Current Progress in Transcriptomics and Proteomics of Latex Physiology and Metabolism in the Hevea brasiliensis Rubber Tree

Li, Dejun; Wu, Shan; Dai, Longjun

doi:10.1007/978-3-030-42258-5_8

Cited by 3 publications

(3 citation statements)

References 87 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, a study involving RRIM 600 and RY 7-20-59 suggested that the higher natural rubber yield in RY 7-20-59 likely resulted from increased total IPP supply, more IPP distribution for rubber biosynthesis and up-regulation of the genes involved in rubber biosynthesis pathway (see Li et al, 2020 for details account of transcriptomics). The MYB family members (HblMYB19 and HblMYB44) in the laticifer cells of Hevea could increase the activity of the promoters HbFDPS1, HbSRPP and HRT1, which are associated with the biosynthesis of natural rubber (Wang et al, 2017).…”

Section: Transcriptome Analysismentioning

confidence: 99%

“…The rubber biosynthesis pathway genes were not seen directly influencing higher yield (Tang et al, 2013). However, a study involving RRIM 600 and RY 7‐20‐59 suggested that the higher natural rubber yield in RY 7‐20‐59 likely resulted from increased total IPP supply, more IPP distribution for rubber biosynthesis and up‐regulation of the genes involved in rubber biosynthesis pathway (Li et al, 2015) (see Li et al, 2020 for details account of transcriptomics).…”

Section: Markers Transcriptomes and Proteomesmentioning

confidence: 99%

See 1 more Smart Citation

Molecular markers to devise predictive models for juvenile selection in Hevea rubber

Priyadarshan

2022

Plant Breeding

View full text Add to dashboard Cite

Natural rubber is extracted from the Hevea tree, through shaving the bark. As a major raw material source for many industries especially the tyre sector, Hevea rubber clones are intensively cultivated in the tropics. The world production of Hevea rubber is almost 13.64 million metric tonnes, and the productivity is around 1200 kg ha−1. However, the field production achieved so far is still well below the theoretical yield estimated (7000–12,000 kg ha−1). Even though this is a calculated estimate, efforts can be kept live to achieve this target, because Hevea rubber is being increasingly cultivated under several suboptimal environments after the 1980s. In this era of climate change, newer biotic and abiotic stresses encounter Hevea rubber culture that demands new clones at definite intervals. Hence, like any other species, Hevea rubber breeding is a continuous process in quest of new clones achieved through exploiting new sources of genetic variation. Hevea rubber breeding is to produce high‐yielding clones with good timber value. First priority is to explore and exploit genetic variation in clones, half‐sib, full‐sib and illegitimate (open‐pollinated) seedlings, where dry rubber yield and wood volume are the target traits. Next is to maximize genetic gains per unit time, through understanding juvenile–mature correlations and to practice selection of high‐yielding genotypes at the juvenile stage. Hevea rubber had a long breeding cycle of 33 years but could be reduced to 17 years after culling non‐essential steps of deriving clones. However, this could be reduced to 10 years, if a DNA marker‐assisted selection system is applied to seedlings. This system must also ensure significant juvenile–mature correlations on yield. This is a formidable task because under given circumstances, such a correlation is almost absent. However, this can be turned into a reality through employing yield‐related DNA markers with genomic selection (GS). The impact of DNA markers linked with REF, SRPP, acetyl‐CoA acetyltransferase, HMGS, HMGR and JAZ need to be specifically evaluated. GS that aims to improve quantitative traits by using genome‐wide marker data without requiring identification of markers associated with quantitative trait loci (QTLs) of interest can be used in Hevea also. Subsequently, by a predictive model, genomic estimated breeding values (GEBVs) can be calculated for untested individuals from a ‘testing population’. GS captures the total additive genetic variance with genome‐wide marker coverage and effect estimates. Therefore, selection of an individual without phenotypic data can be performed based on the individual's predicted breeding value. This review makes an assessment of the molecular markers in Hevea rubber and aims to streamline an efficient selection system to be applied onto seedlings through GS that owes a significant correlation with the mature phase.

show abstract

Section: Transcriptome Analysismentioning

confidence: 99%

Section: Markers Transcriptomes and Proteomesmentioning

confidence: 99%

Molecular markers to devise predictive models for juvenile selection in Hevea rubber

Priyadarshan

2022

Plant Breeding

View full text Add to dashboard Cite

show abstract

“…TPD can be detected early by monitoring latex peroxidase activity and the inorganic phosphate (Pi) content (Tristama et al., 2019 ). There is evidence that TPD susceptibility is an intrinsic genetically inherited characteristic (Jacob, 1989 ; Yang & Fan, 1995 ) that can be induced by overexploitation, i.e., excessive tapping and/or ET overstimulation (Chrestin, 1989 ; Fan & Yang, 1984 ; Faridah et al., 1996 ; Li et al., 2020 ; Nie et al., 2016 ). Nevertheless, the TPD‐associated molecular mechanisms are still rather elusive.…”

Section: Introductionmentioning

confidence: 99%

Downregulation of HbFPS1 affects rubber biosynthesis of Hevea brasiliensis suffering from tapping panel dryness

et al. 2023

View full text Add to dashboard Cite

SUMMARY Tapping panel dryness (TPD) is a century‐old problem that has plagued the natural rubber production of Hevea brasiliensis. TPD may result from self‐protective mechanisms of H. brasiliensis in response to stresses such as excessive hormone stimulation and mechanical wounding (bark tapping). It has been hypothesized that TPD impairs rubber biosynthesis; however, the underlying mechanisms remain poorly understood. In the present study, we firstly verified that TPD‐affected rubber trees exhibited lower rubber biosynthesis activity and greater rubber molecular weight compared to healthy rubber trees. We then demonstrated that HbFPS1, a key gene of rubber biosynthesis, and its expression products were downregulated in the latex of TPD‐affected rubber trees, as revealed by transcriptome sequencing and iTRAQ‐based proteome analysis. We further discovered that the farnesyl diphosphate synthase HbFPS1 could be recruited to small rubber particles by HbSRPP1 through protein–protein interactions to catalyze farnesyl diphosphate (FPP) synthesis and facilitate rubber biosynthesis initiation. FPP content in the latex of TPD‐affected rubber trees was significantly decreased with the downregulation of HbFPS1, ultimately resulting in abnormal development of rubber particles, decreased rubber biosynthesis activity, and increased rubber molecular weight. Upstream regulator assays indicated that a novel regulator, MYB2‐like, may be an important regulator of downregulation of HbFPS1 in the latex of TPD‐affected rubber trees. Our findings not only provide new directions for studying the molecular events involved in rubber biosynthesis and TPD syndrome and contribute to rubber management strategies, but also broaden our knowledge of plant isoprenoid metabolism and its regulatory networks.

show abstract

Transcriptomic and Proteomic Integration Reveals Key Tapping-Responsive Factors for Natural Rubber Biosynthesis in the Rubber Tree Hevea brasiliensis

He,

Yang,

et al. 2024

Forests

View full text Add to dashboard Cite

Natural rubber is a crucial industrial material, and it is primarily harvested from the latex of the rubber tree Hevea brasiliensis by tapping the tree trunk. During the regular tapping process, mechanical damage seriously affects latex reproduction and rubber yield, but the molecular mechanisms on tapping stimulation remain unclear. In this study, we firstly determined the changed physiological markers on latex regeneration, overall latex yield, and latex flow time during the tapping process. Then, we combined proteomics and transcriptomics analyses of latex during tapping and identified 3940 differentially expressed genes (DEGs) and 193 differentially expressed proteins (DEPs). Among them, 773 DEGs and 120 DEPs displayed a persistent upregulation trend upon tapping. It is interesting that, in the detected transcription factors, basic helix-loop-helix (bHLH) family members occupied the highest proportion among all DEGs, and this trend was similarly observed in DEPs. Notably, 48 genes and 34 proteins related to natural rubber biosynthesis were identified, and most members of small rubber particle protein (SRPP) and rubber elongation factor (REF) showed a positive response to tapping stimulation. Among them, SRPP6 and REF5 showed significant and sustained upregulation at the gene and protein levels following tapping, indicating their pivotal roles for post-tapping rubber biosynthesis. Our results deepen the comprehension of the regulation mechanism underlying tapping and provide candidate genes and proteins for improving latex production in the Hevea rubber tree in future.

show abstract

Current Progress in Transcriptomics and Proteomics of Latex Physiology and Metabolism in the Hevea brasiliensis Rubber Tree

Cited by 3 publications

References 87 publications

Molecular markers to devise predictive models for juvenile selection in Hevea rubber

Molecular markers to devise predictive models for juvenile selection in Hevea rubber

Downregulation of HbFPS1 affects rubber biosynthesis of Hevea brasiliensis suffering from tapping panel dryness

Transcriptomic and Proteomic Integration Reveals Key Tapping-Responsive Factors for Natural Rubber Biosynthesis in the Rubber Tree Hevea brasiliensis

Contact Info

Product

Resources

About