Current controversies in prenatal diagnosis 1: Is aneuploidy testing by PGD indicated for all infertile patients undergoing IVF?

Harper, Joyce; Repping, Sjoerd; Hill, David L.

doi:10.1002/pd.2178

Cited by 2 publications

(3 citation statements)

References 30 publications

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“…It refers to a laboratory procedure to diagnostically analyze polar body, single blastomere, and blastocyst from biopsied embryos before the embryos are transferred, allowing the selection of normal embryos for transfer (Ouhibi et al, 2001;Harper et al, 2009). PGD can be used for detecting at-risk couples who have already had a child with a genetic defect or whose family has a history of specific inherited disorders such as single gene defects and chromosomal abnormalities (Thornhill et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Whole genome amplification in preimplantation genetic diagnosis

Zheng

Wang

et al. 2011

J. Zhejiang Univ. Sci. B

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Preimplantation genetic diagnosis (PGD) refers to a procedure for genetically analyzing embryos prior to implantation, improving the chance of conception for patients at high risk of transmitting specific inherited disorders. This method has been widely used for a large number of genetic disorders since the first successful application in the early 1990s. Polymerase chain reaction (PCR) and fluorescent in situ hybridization (FISH) are the two main methods in PGD, but there are some inevitable shortcomings limiting the scope of genetic diagnosis. Fortunately, different whole genome amplification (WGA) techniques have been developed to overcome these problems. Sufficient DNA can be amplified and multiple tasks which need abundant DNA can be performed. Moreover, WGA products can be analyzed as a template for multi-loci and multi-gene during the subsequent DNA analysis. In this review, we will focus on the currently available WGA techniques and their applications, as well as the new technical trends from WGA products.

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Section: Introductionmentioning

confidence: 99%

Whole genome amplification in preimplantation genetic diagnosis

Zheng

Wang

et al. 2011

J. Zhejiang Univ. Sci. B

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“…By using PGD the couple can start a pregnancy assured that the disease has not been transmitted to the child, and the risk of having to terminate the pregnancy to be avoid. However, due to technical complexity and high cost (~SR 21,000), PGD is at present available in a rather limited number of centres worldwide [6]. …”

Section: Introductionmentioning

confidence: 99%

“…Ovarian reserve has to be optimal because it is particularly important to obtain an adequate number of embryos. Indeed, there will be a selection among them and PGD implantation rate is not as high as the rate observed in classical IVF with ICSI [6]. …”

Section: Introductionmentioning

confidence: 99%

Preimplantation genetic diagnosis in Saudi Arabia

Abotalib

2013

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Preimplantation genetic diagnosis (PGD) testing is the practice of obtaining a cellular biopsy sample from a developing human oocyte or embryo, acquired via a cycle of in vitro fertilization (IVF); evaluating the genetic composition of this sample; and using this information to determine which embryos will be optimal for subsequent uterine transfer. PGD has become an increasingly useful adjunct to IVF procedures. The ability to provide couples who are known carriers of genetic abnormalities the opportunity to deliver healthy babies has opened a new frontier in reproductive medicine. The purpose of the PGD is enables us to choose which embryos will be implanted into the mother. In the present study 137 families who had undergone IVF at Habib Medical Centre, were enrolled for the PGD analysis. The couple visited the clinic for the sex selection, recurrent fetal loss and with the recurrent IVF failure. 802 embryos were tested by the biopsy method and 512 are found to be normal and 290 were abnormal embryos. In this study only 24% of the embryos were transferred and the remaining was not transferred because of the abnormalities or undesired sex of the embryos. The structural and numerical abnormalities were found to be 16.8%.

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Current controversies in prenatal diagnosis 1: Is aneuploidy testing by PGD indicated for all infertile patients undergoing IVF?

Cited by 2 publications

References 30 publications

Whole genome amplification in preimplantation genetic diagnosis

Whole genome amplification in preimplantation genetic diagnosis

Preimplantation genetic diagnosis in Saudi Arabia

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