Current Scenario of Patent Act: Compulsory Licensing

Abstract

Objective: Screening of alpha-pinene on L-arginine induced acute pancreatitis model. Material and Methods: In this model the acute pancreatitis was determined at 24h by determination of pancreatic wet weight/body weight ratio, nitrate/nitrite levels, lipid peroxidation (thiobarbituric acid reactive substances (TBARS)], proinflammatory cytokines [tumor necrosis factor (TNF)-α, C-reactive proteins (CRP) and interleukin (IL)], pancreatic myeloperoxidase (MPO) activity and serum levels of amylase and lipase. Results: Administration of L-arginine had induced pancreatitis and this was characterized by pulmonary complication including altered pancreatic wet weight/body weight ratio, nitrate/nitrite levels, proinflammatory cytokines, TBARS, MPO and serum levels of amylase and lipase. Treatment with alpha-pinene (100 and 200mg/kg) dose significantly attenuated the L-arginine-induced pancreatitis and related complication. Conclusion: The histological findings proved the amelioration of pancreatic injury by alphapinene and biochemical parameters proved anti-inflammatory and antioxidant property of alpha-pinene.

Determination of Anti‐Parkinson Drug Pramipexole Using a Label‐free Biosensor and Evaluation of its Interaction with ds‐DNA

“…Reportedly, the presence of PPX in real samples has been detected by different analytical techniques including ultra-performance liquid chromatography with a mass spectrometer (UPLC/MS) [3], gas chromatography/mass spectrometer (GC-MS) [4], capillary electrophoresis [5], liquid chromatography/mass spectrometer (LC-MS) [6] and high-performance thin-layer chromatography [7].…”