Current Insights on Vegetative Insecticidal Proteins (Vip) as Next Generation Pest Killers

Syed, Tahira; Askari, Muhammad; Meng, Zhigang; Li, Yanyan; Abid, Muhammad Ali; Wei, Yunxiao; Guo, Sandui; Liang, Chengzhen; Zhang, Rui

doi:10.3390/toxins12080522

Cited by 46 publications

(23 citation statements)

References 118 publications

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“…Vip3 proteins are toxic to some lepidopteran larvae and have striking structural homology to Cry toxins, despite sharing no primary sequence similarity [ 27 ]. Like Cry toxins, Vip3 toxins are thought to kill insects by binding to specific receptors in the midgut [ 27 , 28 ]. Because Vip3 and Cry toxins do not bind to the same midgut receptors, strong cross-resistance between them is unlikely [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

Early Warning of Resistance to Bt Toxin Vip3Aa in Helicoverpa zea

Yang

Kerns

Little

et al. 2021

Toxins

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Evolution of resistance by pests can reduce the benefits of crops genetically engineered to produce insecticidal proteins from Bacillus thuringiensis (Bt). Because of the widespread resistance of Helicoverpa zea to crystalline (Cry) Bt toxins in the United States, the vegetative insecticidal protein Vip3Aa is the only Bt toxin produced by Bt corn and cotton that remains effective against some populations of this polyphagous lepidopteran pest. Here we evaluated H. zea resistance to Vip3Aa using diet bioassays to test 42,218 larvae from three lab strains and 71 strains derived from the field during 2016 to 2020 in Arkansas, Louisiana, Mississippi, Tennessee, and Texas. Relative to the least susceptible of the three lab strains tested (BZ), susceptibility to Vip3Aa of the field-derived strains decreased significantly from 2016 to 2020. Relative to another lab strain (TM), 7 of 16 strains derived from the field in 2019 were significantly resistant to Vip3Aa, with up to 13-fold resistance. Susceptibility to Vip3Aa was significantly lower for strains derived from Vip3Aa plants than non-Vip3Aa plants, providing direct evidence of resistance evolving in response to selection by Vip3Aa plants in the field. Together with previously reported data, the results here convey an early warning of field-evolved resistance to Vip3Aa in H. zea that supports calls for urgent action to preserve the efficacy of this toxin.

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Section: Introductionmentioning

confidence: 99%

Early Warning of Resistance to Bt Toxin Vip3Aa in Helicoverpa zea

Yang

Kerns

Little

et al. 2021

Toxins

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“…Several SMs such as phloroglucinols, dialkylresorcinols, phenazines, pyoluteorine, and pyrrolnitrin that are lipopeptides extracted from Pseudomonas spp., exhibiting a wide range of antibacterial and antifungal activities have been characterized [57]. Crystal proteins, vegetative insecticidal proteins, d-endotoxins, and cytolytic proteins produced by B. thuringiensis strains have been used against Lepidoptera, Diptera, Coleoptera, Hymenoptera, and Hemiptera species [58,59]. Prodigiosin extracted from Serratia marcescens is an effective SM at low concentrations for the control of nematode pests such as juveniles of Meloidogyne incognita [60].…”

Section: Next-generation Pesticidesmentioning

confidence: 99%

Are insect GPCRs ideal next‐generation pesticides: opportunities and challenges

et al. 2021

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The increasing human population, combined with low inefficiency and adverse effects of available pesticides, has magnified the urgent need of developing next-generation pesticides. Among the available approaches, strategies targeting invertebrate G protein-coupled receptors (GPCRs) are very promising as these receptors are the targets of endogenous neuropeptides/neuromodulators that upon binding to their receptors induce profound changes in insect physiology. Therefore, exploring GPCR regulators has great potential in the development of targeted next-generation pesticides. Despite the great potential of such alternative pesticides, so far there has been only one approved compound, Amitraz, which conveys its antipest activity via the GPCR Octopamine receptor. Here, we review the current status of pesticide development, hazards associated with conventional pesticide compounds, alternative strategies that involve next-generation of pesticides, structural features of GPCRs, and opportunities and challenges of targeting the members of this superfamily in invertebrates to develop anti-pest agents. In conclusion, we emphasize that the potential of GPCRs cannot be utilized in full without more genomic and transcriptomic data to improve our understanding of the complex network of peptidergic signaling pathways. We argue how vital it is to obtain three-dimensional (3D) structural data on physiologically important target GPCRs and encourage the readers to use the state of the art in silico methods such as virtual screening for the discovery of new pesticide compounds.

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“…There have been >150 Vip proteins belonging to four subfamilies reported so far. Of them, only Vip3 is represented by PFTs and is the largest group with 111 members [ 24 ]. Pyramiding Vip3Aa with Cry toxins appears to delay the development of field-evolved resistance against Bt -crops that express Cry proteins alone [ 25 ].…”

Section: Pore-forming Toxinsmentioning

confidence: 99%

Microbial Toxins in Insect and Nematode Pest Biocontrol

Subbaiah

2021

IJMS

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Invertebrate pests, such as insects and nematodes, not only cause or transmit human and livestock diseases but also impose serious crop losses by direct injury as well as vectoring pathogenic microbes. The damage is global but greater in developing countries, where human health and food security are more at risk. Although synthetic pesticides have been in use, biological control measures offer advantages via their biodegradability, environmental safety and precise targeting. This is amply demonstrated by the successful and widespread use of Bacillusthuringiensis to control mosquitos and many plant pests, the latter by the transgenic expression of insecticidal proteins from B. thuringiensis in crop plants. Here, I discuss the prospects of using bacterial and fungal toxins for pest control, including the molecular basis of their biocidal activity.

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Current Insights on Vegetative Insecticidal Proteins (Vip) as Next Generation Pest Killers

Cited by 46 publications

References 118 publications

Early Warning of Resistance to Bt Toxin Vip3Aa in Helicoverpa zea

Early Warning of Resistance to Bt Toxin Vip3Aa in Helicoverpa zea

Are insect GPCRs ideal next‐generation pesticides: opportunities and challenges

Microbial Toxins in Insect and Nematode Pest Biocontrol

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