043

Cited by 110 publications

(76 citation statements)

References 187 publications

(230 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…FSH acts through FSHR, induces Sertoli cell proliferation (Riera et al 2012), establishes the final Sertoli cell number (Singh & Handelsman 1996) and stimulates transcription and metabolic activity in Sertoli cells, which contributes to germ cell survival and development (George et al 2011). Our present results confirm the functionality of FSHR in our cell lines through the evaluation of proliferation activity with FSH supplementation.…”

Section: Sertoli Cell Lines From Adult Mouse Testissupporting

confidence: 82%

Establishment of adult mouse Sertoli cell lines by using the starvation method

Sato¹,

Yoshida²,

Nozawa³

et al. 2013

Reproduction

View full text Add to dashboard Cite

Sertoli cells were isolated from the testes of 6-week-old mice and stable Sertoli cell lines with higher proliferation rates were subcloned after starvation of primary cultured cells. After two rounds of this subcloning, 33 subcloned lines were selected on the basis of their proliferation rates. In addition, these subclones were screened according to their phagocytic activity and the characteristics of mature Sertoli cells, such as the expression of androgen receptors (ARs) and progesterone receptors, by using western blotting and immunocytochemical analysis, in addition to their morphology and proliferation rates. After the third round of subcloning, 12 subclones were selected for the final selection using RT-PCR for identification of genes specifically expressed by various testicular cells. Three clones were selected that expressed Sertoli-cell-specific genes, i.e. stem cell factor, clusterin, AR, a-inhibin, transferrin, Wilms' tumour-1, Mü llerian inhibitory substance, sex-determining region Y-box 9, FSH receptor (Fshr) and occludin; however, these clones did not express globulin transcription factor 1, steroidogenic factor or androgen-binding protein. These clones also expressed growth and differentiation factors that act on germ cells, such as leukaemia inhibitory factor, transforming growth factor b1 and basic fibroblast growth factor 2, but did not express c-kit (specific for germ cells), LH receptor and 3b-hydroxyl-dehydrogenase (specific for Leydig cells). Immunocytochemical data confirmed the expression of clusterin in these clones. Furthermore, the Bromodeoxyuridine incorporation assay confirmed the proliferation activity of these clones through Fshr after treatment with FSH. These clones are considered to be valuable tools for the study of Sertoli cell-specific gene expression and function. Reproduction (2013) 145 505-516

show abstract

Section: Sertoli Cell Lines From Adult Mouse Testissupporting

confidence: 82%

Establishment of adult mouse Sertoli cell lines by using the starvation method

Sato¹,

Yoshida²,

Nozawa³

et al. 2013

Reproduction

View full text Add to dashboard Cite

show abstract

“…Due to their predominant role with respect to Fshr transcription, the E-box and its binding proteins, USF1 and USF2, have received the greatest attention thus far [26]. USF1 and USF2 are members of the helix-loop-helix family that form both Thereafter, DAPA was performed by hybridizing nuclear extracts prepared from transfected KK-1 cells with biotinylated wild-type E-box/AP-1 probe (Ebox), or mutant oligomer devoid of E-box binding activity (E-box mt), or using excess cold consensus USF1 competitor (USF1_comp) together with biotinylated E-box/AP-1 probe.…”

Section: Discussionmentioning

confidence: 99%

The Aryl Hydrocarbon Receptor Regulates Mouse Fshr Promoter Activity Through an E-Box Binding Site1

Teino

Kuuse

Ingerpuu

et al. 2012

Biology of Reproduction

View full text Add to dashboard Cite

The aryl hydrocarbon receptor (AHR) mediates the toxicity of a variety of environmental chemicals. Apart from this, an understanding is emerging that the AHR has a fundamental role in female reproduction. Evidence suggests that AHR participates in regulation of follicle-stimulating hormone receptor (Fshr) transcript level in mouse ovary by binding to the promoter of this gene in vivo. The purpose of this study was to demonstrate the molecular interplay of the Fshr promoter involved in the transactivation by AHR in mouse granulosa cells. We found that AHR activates the Fshr promoter through the region from À209 to À99 bp. In this region, the importance of the E-box motif was revealed by site-directed mutagenesis followed by promoter analysis. By focusing on the DNA/protein interactions, we defined the fact that the intact E-box but not upstream transcription factor 1 (USF1), which is known to bind this motif, is necessary for AHR binding to mouse Fshr promoter. Furthermore, by constructing AHR mutants defective in DNA interaction, we confirmed the importance of DNA binding for AHR's ability to bind to and activate Fshr promoter. Collectively, the present study demonstrates that AHR modulates Fshr transactivation by its direct association through an E-box and not by recruitment via interaction with USFs. These observations suggest that although AHR and USF may respond to different signals, they compete on binding to the same E-box. Our data, together with that from one prior study suggesting involvement of E-box motif in AHR-mediated transcription, provide novel understanding of the way in which AHR may regulate its target genes through E-box sites.

show abstract

“…The FSH acts via FSH receptor (FSHR), which is a trans-membrane glycoprotein, and it is expressed on granulosa cells in the ovary [6]. This receptor is synthesized by a single-copy gene, located in the region 2p21-16 [7].…”

Section: Introductionmentioning

confidence: 99%

Ala307Thr and Asn680Ser Polymorphisms ofFSHRGene in Human Reproduction Outcomes

Trevisan¹,

Peluso²,

Cordts³

et al. 2014

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: It is known that some markers of ovarian stimulation can help to personalize the treatment, adjusting the dose of exogenous rFSH, thus preventing excessive wear of the patient. We aimed to evaluate Ala307Thr and Asn680Ser genotypes of the FSHR gene in infertile women and correlate the findings with the results of ovarian response and assisted reproduction outcomes. Methods: Cross-sectional study covering 149 infertile women submitted to assisted reproduction treatment. Genotyping of FSHR variants were performed using TaqMan methodology by real time PCR. FSH and estradiol were measured by ELFA. The data was analyzed statistically. Results: The frequencies of the FSHR Ala307Thr and Asn680Ser genotypes considering the ovarian hyper stimulation response also did not differ statistically. Considering assisted reproduction outcomes, we observed that the polymorphism Ala307Thr have a statistical difference for the number of MII oocytes and embryos (p=0,051 and p=0.037, respectively), which the genotype Ala/Ala showed more embryos. The polymorphisms did not determine the FSH and estradiol serum levels and the ovarian response in the assisted reproduction treatment. Conclusions: The polymorphisms Ala307Thr and Asn680Ser did not determine the FSH and estradiol serum levels and the ovarian response in the assisted reproduction treatment. However, we observed that the Ala307Thr may influence the number of embryos produced.

show abstract

Current Concepts of Follicle-Stimulating Hormone Receptor Gene Regulation1

Cited by 110 publications

References 187 publications

Establishment of adult mouse Sertoli cell lines by using the starvation method

Establishment of adult mouse Sertoli cell lines by using the starvation method

The Aryl Hydrocarbon Receptor Regulates Mouse Fshr Promoter Activity Through an E-Box Binding Site1

Ala307Thr and Asn680Ser Polymorphisms ofFSHRGene in Human Reproduction Outcomes

Contact Info

Product

Resources

About