Current methods and prospects of coronavirus detection

Bu, Jiaqi; Deng, Zhiwei; Liu, Hui; Li, Jiacheng; De, Wang; Yang, Yanjing; Zhong, Shian

doi:10.1016/j.talanta.2020.121977

Cited by 17 publications

(9 citation statements)

References 197 publications

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“…The SARS-Cov-2 virus belongs to beta-Cov lineage B RNA viruses. It is characterized by apparent stick protrusion [ 212 , 213 ] on the virus's surface and an abnormally large RNA genome [ 214 ]. SARS-Cov-2 virus genome encodes spike (S) protein, matrix protein (M) nucleocapsid (N) protein, and envelope (E) proteins.…”

Section: Molecular and Non-molecular Virus Detection Techniquesmentioning

confidence: 99%

Advances and insights in the diagnosis of viral infections

2021

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Viral infections are the most common among diseases that globally require around 60 percent of medical care. However, in the heat of the pandemic, there was a lack of medical equipment and inpatient facilities to provide all patients with viral infections. The detection of viral infections is possible in three general ways such as (i) direct virus detection, which is performed immediately 1–3 days after the infection, (ii) determination of antibodies against some virus proteins mainly observed during/after virus incubation period, (iii) detection of virus-induced disease when specific tissue changes in the organism. This review surveys some global pandemics from 1889 to 2020, virus types, which induced these pandemics, and symptoms of some viral diseases. Non-analytical methods such as radiology and microscopy also are overviewed. This review overlooks molecular analysis methods such as nucleic acid amplification, antibody-antigen complex determination, CRISPR-Cas system-based viral genome determination methods. Methods widely used in the certificated diagnostic laboratory for SARS-CoV-2, Influenza A, B, C, HIV, and other viruses during a viral pandemic are outlined. A comprehensive overview of molecular analytical methods has shown that the assay's sensitivity, accuracy, and suitability for virus detection depends on the choice of the number of regions in the viral open reading frame (ORF) genome sequence and the validity of the selected analytical method.

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Section: Molecular and Non-molecular Virus Detection Techniquesmentioning

confidence: 99%

Advances and insights in the diagnosis of viral infections

2021

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“…Generally, a biosensor [42,[127][128][129][130][131][132] consists of two parts: the recognition probe and the transducer substrate. The recognition probe should identify and bind target analytes with the highest possible selectively and the transducer should transform the biometric events into physical signals, with a high amplification ratio to be received and analyzed.…”

Section: Functionalization Of Nps By Aryldiazonium Salts For Nano-biosensorsmentioning

confidence: 99%

Surface functionalization of nanomaterials by aryl diazonium salts for biomedical sciences

Luo

Onidas

et al. 2021

Advances in Colloid and Interface Science

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Nanoparticles (NPs) can be prepared by simple reactions and methods from a number of materials. Their small size opens up a number of applications in different fields, among which biomedicine, including: i) drug delivery, ii) biosensors, iii) bioimaging, iv) antibacterial activity. To be able to perform such tasks, NPs must be modified with a variety of functional molecules, such as drugs, targeting groups, chemical tags or antibacterial agents, and must also be prevented from aggregation. The attachment must be stable to resist during the transportation to the targeted location. Diazonium salts, which have been widely used for coupling applications and surface modification, fulfil such criteria. Moreover, they are simple to prepare and can be easily substituted with a large number of organic groups. This review describes the use of these compounds in nanomedicine with a focus on the construction of nanohybrids derived from metal, oxide and carbon-based NPs as well as viruses.

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“…Methodologies for virus detection are continuously evolving, as noticed in several reviews and original research [22][23][24][25]. These methods vary widely, from the biological tests, which are time-demanding but detect infectious viruses, to the serological and molecular techniques that provide faster analysis, but without discriminating between infectious and noninfectious particles [3,12,26].…”

Section: Virus Detectionmentioning

confidence: 99%

“…ELISA is more sensitive than microscopy, relying on the antigen (virus) capture (direct or indirect) and immobilization of the antigen-antibody complex on solid supports (polystyrene), followed by a washing step to remove the matrix substances and then detection. In the so-called Sandwich ELISA, a capturing antibody layer immobilized in the plates retains the antigens, followed by washing and binding to a labeled detection-antibody conjugated to horseradish peroxidase, alkaline phosphatase, or biotin [3,21,22]. In the virus presence, the detectionconjugate antibody remains in the plate after the washing and changes the color or luminesces upon treatment with specific reagents.…”

Section: Virus Detectionmentioning

confidence: 99%

Polymer monoliths for the concentration of viruses from environmental waters: A review

Vitek

Nascimento

Masini

2021

J of Separation Science

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Even at low concentrations in environmental waters, some viruses are highly infective, making them a threat to human health. They are the leading cause of waterborne enteric diseases. In agriculture, plant viruses in irrigation and runoff water threat the crops. The low concentrations pose a challenge to early contamination detection. Thus, concentrating the virus particles into a small volume may be mandatory to achieve reliable detection in molecular techniques. This paper reviews the organic monoliths developments and their applications to concentrate virus particles from waters (waste, surface, tap, sea, and irrigation waters).Free-radical polymerization and polyaddition reactions are the most common strategies to prepare the monoliths currently used for virus concentration. Here, the routes for preparing and functionalizing both methacrylate and epoxy-based monoliths will be shortly described, following a revision of their retention mechanisms and applications in the concentration of enteric and plant viruses in several kinds of waters.

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Current methods and prospects of coronavirus detection

Cited by 17 publications

References 197 publications

Advances and insights in the diagnosis of viral infections

Advances and insights in the diagnosis of viral infections

Surface functionalization of nanomaterials by aryl diazonium salts for biomedical sciences

Polymer monoliths for the concentration of viruses from environmental waters: A review

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