Current overview on challenges in regenerative endodontics

Abstract

Introduction:Regenerative endodontics provides hope of converting the non-vital tooth into vital once again. It focuses on substituting traumatized and pathological pulp with functional pulp tissue. Current regenerative procedures successfully produce root development but still fail to re-establish real pulp tissue and give unpredictable results. There are several drawbacks that need to be addressed to improve the quality and efficiency of the treatment.Aim:The aim of this review article is to discuss major priorities that ought to be dealt before applications of regenerative endodontics flourish the clinical practice.Materials and Methods:A web-based research on MEDLINE was done using filter terms Review, published in the last 10 years and Dental journals. Keywords used for research were “regenerative endodontics,” “dental stem cells,” “growth factor regeneration,” “scaffolds,” and “challenges in regeneration.” This review article screened about 150 articles and then the relevant information was compiled.Results:Inspite of the impressive growth in regenerative endodontic field, there are certain loopholes in the existing treatment protocols that might sometimes result in undesired and unpredictable outcomes.Conclusion:Considerable research and development efforts are required to improve and update existing regenerative endodontic strategies to make it an effective, safe, and biological mode to save teeth.

Cryopreservation and Banking of Dental Stem Cells

“…DSCs have gained great interest during the last decade because of their accessibility, (trans)differentiation potential and high proliferative capacity. Elaborate preclinical research has highlighted the underlying biological and cellular properties of different DSCs, as well as their potential application in the treatment of medical conditions [92][93][94][95][96][97], such as myocardial infarction [98], ischemic disease [99], nerve injury and other neurodegenerative disorders [70,47,[100][101][102][103][104][105]50], inflammatory diseases [106,107], diabetes [108][109][110][111][112], muscular dystrophy [113,26,114], bone/cartilage defects [115][116][117][118][119], hair follicle loss [120], skin injuries [121], salivary gland defects [122], corneal epithelial defects [123][124][125][126], and the regeneration of dental tissues [127][128][129][130]…”

“…The main applications for DSCs are in the regeneration of damaged tooth-related structures such as dentin, pulp and periodontal ligament [128][129][130]15,[131][132][133][134][135]. A significant number of studies in animal models have accomplished regeneration of dentin/pulp tissue and of the cementum/periodontal complex by applying DSCs in combination with an appropriate scaffold or supporting matrix [5,7,136,134,137].…”

Applications of regenerative medicine in organ transplantation

“…[ 75 ] Of all these, only root canal revascularization approach is clinically feasible while rest others exist in research fields. [ 76 ] With new discoveries, innovative ideas, and high-quality research, regenerative therapies have the potential to revolutionize dentistry.…”

Regenerative Applications Using Tooth Derived Stem Cells in Other Than Tooth Regeneration: A Literature Review

“…The majority of research employing dental stem cells has been directed towards regeneration of damaged tooth related structures, either partially or in its entirety [ 33 ]. Indeed, the findings of a substantial number of studies have garnered increasing interest in dental pulp regeneration [ 34 , 35 ], that is, regenerative endodontic procedures that may be able to change the fundamental paradigm of endodontics [ 36 ].…”