Current knowledge on exocrine glands in carabid beetles: structure, function and chemical compounds

Giglio, Anita; Brandmayr, Pietro; Talarico, Federica; Brandmayr, Tullia Zetto

doi:10.3897/zookeys.100.1527

Cited by 29 publications

(18 citation statements)

References 55 publications

(63 reference statements)

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“…Through lineage tracing, we have previously determined the Drosophila spermathecal lineage with single-cell resolution ( Figure 1A ); however, it is unknown how each cell in this lineage is specified. In addition, the molecular mechanisms involved in other class-III secretory unit formations are unknown, despite their essential roles for insect physiology and behavior, including reproduction, digestion, defensive behavior, and social communication ( Sreng 2006 ; Billen 2011 ; Giglio et al 2011 ; Gomez‐Diaz and Benton 2013 ). Inspired by the enormous body of work on Notch in binary cell fate determination in the Drosophila sensory lineage ( Lai and Orgogozo 2004 ; Schweisguth 2015 ), we investigated its role in the spermathecal lineage.…”

Section: Discussionmentioning

confidence: 99%

Dynamic Notch Signaling Specifies Each Cell Fate inDrosophilaSpermathecal Lineage

Shen

Sun

2017

G3 Genes|Genomes|Genetics

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Spermathecae are glandular organs in the insect female reproductive tract that play essential roles in insect reproduction; however, the molecular mechanism involved in their development is largely unknown. Drosophila spermathecae consist of class-III secretory units, in which each secretory cell (SC) discharges its products to the central lumen through an end-apparatus and a canal. Secretory unit formation in Drosophila spermathecae utilizes a fixed cell lineage, in which each secretory unit precursor (SUP) divides to produce one pIIb cell and one pIIa cell. The former differentiates into an apical cell (AC), whereas the latter divides again to produce an SC and a basal cell (BC). It is unclear how each cell acquires its identity and contributes to secretory unit formation. Here, we demonstrate that Notch signaling is required and sufficient for the specification of lumen epithelial precursors (LEPs; vs. SUPs), pIIb (vs. pIIa), and SCs (vs. BCs) sequentially. To our surprise, Notch activation in LEPs and SCs apparently utilizes different ligand mechanisms. In addition, Notch signaling both suppresses and activates transcription factors Hindsight (Hnt) and Cut during spermathecal lineage specification, supporting the notion that Notch signaling can have opposite biological outcomes in different cellular environments. Furthermore, LEP-derived epithelial cells (ECs) and ACs show distinct cellular morphology and are essential for securing secretory units to the epithelial lumen. Our work demonstrates, for the first time, the dynamic role of Notch signaling in binary cell fate determination in Drosophila spermathecae and the role of ECs and ACs in secretory unit formation. KEYWORDSclass-III secretory gland spermathecae Notch signaling binary cell fate determination Cut Spermathecae are sperm-storage organs found in the female reproductive tract of many insect species, and they are important for reproduction and cryptic female choice (Eberhard 1996;Pitnick et al. 1999;Manier et al. 2010). Studies in Drosophila have shown that glandular secretions from spermathecae and parovaria act to attract, nourish, and protect sperm by creating an appropriate environment (Filosi and Perotti 1975;Allen and Spradling 2008;Prokupek et al. 2008Prokupek et al. , 2009Schnakenberg et al. 2011;Sun and Spradling 2013). This is likely true in other insect species (Shaw et al. 2014). In addition, glandular secretions from spermathecae and parovaria regulate ovulation and egg laying (Schnakenberg et al. 2011;Sun and Spradling 2013;Cattenoz et al. 2016). Although the exact identities of the secreted products regulating sperm and ovulation are unknown, it is clear that secretions through the canonical protein secretory pathway are required for sperm storage but not ovulation (Sun and Spradling 2013). Despite recent progress on the physiology of spermatheca secretion, the molecular mechanisms involved in spermathecal gland formation are largely unknown.Spermathecae in Drosophila melanogaster are a pair of mushroomshaped organs with a h...

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Section: Discussionmentioning

confidence: 99%

Dynamic Notch Signaling Specifies Each Cell Fate inDrosophilaSpermathecal Lineage

Shen

Sun

2017

G3 Genes|Genomes|Genetics

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“…The chemical compounds of the pygidial glands of Carabidae vary greatly (e.g. Giglio et al, 2011). Explosive mechanisms for delivery of compounds have evolved independently in Metriinae and Paussinae on the one hand, and in Brachininae on the other.…”

Section: Carabidaementioning

confidence: 99%

The morphological evolution of the Adephaga (Coleoptera)

Beutel

Ribera

Fikáček

et al. 2019

Systematic Entomology

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The evolution of the coleopteran suborder Adephaga is discussed based on a robust phylogenetic background. Analyses of morphological characters yield results nearly identical to recent molecular phylogenies, with the highly specialized Gyrinidae placed as sister to the remaining families, which form two large, reciprocally monophyletic subunits, the aquatic Haliplidae + Dytiscoidea (Meruidae, Noteridae, Aspidytidae, Amphizoidae, Hygrobiidae, Dytiscidae) on one hand, and the terrestrial Geadephaga (Trachypachidae + Carabidae) on the other. The ancestral habitat of Adephaga, either terrestrial or aquatic, remains ambiguous. The former option would imply two or three independent invasions of aquatic habitats, with very different structural adaptations in larvae of Gyrinidae, Haliplidae and Dytiscoidea.

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“…The pygidial gland secretions of ground beetles are chiefly composed of organic acids, aldehydes, alcohols, and terpenes (Eisner et al 1977;Moore 1979;Dazzini-Valcurone and Pavan 1980;Dettner 1987;Giglio et al 2011). Previous investigators determined the chemical composition of pygidial gland secretions of the ground beetle species Carabus ullrichii Germar, 1824; C. coriaceus L., 1758; Abax parallelepipedus (Piller & Mitterpacher, 1783); and Laemostenus punctatus (Dejean, 1828) (Lečić et al 2014;Vesović et al 2015Vesović et al , 2017.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of tumour and non-tumour cell proliferation by pygidial gland secretions of four ground beetle species (Coleoptera: Carabidae)

et al. 2018

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Inhibition of the proliferation of human tumour cells and porcine non-tumour cells by the pygidial gland secretion released by adults of four ground beetle species was observed in this study. The sulphorhodamine B (SRB) assay was applied to establish the percentages of inhibition of the net growth of four human tumour cell lines and porcine liver primary non-tumour cells. The secretions of all tested ground beetle species were shown to have an antiproliferative effect on the tested cell lines. Special emphasis is put on the secretion of Abax parallelepipedus, which showed the highest antitumour potential and weakest inhibition of non-tumour cell proliferation. The antitumour and antiproliferative potential of the pygidial gland secretions of ground beetles is here demonstrated for the first time. It is suggested that certain organic acids are responsible for the action. Further investigation needs to be conducted in order to better understand the mechanisms governing the observed cytotoxic and antitumour activity.

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Current knowledge on exocrine glands in carabid beetles: structure, function and chemical compounds

Cited by 29 publications

References 55 publications

Dynamic Notch Signaling Specifies Each Cell Fate inDrosophilaSpermathecal Lineage

Dynamic Notch Signaling Specifies Each Cell Fate inDrosophilaSpermathecal Lineage

The morphological evolution of the Adephaga (Coleoptera)

Inhibition of tumour and non-tumour cell proliferation by pygidial gland secretions of four ground beetle species (Coleoptera: Carabidae)

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