Current practice patterns of rectal suction biopsy in the diagnostic work-up of Hirschsprung’s disease: results from an international survey

Friedmacher, Florian; Puri, Prem

doi:10.1007/s00383-016-3907-0

Cited by 31 publications

(22 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…6 Another recent questionnaire distributed to participants and faculty members at the 21st International Meeting of the Pediatric Colorectal Society (78% staff surgeons representing 30 countries) found that 15% of respondents prefer open fullthickness biopsy under general anesthesia for the evaluation of suspected Hirschsprung's disease. 4 Among those surgeons using SRB for the diagnosis, there was a wide variety in the device used as well as the technique of obtaining tissue samples. A median of 10% (range, 0-40%) of biopsies were reported to be insufficient requiring a repeat SRB or open full-thickness biopsy.…”

Section: Discussionmentioning

confidence: 99%

“…3 Suction rectal biopsy (SRB) has been considered the gold standard for the diagnosis of Hirschsprung's disease. 4 However, a recent study conducted in the Netherlands questioned the accuracy of SRB, especially regarding its utility in neonates. The authors recommended delaying rectal biopsy until at least 39 days of age due to a false-negative rate of 20.7% in infants younger than this.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Accuracy of Suction Rectal Biopsy for Diagnosis of Hirschsprung's Disease in Neonates

et al. 2018

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Accuracy of Suction Rectal Biopsy for Diagnosis of Hirschsprung's Disease in Neonates

et al. 2018

View full text Add to dashboard Cite

show abstract

“…In the same study, adequate submucosal tissue was absent in about half of the patients in the rectal suction biopsy group . In other studies, about 10–20% of rectal suction biopsies proved to be inadequate either due to small size or insufficient inclusion of submucosa . Therefore, endoscopic sampling using band‐EMR can be a useful alternative to the conventional biopsy techniques.…”

Section: Discussionmentioning

confidence: 79%

Diagnosis of Hirschsprung's disease in children: Preliminary evaluation of a novel endoscopic technique for rectal biopsy

et al. 2018

View full text Add to dashboard Cite

Background and AimThe diagnosis of Hirschsprung's disease (HD) relies on anorectal manometry and rectal biopsy. The role of endoscopic biopsy is uncertain for the diagnosis of HD in children. In this study, we evaluated the adequacy of biopsies procured by endoscopic mucosal resection (EMR) for the diagnosis of HD.MethodsConsecutive children with suspected HD from January 2013 to January 2018 were enrolled in the study. EMR was performed using the standard band ligation device at a distance of about 3 cm from dentate line in rectum. All samples were assessed macroscopically and microscopically. An adequate sample was defined as those measuring >3 mm and including adequate submucosa.ResultsA total of 132 children underwent evaluation for constipation in the study period. Of these, 10 children (median age, 4.25 years) underwent EMR using the band ligation device for the evaluation of HD. EMR was performed with and without submucosal lifting injection in four and six children, respectively. All the samples were adequate macroscopically (>3 mm). Absence of ganglion cells was noted in six children. Of these, three children underwent full‐thickness rectal biopsy followed by surgery. Three children did not undergo surgery. Ganglion cells were identified in four children, thereby excluding the diagnosis of HD.ConclusionRectal biopsy using EMR with a band ligation device is feasible, safe, and provides adequate sample for the evaluation of HD in children.

show abstract

“…These findings are compatible with previous studies [17, 18]. Although the diagnostic accuracy of HE staining is relatively lower than that of calretinin and AChE staining, a recent study has revealed that the most frequently used staining methods for rectal biopsies were HE (75.9%) and AChE (73.6%; sensitivity and specificity: 96.8% and 99.4%, respectively), followed by calretinin (33.3%; sensitivity and specificity: 99.1% and 100%, respectively) [7, 10, 19]. Moreover, the use of a variety of complimentary staining tools with HE helps to further increase the diagnostic accuracies by histology, including AChE, calretinin, and S100 [7, 9, 10, 19, 20].…”

Section: Discussionmentioning

confidence: 99%

“…While immunohistochemistry (IHC) methods have been widely used for the diagnosis of HSCR in developed countries [6], there are very few studies of their use in developing countries where hematoxylin and eosin (HE) staining is the important element of HSCR diagnosis [7]. …”

Section: Introductionmentioning

confidence: 99%

The utility of the hematoxylin and eosin staining in patients with suspected Hirschsprung disease

et al. 2017

View full text Add to dashboard Cite

BackgroundWhile immunohistochemistry (IHC) methods have been widely conducted for the diagnosis of Hirschsprung disease (HSCR) in developed countries, there are very few studies on their use in developing countries where hematoxylin and eosin (HE) staining is a key element of the diagnosis of HSCR. We aimed to determine the accuracy of HE staining in the diagnosis of HSCR using S100 IHC as the reference standard in Indonesia.MethodsAll histopathology performed for the suspicion of HSCR patients from January 2013 to August 2015 in Dr. Sardjito Hospital, Yogyakarta, Indonesia, were retrospectively reviewed.ResultsOur study included 23 HSCR patients: 9 males and 14 females. The HE staining revealed 14 negative (absence of ganglion cells) cases (61%) and 9 positive (presence of ganglion cells) cases (39%). In S100 IHC, out of the 9 positive cases by HE staining, 6 (67%) were confirmed for having ganglion cells; and out of the 14 negative cases by HE staining, 12 (86%) were reported negative and 2 (14%) were positive by S100 IHC staining. The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy rates of the HE staining were 80% (95% CI: 0.51–0.95), 75% (95% CI: 0.36–0.96), 85.7% (95% CI: 0.56–0.98), 66.7% (95% CI: 0.31–0.91), and 78.3% (95% CI: 0.58–0.90), respectively.ConclusionsOur study showed that HE staining has relatively moderate accuracy for the diagnosis of HSCR. The use of HE staining is still recommended for the diagnosis of HSCR given the limitation of resource allocation for more expensive IHC technologies in developing countries.

show abstract

Current practice patterns of rectal suction biopsy in the diagnostic work-up of Hirschsprung’s disease: results from an international survey

Cited by 31 publications

References 37 publications

Accuracy of Suction Rectal Biopsy for Diagnosis of Hirschsprung's Disease in Neonates

Accuracy of Suction Rectal Biopsy for Diagnosis of Hirschsprung's Disease in Neonates

Diagnosis of Hirschsprung's disease in children: Preliminary evaluation of a novel endoscopic technique for rectal biopsy

The utility of the hematoxylin and eosin staining in patients with suspected Hirschsprung disease

Contact Info

Product

Resources

About