Current Source Density Estimation Using Microelectrode Array Data from the Hippocampal Slice Preparation

Thin-film epidural microelectrode arrays for somatosensory and motor cortex mapping in rat

“…0.1-mV signal amplitude) can be detected at distances of up to 100 m (Egert et al, 2002), field potentials (ca. 2-mV signal amplitude) at a maximum of 250 m (Wheeler and Nowak, 1986). In view of these data, an inter-electrode spacing of 600-700 m as used in our multielectrode array should be more than sufficient to prevent the overlap of catchment areas.…”

“…Small distances can lead to overlapping catchment areas, so that signals from single cellular sources are recorded by several adjacent electrodes. Oversampling (cross-talk) is considered to be a more serious problem than undersampling (Wheeler and Nowak, 1986;Stett et al, 2003;Morin et al, 2005). Despite the widespread utilization of microelectrode arrays, theoretical descriptions of the tissue-electrode interface are deficient because they contain too many unknown variables (e.g., signal intensity, distance from electrical source to recording electrodes, etc.…”

“…; Stett et al, 2003;Morin et al, 2005). We relied on experimental results performed with comparable microelectrode arrays in cerebellar and hippocampal slice preparations in vitro (Wheeler and Nowak, 1986;Egert et al, 2002). Single cell activity (ca.…”

Spatiotemporal dynamics of high-K+-induced epileptiform discharges in hippocampal slice and the effects of valproate

“…The application of MEA has greatly facilitated studies of the spatiotemporal properties of local networks in epileptiform discharges [12,16,[18][19][20] . Similar to the signals recorded with traditional electrodes [10,15] , epileptiform discharges are recorded as MUA characterized by a rapid series of action potentials superimposed on an FP (Fig.…”

Microelectrode array-based system for neuropharmacological applications with cortical neurons cultured in vitro

“…Preferably a sensitive extracellular multi-electrode system for signal detection should be employed to better capture the dynamics of these systems, which use distributed codes to store and process information (Deadwyler and Hampson, 1995;Buzsaki, 2004). Providing a means to non-destructively stimulate and record from many individual neurons simultaneously, microelectrode arrays (MEAs), with multiple extracellular microelectrodes built into the substrate, enable the studies related to spatio-temporal patterns of electrical activity in dissociated neuronal cultures or brain slices, such as formation of neuronal network, synaptic modification, regulation of network activity patterns, pharmacological evaluation and biological rhythms (Gross et al, 1977;Pine, 1980;Wheeler and Novak, 1986;Kamioka et al, 1996;Liu et al, 1997;Oka et al, 1999;Darbon et al, 2002;Marom and Shahaf, 2002;Egert et al, 2002;Martinoia et al, 2005;Arnold et al, 2005;Wagenaar et al, 2005).…”