Current opinion and perspectives on the methods for tracking and monitoring plant growth‒promoting bacteria

Rilling, Joaquín I.; Acuña, Jacquelinne J.; Nannipieri, Paolo; Cassán, Fabricio; Maruyama, Fumito; Jorquera, Milko A.

doi:10.1016/j.soilbio.2018.12.012

Cited by 114 publications

(86 citation statements)

References 127 publications

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“…Most studies published to date do not include monitoring of PGPR in the environment after their application and mostly rely on measuring plant biomass and physiology‐related parameters to confirm PGPR activity without properly confirming bacterial root colonization (Rilling et al . ). However, one of the most important traits of a successful PGPR is its ability to colonize the rhizosphere (Compant et al .…”

Section: Discussionmentioning

confidence: 97%

“…; Beauregard ; Rilling et al . ). The colonization process is highly regulated, from initial migration into the root zone to the formation of bacterial biofilms (Chin‐A‐Woeng et al .…”

Section: Discussionmentioning

confidence: 97%

“…Our results showed that the developed qPCR assay was able to provide valuable and reliable information regarding the colonization of LBUM677 in the rhizosphere. Most studies published to date do not include monitoring of PGPR in the environment after their application and mostly rely on measuring plant biomass and physiology-related parameters to confirm PGPR activity without properly confirming bacterial root colonization (Rilling et al 2019). However, one of the most important traits of a successful PGPR is its ability to colonize the rhizosphere (Compant et al 2005;Beauregard 2015;Rilling et al 2019).…”

Section: Discussionmentioning

confidence: 99%

“…Most studies published to date do not include monitoring of PGPR in the environment after their application and mostly rely on measuring plant biomass and physiology-related parameters to confirm PGPR activity without properly confirming bacterial root colonization (Rilling et al 2019). However, one of the most important traits of a successful PGPR is its ability to colonize the rhizosphere (Compant et al 2005;Beauregard 2015;Rilling et al 2019). The colonization process is highly regulated, from initial migration into the root zone to the formation of bacterial biofilms (Chin-A-Woeng et al 1997).…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Persistence ofPseudomonas fluorescensLBUM677 in the rhizosphere of corn gromwell (Buglossoides arvensis) under field conditions and its impact on seed oil and stearidonic acid bioaccumulation

Novinscak

Filion

2019

J Appl Microbiol

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Aims The aim of this study was to evaluate the persistence of Pseudomonas fluorescens LBUM677 in the rhizosphere of Buglossoides arvensis under agricultural field conditions and determine if B. arvensis intraspecific genetic variations affect the capacity of LBUM677 to colonize its rhizosphere and increase seed oil and stearidonic acid (SDA) accumulation. Methods and Results Two field experiments were performed to: (i) study the persistence of various concentrations of LBUM677 inoculated in the rhizosphere of B. arvensis and determine a minimum inoculation threshold required to maximize biological activity; and (ii) study the impact of B. arvensis intraspecific genetic variations on LBUM677 rhizosphere colonization and seed oil and SDA accumulation. In order to track LBUM677 populations in soil over time, a specific quantitative polymerase chain reaction assay was developed. Inoculation with a minimum of 109 LBUM677 bacterial cells per plant was determined as a threshold to promote maximum B. arvensis rhizosphere colonization and seed oil and SDA accumulation. Buglossoides arvensis intraspecific genetic variations had an impact on rhizosphere colonization, B. arvensis seed oil and SDA accumulation, where two cultivars benefited more than others from LBUM677 inoculation. Conclusions LBUM677 can colonize the rhizosphere and increase seed oil and SDA yields in B. arvensis plants in a cultivar‐dependant manner. Significance and Impact of the Study LBUM677 shows potential to be used as a biofertilizer to specifically increase seed oil and SDA yields in B. arvensis. This will in turn promote the development of an economically viable agricultural‐based approach as an alternative for producing high‐quality polyunsaturated fatty acids.

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Section: Discussionmentioning

confidence: 97%

“…; Beauregard ; Rilling et al . ). The colonization process is highly regulated, from initial migration into the root zone to the formation of bacterial biofilms (Chin‐A‐Woeng et al .…”

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Persistence ofPseudomonas fluorescensLBUM677 in the rhizosphere of corn gromwell (Buglossoides arvensis) under field conditions and its impact on seed oil and stearidonic acid bioaccumulation

Novinscak

Filion

2019

J Appl Microbiol

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“…Measuring the persistence of microbial inoculants in soil poses technical difficulties, as the inoculant needs to be identified from within a complex community. The tracking and monitoring of the persistence of PGPM released in the environment have been widely studied (Brandt and Kluepfel, 1991;Kloepper and Beauchamp, 1992;Stahl and Kane, 1992;Gamalero et al, 2003;Podile and Kishore, 2006;Ahmad et al, 2011;Glick, 2015;Rilling et al, 2019) to understand their behavior in soil and which factors influence their survival under various conditions. Several sets of techniques are currently used to detect root colonization and persistence in the soils: microbial enumerations by culture-based methods, microscopy-based techniques, and DNA-based methods.…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of Plant Beneficial Microbes: Overview of the Methodological Approaches for the Assessment of Root Colonization and Persistence

2020

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Synergistic effects of substrate inoculation with Pseudomonas strains on tomato phenology, yield, and selected human health‐related phytochemical compounds

Issifu,

Naitchede,

Ateka

et al. 2023

Agrosystems Geosci & Env

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Plant growth‐promoting rhizobacteria enhances nutritional availability during root colonization. Pseudomonas is the most popular bioinoculant due to its synergistic effects on plant development. This study evaluated the impact of four Pseudomonas strain substrate inoculations on tomato phenology, yield, and selected phytochemical components relevant to human health. The bacterial isolates have been characterized morphologically, biochemically, physiologically, and phylogenetically. Tomato plants on cocopeat substrate were inoculated individually with a bacterial suspension (optical density [OD]600 2.0). Controls included nitrogen–phosphate–potassium (NPK) fertilizer and tap water. The greenhouse experiment was laid out in a completely randomized design with five replications. Data were taken 30, 60, 90, and 120 days after inoculation. All four bacterial isolates could fix nitrogen, solubilize phosphate, and potassium and synthesize indole acetic acid in vitro. The 16S rRNA of the strains BF1P2‐6, BF2P1‐2, BF2P5‐1, and BF4P2‐5 were identified as Pseudomonas putida Y22, Pseudomonas taiwanensis GDLB‐32, Pseudomonas monteilii Bil‐NOR3_15, and Pseudomonas fluorescens Y5, respectively. The strains grew best at pH 7, NaCl 0.0%, and 35°C. Inoculated tomato plants tested over a period of 4 months in a greenhouse showed a significant increase in growth, leaf variables, and yield attributes. Pseudomonas‐inoculated plants were the highest in tannins (46.96 mg/100 g), followed by lycopene (45.57 mg/100 g) and Carotenoids (31.87 mg/100 g). The antioxidant activity of tomato fruit extracts showed the potential for free radicals scavenging activity against 2, 2‐diphenyl‐1‐picrylhydrazyl. Conditions such as pH and electrical conductivity for the cocopeat substrate were within acceptable limits. Bacterial strains and NPK increased cocopeat nitrogen, phosphate, and potassium bioavailability. This study reveals that Pseudomonas strains improve plant growth, yield, and health‐related phytochemical substances in tomato fruits, which could broaden their application in fruit and vegetable crops.

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Current opinion and perspectives on the methods for tracking and monitoring plant growth‒promoting bacteria

Cited by 114 publications

References 127 publications

Persistence ofPseudomonas fluorescensLBUM677 in the rhizosphere of corn gromwell (Buglossoides arvensis) under field conditions and its impact on seed oil and stearidonic acid bioaccumulation

Persistence ofPseudomonas fluorescensLBUM677 in the rhizosphere of corn gromwell (Buglossoides arvensis) under field conditions and its impact on seed oil and stearidonic acid bioaccumulation

Effectiveness of Plant Beneficial Microbes: Overview of the Methodological Approaches for the Assessment of Root Colonization and Persistence

Synergistic effects of substrate inoculation with Pseudomonas strains on tomato phenology, yield, and selected human health‐related phytochemical compounds

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