Current Research Method in Transporter Study

Wang, Dianlei

doi:10.1007/978-981-13-7647-4_4

Cited by 9 publications

(8 citation statements)

References 158 publications

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“…Transporters play an important role in the ADME of drugs. Recently, various in vitro and in vivo methods have been established for studying transporter function and drug transporter function [9,27].…”

Section: Discussionmentioning

confidence: 99%

Prediction of Absorption, Distribution, Metabolism, and Excretion Activity of the Components of Madhuca Longifolia and Its Inhibiting Target Molecule

Panchal

Ankitha

et al. 2020

Asian J Pharm Clin Res

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Objectives: Madhuca longifolia is a versatile tropical tree mostly cultivated or harvested in the wild in South Asia for its edible flowers and oil seeds. Mahua trees are vegetatively propagated; they act as soil improvers, and also help in soil reclamation and erosion control. M. longifolia is a plant of great importance due to its scientifically proven uses such as antioxidant activity, immune suppression, and neuroprotective activity, which is because of the various chemical constituents present in different parts of the plant. The aim of our study is to analyze the absorption, distribution, metabolism, and excretion (ADME) properties and pathways analysis of active components of M. longifolia. Methods: The detailed study of these chemical constituents is done using PubChem and software’s such as Rasmol and Pymol. Swiss ADME was used to find out the ADME properties of the chemical constituents present in the plant. The pathway analysis was done using a literature survey and Swiss TargetPrediction. Results: The research has identify the potentially active compound from the plant with its inhibitory target protein. Conclusion: The ADME result demonstrates the potential pharmacological activity of the plant compound, which can be studied through in vivo model against its potential inhibitory target molecules.

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Section: Discussionmentioning

confidence: 99%

Prediction of Absorption, Distribution, Metabolism, and Excretion Activity of the Components of Madhuca Longifolia and Its Inhibiting Target Molecule

Panchal

Ankitha

et al. 2020

Asian J Pharm Clin Res

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“…In situ/ex vivo models comprise isolated tissues or organs in an external environment, including the intestine, lung, liver, brain, kidney, and so on [112]. They have the minimum alteration of natural conditions compared to in vitro assays, while avoiding the influence of other organs [113].…”

Section: In Situ/ex Vivo Modelsmentioning

confidence: 99%

Transporter-mediated Natural Product-Drug Interactions

Wang

Ding

et al. 2022

Planta Med

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The increasing use of natural products in the clinical practice has raised great concerns about the potential natural product-drug interactions (NDIs). Drug transporters mediate the transmembrane passage of a broad range of drugs and thus are important determinants for drug pharmacokinetics and pharmacodynamics. Generally, transporters can be divided into ATP binding cassette (ABC) family and solute carrier (SLC) family. Numerous natural products have been identified as inhibitors, substrates, inducers, and/or activators of drug transporters. This review article aims to provide a comprehensive summary of the recent progress on the research of NDIs, focusing on the main drug transporters, such as P-glycoprotein (P-gp), breast cancer resistance protein (BCRP), organic anion transporter 1 and 3 (OAT1/OAT3), organic anion-transporting polypeptide 1B1 and 1B3 (OATP1B1/OATP1B3), organic cation transporter 2 (OCT2), multidrug and toxin extrusion protein 1 and 2-K (MATE1/MATE2-K). Additionally, the challenges and strategies of studying NDIs are also discussed.

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“…Whereas membrane vesicular transport assay generally uses inside-out oriented membranes prepared from different cell lines expressing ABC (e.g., SF9 insect cells, HEK293 and MDCK cells) [ 88 ]. Compared to ATPase assays, vesicular assays have the upper hand as they can estimate the exact transport mechanism of a drug or inhibitor [ 89 ]. Van Staden and colleagues [ 82 ] investigated the drug candidates affecting transporter function and DILI using membrane vesicles prepared from hepatocytes containing a bile salt export pump, MRP.…”

Section: D Cell Culture Systems To Evaluate Adme-tox Screeningmentioning

confidence: 99%

Role of in vitro two-dimensional (2D) and three-dimensional (3D) cell culture systems for ADME-Tox screening in drug discovery and development: a comprehensive review

Chunduri¹,

Maddi²

2022

ADMET DMPK

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Drug discovery and development have become a very time-consuming and expensive process. Preclinical animal models have become the gold standard for studying drug pharmacokinetic and toxicity parameters. However, the involvement of a huge number of animal subjects and inter-species pathophysiological variations between animals and humans has provoked a lot of debate, particularly because of ethical concerns. Although many efforts are being established by biotech and pharmaceutical companies for screening new chemical entities in vitro before preclinical trials, failures during clinical trials are still involved. Currently, a large number of two- dimensional (2D) in vitro assays have been developed and are being developed by researchers for the screening of compounds. Although these assays are helpful in screening a huge library of compounds and have shown perception, there is a significant lack in predicting human Absorption, Distribution, Metabolism, Excretion and Toxicology (ADME-Tox). As a result, these assays cannot completely replace animal models. The recent inventions in three-dimensional (3D) cell culture-based assays like organoids and micro-physiological systems have shown great potential alternative tools for predicting the compound pharmacokinetic and pharmacodynamic fate in humans. In this comprehensive review, we have summarized some of the most commonly used 2D in vitro assays and emphasized the achievements in next-generation 3D cell culture-based systems for predicting the compound ADME-Tox.

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Current Research Method in Transporter Study

Cited by 9 publications

References 158 publications

Prediction of Absorption, Distribution, Metabolism, and Excretion Activity of the Components of Madhuca Longifolia and Its Inhibiting Target Molecule

Prediction of Absorption, Distribution, Metabolism, and Excretion Activity of the Components of Madhuca Longifolia and Its Inhibiting Target Molecule

Transporter-mediated Natural Product-Drug Interactions

Role of in vitro two-dimensional (2D) and three-dimensional (3D) cell culture systems for ADME-Tox screening in drug discovery and development: a comprehensive review

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