Current peptidomics: Applications, purification, identification, quantification, and functional analysis

Abstract

Peptidomics is an emerging field branching from proteomics that targets endogenously produced protein fragments. Endogenous peptides are often functional within the body—and can be both beneficial and detrimental. This review covers the use of peptidomics in understanding digestion, and identifying functional peptides and biomarkers. Various techniques for peptide and glycopeptide extraction, both at analytical and preparative scales, and available options for peptide detection with MS are discussed. Current algorithms for peptide sequence determination, and both analytical and computational techniques for quantification are compared. Techniques for statistical analysis, sequence mapping, enzyme prediction, and peptide function, and structure prediction are explored.

Peptides: Production, bioactivity, functionality, and applications

“…Traditional identification approaches, which require the knowledge of the parent protein sequences, match tandem spectra with theoretical spectra derived from predicted peptides in a protein library (Espejo-Carpio et al, 2013;Gu and Wu, 2013;de Gobba et al, 2014b). Primary structure of proteins can be accessed from online databases such as UniProtKB/Swiss-Prot or NCBI and the identification process can be carried out by using database search engines (e.g., Mascot) (Dallas et al, 2015;Le-Maux et al, 2015). On the other hand, de novo sequencing approach does not require a protein library and deduces peptide amino acid sequence by calculating mass differences between fragments from the tandem mass spectra (Girgih et al, 2014;Garc ıa-Moreno et al, 2015).…”

Peptidomic Analysis of Cultured Cardiomyocytes Exposed to Acute Ischemic-Hypoxia

“…Peptidomics is an emerging branch of proteomics that has attracted wide attention in recent years [3]. Peptidomics attempts to perform a quantitative and qualitative analysis of peptides in a given cell type, tissue or disease state.…”

Potential Significance of Peptidome in Human Ovarian Cancer for Patients With Ascites

“…Peptidomics, as an emerging branch of proteomics that aimed for producing protein fragments, 18 could be used to screen for amounts of disease biomarkers from serum, amniotic fluid, and urine. 19Y21 As was shown in previously mentioned literatures, peptides were closely linked to a variety of biological processes and functions.…”