Current scenario of <scp>RNAi</scp>‐based hemipteran control

Jain, Ritesh G.; Robinson, Karl E.; Asgari, Sassan; Mitter, Neena

doi:10.1002/ps.6153

Cited by 65 publications

(96 citation statements)

References 61 publications

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“…The resulting complex, called AP-2, interacts with clathrin to form a clathrin-coated vesicle, which fuses to an endosome. Before the fusion of the late endosome to a lysosome, dsRNA molecules are released into the cytosol, where they trigger the RNAi core machinery [31,34,35]. Thus, the endocytotic mechanism of dsRNA uptake may promote RNAi-mediated gene silencing in organisms.…”

Section: Challenges In Rnai-mediated Silencing For Pest Controlmentioning

confidence: 99%

“…Additionally, the delivery methods that involve dsRNA acquisition via ingestion are associated with the additional challenges of the availability of dsRNA or siRNA in GMOs at amounts sufficient to trigger RNAi-mediated silencing response [35]. In GMOs, majority of the dsRNA molecules delivered are siRNAs, which may affect the stability of the molecules inside the insect body, as smaller molecules are more easily degraded by nucleases in the midgut [27,[50][51][52].…”

Section: Dsrna Deliverymentioning

confidence: 99%

“…dsRNA injection enables the delivery of a precise amount of dsRNA directly into the target tissues or the hemolymph, consequently achieving a high level of gene silencing [17,56,61]. The first contact before cellular uptake is either the hemolymph or the gut content [31], and these body fluids may act as the main causes of dsRNA instability due to the enzymatic activity of double-stranded ribonucleases (dsRNases) and the physiological pH driving enzymatic activity in these fluids [31,35]. Additionally, the lack of functional RNAi machinery and availability of limited knowledge on dsRNA uptake, transport, and systemic propagation mechanisms contribute to this instability [35].…”

Section: Dsrna Stability and Efficiencymentioning

confidence: 99%

“…The first contact before cellular uptake is either the hemolymph or the gut content [31], and these body fluids may act as the main causes of dsRNA instability due to the enzymatic activity of double-stranded ribonucleases (dsRNases) and the physiological pH driving enzymatic activity in these fluids [31,35]. Additionally, the lack of functional RNAi machinery and availability of limited knowledge on dsRNA uptake, transport, and systemic propagation mechanisms contribute to this instability [35]. The ACP genome harbors three dsRNase homologs, and dsRNA degradation due to high pH and/or dsRNase action in the gut and salivary secretions has already been confirmed [15].…”

Section: Dsrna Stability and Efficiencymentioning

confidence: 99%

See 3 more Smart Citations

Advances and Challenges in RNA Interference Technology for Citrus Huanglongbing Vector Control

Marques¹,

Angelotti-Mendonça

Roberto

2021

Horticulturae

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Citrus species, including sweet oranges, grapefruits, pomelos, and lemons, are the most widely cultivated trees and consumed fruits worldwide. In citrus orchard management, the control of Huanglongbing (HLB) disease and its insect vector Diaphorina citri (Asian citrus psyllid, ACP) represents a major global challenge. Consumers have been increasingly pushing the citrus production chain toward a more sustainable system, including stringent measures to prevent the use of chemical pesticides. In recent years, biotechnological advances have offered safe and environmentally friendly alternatives for crop production. Technologies such as RNA interference (RNAi)-mediated gene silencing have emerged as innovative tools for agricultural pest management. Here, we provide an overview of RNAi as a promising approach for ACP control and discuss the associated challenges. Despite the availability of specific silencing sequences aimed at a target gene of the insect pest, the uptake of double-stranded RNA is limited in hemipteran insects. In this context, improved delivery methods, stability maintenance, and RNAi response are considered the factors contributing to the increased effectiveness of exogenous RNAi against hemipteran pests. These approaches can serve as potential tools for efficient ACP control.

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Section: Challenges In Rnai-mediated Silencing For Pest Controlmentioning

confidence: 99%

Section: Dsrna Deliverymentioning

confidence: 99%

Section: Dsrna Stability and Efficiencymentioning

confidence: 99%

Section: Dsrna Stability and Efficiencymentioning

confidence: 99%

See 2 more Smart Citations

Advances and Challenges in RNA Interference Technology for Citrus Huanglongbing Vector Control

Marques¹,

Angelotti-Mendonça

Roberto

2021

Horticulturae

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“…The RNAi mechanism is currently applied in controlling Agricultural pests. Recent advances in RNAi technology have drastically increased knowledge on the RNAi mechanism and gene knockdown in several insects (Lü et al 2019;Vogel et al 2019;Adeyinka et al 2020;Husain et al 2021;Jain et al 2021). Scientists are currently improving the dsRNA delivery mode because the internalization of dsRNA is vital for effective RNAi in insects.…”

Section: Introductionmentioning

confidence: 99%

A Protective dsRNA is Crucial for Optimum RNAi Gene Silencing in Chilo partellus

Adeyinka¹

2021

IJAB

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RNAi technology is currently employed as an alternate control measure for agricultural pests. However, the variability of RNAi efficiency in insect pests limits the extensive usage of this technology and demands identifying the best target gene for effective RNAi. Four different bacterially-expressed dsRNA and purified dsRNAs coated on artificial diet were fed to the larvae. The transcripts expression was analyzed at 5 days and 15 days post-exposure to various dsRNAs. In the larvae fed on bacterially-expressed dsRNA, knockdown percentages were 80 and 57% knockdown in Acetylcholinesterase transcript, 40 and 60% gene knockdown in Arginine kinase, 74 and 73% knockdown in Chymotrypsin, and 80 and 20% reduction in V-ATPase transcript expression. Overall, the mRNA knockdown percentages in the targeted genes were more pronounced at 5 days of exposure to bacterially-expressed crude dsRNA than 15 days of exposure. However, most purified dsRNAs rarely induce any significant knockdown except dsARG, which reduced the arginine kinase transcript by 40%. Our findings suggest that for optimum RNAi in C. partellus, the dsRNA must be protected from direct access with nucleases. © 2021 Friends Science Publishers

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Identification and expression patterns of somatic piRNAs and PIWI genes in Riptortus pedestris (Hemiptera: Alydidae)

Ren,

Dong,

et al. 2024

Arch Insect Biochem Physiol

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RNA interference (RNAi)‐based gene silencing is a feasible and sustainable technology for the management of hemipteran pests by double‐stranded RNA involvement, including small‐interfering RNA, microRNA, and Piwi‐interacting RNA (piRNA) pathways, that may help to decrease the usage of chemical insecticides. However, only a few data are available on the somatic piRNAs and their biogenesis genes in Riptortus pedestris, which serves as a significant pest of soybean (Glycine max). In this study, two family members of the PIWI gene were identified and characterized in R. pedestris, containing Argonaute3 (RpAgo3) and Aubergine (RpAub) genes with conserved protein domains, and their clusters were validated by phylogenetic analysis. In addition, they were widely expressed in all developmental stages of the whole body of R. pedestris and had lower expression levels in R. pedestris guts under different rearing conditions based on previous transcriptome sequencing. Furthermore, abundant clean reads were filtered to a total number of 45,998 piRNAs with uridine bias at the first nucleotide (nt) position and 26–32 nt in length by mapping onto the reference genome of R. pedestris according to our previous whole‐transcriptome sequencing. Finally, our data revealed that gut bacterial changes were significantly positively or negatively associated with differentially expressed piRNAs among the five comparison groups with Pearson correlation analysis. In conclusion, these findings paved new avenues for the application of RNAi‐based biopesticides for broad‐spectrum hemipteran pest control.

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Current scenario of RNAi‐based hemipteran control

Cited by 65 publications

References 61 publications

Advances and Challenges in RNA Interference Technology for Citrus Huanglongbing Vector Control

Advances and Challenges in RNA Interference Technology for Citrus Huanglongbing Vector Control

A Protective dsRNA is Crucial for Optimum RNAi Gene Silencing in Chilo partellus

Identification and expression patterns of somatic piRNAs and PIWI genes in Riptortus pedestris (Hemiptera: Alydidae)

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