Current progress in DNA barcoding and future implications for entomology

Jinbo, Utsugi; Kato, Tadahiro; Itô, Motomi

doi:10.1111/j.1479-8298.2011.00449.x

Cited by 240 publications

(215 citation statements)

References 174 publications

(285 reference statements)

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“…In the present study, we provided COI gene sequences of 16 mosquito species which were not available previously in sequence databases (Additional file 1). We believe that those new sequences would contribute to the on-going global effort to standardise DNA barcoding as a molecular means of species identification by the Consortium for the Barcode of Life (CBOL) [8]. Secondly, the cut-off limit of ?…”

Section: Discussionmentioning

confidence: 99%

DNA barcoding: complementing morphological identification of mosquito species in Singapore

Chan

Chiang²,

Hapuarachchi

et al. 2014

Parasites & Vectors

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Background: Taxonomy that utilizes morphological characteristics has been the gold standard method to identify mosquito species. However, morphological identification is challenging when the expertise is limited and external characters are damaged because of improper specimen handling. Therefore, we explored the applicability of mitochondrial cytochrome C oxidase subunit 1 (COI) gene-based DNA barcoding as an alternative tool to identify mosquito species. In the present study, we compared the morphological identification of mosquito specimens with their differentiation based on COI barcode, in order to establish a more reliable identification system for mosquito species found in Singapore. Methods: We analysed 128 adult mosquito specimens, belonging to 45 species of 13 genera. Phylogenetic trees were constructed for Aedes, Anopheles, Culex and other genera of mosquitoes and the distinctive clustering of different species was compared with their taxonomic identity. Results: The COI-based DNA barcoding achieved a 100% success rate in identifying the mosquito species. We also report COI barcode sequences of 16 mosquito species which were not available previously in sequence databases. Conclusions: Our study utilised for the first time DNA barcoding to identify mosquito species in Singapore. COI-based DNA barcoding is a useful tool to complement taxonomy-based identification of mosquito species.

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Section: Discussionmentioning

confidence: 99%

DNA barcoding: complementing morphological identification of mosquito species in Singapore

Chan

Chiang²,

Hapuarachchi

et al. 2014

Parasites & Vectors

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“…Since its inauguration in 2004, the Consortium for the Barcode of Life -CBOL, managed primarily by the Canadian Centre for DNA Barcoding at the Biodiversity Institute of Ontario, University of Guelph, Ontario, Canada, has gathered partners from all over the world. Their objective is to build, in less than twenty years, a comprehensive database that will include barcode sequences of all extant eukaryotes , Ratnasingham & Hebert 2007, Jinbo et al 2011.…”

Section: Dna Barcoding In Entomologymentioning

confidence: 99%

“…While the general interest in documenting species diversity has grown exponentially over the years, the number of taxonomists and other professionals trained in species identification, such as parataxonomists (Jinbo et al 2011), has steadily declined. Taking this scenario into account, several researchers have attempted to find different ways to accelerate and facilitate the process of species identification making it accessible to non-specialists.…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial pseudogenes in insect DNA barcoding: differing points of view on the same issue

Leite

2012

Biota Neotrop.

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Molecular tools have been used in taxonomy for the purpose of identification and classification of living organisms. Among these, a short sequence of the mitochondrial DNA, popularly known as DNA barcoding, has become very popular. However, the usefulness and dependability of DNA barcodes have been recently questioned because mitochondrial pseudogenes, non-functional copies of the mitochondrial DNA incorporated into the nuclear genome, have been found in various taxa. When these paralogous sequences are amplified together with the mitochondrial DNA, they may go unnoticed and end up being analyzed as if they were orthologous sequences. In this contribution the different points of view regarding the implications of mitochondrial pseudogenes for entomology are reviewed and discussed. A discussion of the problem from a historical and conceptual perspective is presented as well as a discussion of strategies to keep these nuclear mtDNA copies out of sequence analyzes. Keywords: COI, molecular, NUMTs. LEITE, L.A.R. Pseudogenes mitocondriais no DNA barcoding em insetos: diferentes pontos de vista sobre a mesma questão. Biota Neotrop. 12(3): http://www.biotaneotropica.org.br/v12n3/pt/abstract?thematicreview+bn02412032012Resumo: Ferramentas moleculares têm sido utilizadas para os estudos referentes à identificação e classificação dos organismos vivos. Entre estes, uma curta sequência do DNA mitocondrial, popularmente conhecida como DNA barcoding, tornou-se muito popular. No entanto, a utilidade e confiabilidade dos códigos de barras de DNA têm sido recentemente questionadas porque pseudogenes mitocondriais, cópias não-funcionais do DNA mitocondrial incorporados ao genoma nuclear, foram encontrados em vários táxons. Quando estas sequências parálogas são amplificadas juntamente com o DNA mitocondrial, podem passar despercebidas e acabam sendo analisadas como se fossem seqüências ortólogas. Nesta contribuição objetivou-se revisar e discutir os diferentes pontos de vista sobre as implicações de pseudogenes mitocondriais para entomologia. Discutimos também o problema através de uma perspectiva histórica e conceitual, abordando estratégias para eliminar ou evitar a presença dessas cópias nucleares em meio às sequências funcionais de DNA. Palavras-chave: COI, molecular, NUMTs.

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“…Normally, searching and screening of control agents require long-term feeding experiments, while DNA barcoding allows to identify and select control agents based on their gut contents (Jinbo et al, 2011;Neumann et al, 2010), detecting prey DNA from the gut content or feces of the predators (Symondson, 2002;Valentini et al, 2008).…”

Section: Application Of Dna Barcoding In Crop Protectionmentioning

confidence: 99%

DNA Sequencing and Crop Protection

Tedeschi¹

2012

DNA Sequencing - Methods and Applications

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Current progress in DNA barcoding and future implications for entomology

Cited by 240 publications

References 174 publications

DNA barcoding: complementing morphological identification of mosquito species in Singapore

DNA barcoding: complementing morphological identification of mosquito species in Singapore

Mitochondrial pseudogenes in insect DNA barcoding: differing points of view on the same issue

DNA Sequencing and Crop Protection

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