Current Developments in Excipient Science

Kar, Manoranjan; Chourasiya, Yashu; Maheshwari, Rahul; Tekade, Rakesh K.

doi:10.1016/b978-0-12-817909-3.00002-9

Cited by 56 publications

(35 citation statements)

References 64 publications

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“…We speculate that not the density, but the higher viscosity, which determines a slower and weaker flow through the brain vessels, is responsible for the less frequent occurrence of dilated vessels (Ferreira et al, 2017; Takamura et al, 2012; Wilkes, 2017). Alternatively, since dilated vessels may arise as a freezing artifact, the less frequent occurrence in AS specimens could be related to a protective effect against this freezing artifact determined by alcohol, namely glycerol (Bhattacharya, 2018; Kar, Chourasiya, Maheshwari, & Tekade, 2019; Zhang et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Antigenicity is preserved with fixative solutions used in human gross anatomy: A mice brain immunohistochemistry study

Frigon

Dadar

Boire

et al. 2022

Preprint

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Background: Histology remains the gold standard to assess human brain biology, so ex vivo studies using tissue from brain banks are standard practice in neuroscientific research. However, a larger number of specimens could be obtained from gross anatomy laboratories. These specimens are fixed with solutions appropriate for dissections, but whether they also preserve brain tissue antigenicity is unclear. Therefore, we perfused mice brains with solutions used for human body preservation to assess and compare the tissue quality and antigenicity of the main cell populations. Methods: 28 C57BL/6J mice were perfused with: 4% formaldehyde (FAS, N=9), salt-saturated solution (SSS, N=9), and alcohol-solution (AS, N=10). The brains were cut into 40μm sections for antigenicity analysis and were assessed by immunohistochemistry of four antigens: neuronal nuclei (NeuN), glial fibrillary acidic protein (GFAP-astrocytes), ionized calcium binding adaptor molecule1 (Iba1-microglia), and myelin proteolipid protein (PLP). We compared the fixatives according to multiple variables: perfusion quality, ease of manipulation, tissue quality, immunohistochemistry quality, and antigenicity preservation. Results: The perfusion quality was better using FAS and worse using AS. The manipulation was very poor in SSS brains. FAS and AS fixed brains showed higher tissue and immunohistochemistry quality than the SSS brains. All antigens were readily observed in every specimen, regardless of the fixative solution. Conclusion: Solutions designed to preserve specimens for human gross anatomy dissections also preserve tissue antigenicity in different brain cells. This offers opportunities for the use of human brains fixed in gross anatomy laboratories to assess normal or pathological conditions.

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Section: Discussionmentioning

confidence: 99%

Antigenicity is preserved with fixative solutions used in human gross anatomy: A mice brain immunohistochemistry study

Frigon

Dadar

Boire

et al. 2022

Preprint

View full text Add to dashboard Cite

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“…The average value of the panelists' preference for the viscosity of the solution made from gelatin and chili powder was 5.18 (Figure 5), with 77% of the panelists liking the viscosity of the solution. Gelatin can form a gel at a concentration of 0.5% [24]. The addition of gelatin was 2.5% from the dilution of 20 g of gelatin into 100 ml which was then dissolved in 200 g of water.…”

Section: Fig 4 Respondents' Interest In Consuming Edible Packaging Ma...mentioning

confidence: 99%

Consumer perceptions of edible packaging made of gelatin as chili powder packaging

et al. 2022

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Edible packaging is a massive trend product which production will influence new perceptions about packaging. There has not been much research on consumers’ perceptions of edible films, so it is necessary to determine the acceptance. Chili powder packaging is one of the edible film applications in the food industry. Edible packaging applications replacing chili powder plastic packaging can reduce plastic consumption, increase the feasibility and maintain chili powder quality. However, certain materials utilization can affect the perception, such as gelatin. Unsavory perception about gelatin edible packaging, such as its halal legality, will detain its production and development. Thus, this research aimed to analyze consumers’ perception of edible packaging made of gelatin as chili powder packaging. This research was conducted using a survey method by Google Forms questionnaire working 215 respondents and organoleptic preference test employing randomly selected 101 untrained panelists. Based on observations, most respondents have positive knowledge and perception about edible packaging made of gelatin as chili powder packaging. The panelists accepted the product color, viscosity, aroma, and taste, respectively 87%, 77%, 72%, and 69%. Taken together, the findings highlight the perception and acceptance of gelatin edible packaging to replace chilli powder undegradable packaging.

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“…Although, in comparison with commercial depilatories, DEPIKER 51 started depilation slower but had more depilatory effect. The cream base consists of liquid paraffin which serves as emollient and meets the standard of nontoxicity and non-irritancy (Mousumi et al, 2019) to prevent any possible skin irritation and itching that may be experienced by the test objects.…”

Section: Dehairing Function Of Formulated Depilatory Cream (Depiker 51) On Wistar Ratmentioning

confidence: 99%

Hair Removal Potential of Keratinase Produced by Bacillus Species Isolated From Feather Waste

DADA

Wakil²

2021

J microb biotech food sci

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Keratins are tough, fibrous proteins found as the major protein component in hair. Chemical hair removal is harsh and results in severe toxicity and pollution, hence, a need for a non-chemical enzymatic hair removal process. The aim of this study is to characterise keratinase from isolated keratin-degrading Bacillus licheniformis-K51, Bacillus subtilis-K50 and Bacillus sp.-K53 and investigate its potential in hair removal. The collagenolytic ability of keratin-degrading keratinases obtained from Bacillus licheniformis-K51, Bacillus subtilis-K50 and Bacillus sp.-K53 was determined using standard methods. The depilatory potential of keratinase (directly and as part of the constituent of formulated depilatory cream) was determined in vitro on goat skin and in vivo on Wistar rat. Purified keratinases from Bacillus licheniformis-K51 (EZYKer-51), Bacillus subtilis-K50 (EZYKer-50) and Bacillus sp.-K53 (EZYKer-53) did not show significant collagenolytic activities. In vitro treatment of goat skin with EZYKer-51 showed total hair removal within 24 hours and partial hair removal with EZYKer-50 and EZYKer-53. The formulated depilatory cream containing EZYKer-51 showed highest keratinolytic activity (22.20±0.63 U/mL) followed by EZYKer-50 (20.52±0.46 U/mL) and EZYKer-53 (17.06±0.81 U/mL). In vivo depilatory action using formulated cream took 40 min, while that of commercial cream was achieved within 20 min. Keratinase from Bacillus licheniformis-K51 exhibited complete hair-removal function which makes it potentially suitable in industrial processes involving removal of hair. ARTICLE INFO

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Current Developments in Excipient Science

Cited by 56 publications

References 64 publications

Antigenicity is preserved with fixative solutions used in human gross anatomy: A mice brain immunohistochemistry study

Antigenicity is preserved with fixative solutions used in human gross anatomy: A mice brain immunohistochemistry study

Consumer perceptions of edible packaging made of gelatin as chili powder packaging

Hair Removal Potential of Keratinase Produced by Bacillus Species Isolated From Feather Waste

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