Current State and Prospects of DNA Barcoding and DNA Fingerprinting in the Analysis of the Quality of Plant Raw Materials and Plant-Derived Drugs

Zhokhova, E. V.; Rodionov, A. V.; Повыдыш, М. Н.; Goncharov, M. Yu.; Protasova, Ya. A.; Yakovlev, G. P.

doi:10.1134/s2079086419040030

Cited by 7 publications

(9 citation statements)

References 92 publications

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“…Next-Generation Sequencing (NGS) technologies have been applied to herbal drug authentication using genome skimming or amplicon metabaroding approaches [ 1 , 8 , 87 , 88 , 89 , 90 , 91 , 92 ], including H. perforatum commercial products [ 16 ]. The quality of a herbal product is determined by the correct identity of its ingredients and its purity, measured by % contamination by a range of inorganic and biological contaminants.…”

Section: Discussionmentioning

confidence: 99%

Applied Barcoding: The Practicalities of DNA Testing for Herbals

Howard

Lockie-Williams

Slater

2020

Plants

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DNA barcoding is a widely accepted technique for the identification of plant materials, and its application to the authentication of commercial medicinal plants has attracted significant attention. The incorporation of DNA-based technologies into the quality testing protocols of international pharmacopoeias represents a step-change in status, requiring the establishment of standardized, reliable and reproducible methods. The process by which this can be achieved for any herbal medicine is described, using Hypericum perforatum L. (St John’s Wort) and potential adulterant Hypericum species as a case study. A range of practical issues are considered including quality control of DNA sequences from public repositories and the construction of individual curated databases, choice of DNA barcode region(s) and the identification of informative polymorphic nucleotide sequences. A decision tree informs the structure of the manuscript and provides a template to guide the development of future DNA barcode tests for herbals.

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Section: Discussionmentioning

confidence: 99%

Applied Barcoding: The Practicalities of DNA Testing for Herbals

Howard

Lockie-Williams

Slater

2020

Plants

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“…Chemical fingerprints can also be obtained from MS spectra which readily provide information on the presence of certain metabolites or elements within the HM samples based on their masses ( Yang and Deng, 2016 ). Besides chemical fingerprints, molecular fingerprints from the plant DNA can serve as a great authentication and standardization tool ( Zhokhova et al, 2019 ). Some advantages and disadvantages of these different methods in combination with chemometrics are shown in Table 1 .…”

Section: Applications Of Fingerprinting Methodsmentioning

confidence: 99%

“…The Consortium for the Barcode for Life (CBOL) proposed 7 plastid DNA regions for plant DNA barcoding including atp F -atp H , rbc L , rpo B , rpo C1 , mat K , psb K -psb I , and trn H -psb A ( CBOL-Plant-Working-Group et al, 2009 ) . Many other barcodes have been reported in the literature and the choice of barcodes has been discussed in several review articles ( Hollingsworth et al, 2011 ; Li et al, 2015 ; Zhokhova et al, 2019 ). Typically, these barcodes were evaluated based on their discriminating abilities.…”

Section: Applications Of Fingerprinting Methodsmentioning

confidence: 99%

Advances in Fingerprint Analysis for Standardization and Quality Control of Herbal Medicines

2022

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Herbal drugs or herbal medicines (HMs) have a long-standing history as natural remedies for preventing and curing diseases. HMs have garnered greater interest during the past decades due to their broad, synergistic actions on the physiological systems and relatively lower incidence of adverse events, compared to synthetic drugs. However, assuring reproducible quality, efficacy, and safety from herbal drugs remains a challenging task. HMs typically consist of many constituents whose presence and quantity may vary among different sources of materials. Fingerprint analysis has emerged as a very useful technique to assess the quality of herbal drug materials and formulations for establishing standardized herbal products. Rather than using a single or two marker(s), fingerprinting techniques take great consideration of the complexity of herbal drugs by evaluating the whole chemical profile and extracting a common pattern to be set as a criterion for assessing the individual material or formulation. In this review, we described and assessed various fingerprinting techniques reported to date, which are applicable to the standardization and quality control of HMs. We also evaluated the application of multivariate data analysis or chemometrics in assisting the analysis of the complex datasets from the determination of HMs. To ensure that these methods yield reliable results, we reviewed the validation status of the methods and provided perspectives on those. Finally, we concluded by highlighting major accomplishments and presenting a gap analysis between the existing techniques and what is needed to continue moving forward.

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“…For practical needs, the first half of the gene with a length of approximately 650 base pairs (bp) is conventionally used as a barcode. Other MMs or barcodes are more suitable for plants or fungi [9,10].…”

Section: Introductionmentioning

confidence: 99%

“…and forensic medicine in particular (exclusion of specimens from suspects in criminalistics). The scope of MM application includes also the monitoring of genetic safety to assess risks of the use of recombinant DNA and genetically modified organisms (cells) in the food and medical industries [10]. A few other applications of MMs, particularly in fishery/aquaculture, were reviewed separately [24,26].…”

Section: Introductionmentioning

confidence: 99%

Some Examples of the Use of Molecular Markers for Needs of Basic Biology and Modern Society

Kartavtsev

2021

Animals

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Application of molecular genetic markers appeared to be very fruitful in achieving many goals, including (i) proving the theoretic basements of general biology and (ii) assessment of worldwide biodiversity. Both are provided in the present meta-analysis and a review as the main signal. One of the basic current challenges in modern biology in the face of new demands in the 21st century is the validation of its paradigms such as the synthetic theory of evolution (STE) and biological species concept (BSC). Another of most valuable goals is the biodiversity assessment for a variety of social needs including free web-based information resources about any living being, renovation of museum collections, nature conservation that recognized as a global project, iBOL, as well as resolving global trading problems such as false labeling of species specimens used as food, drug components, entertainment, etc. The main issues of the review are focused on animals and combine four items. (1) A combination of nDNA and mtDNA markers best suits the identification of hybrids and estimation of genetic introgression. (2) The available facts on nDNA and mtDNA diversity seemingly make introgression among many taxa obvious, although it is evident, that introgression may be quite restricted or asymmetric, thus, leaving at least the “source” taxon (taxa) intact. (3) If we consider sexually reproducing species in marine and terrestrial realms introgressed, as it is still evident in many cases, then we should recognize that the BSC, in view of the complete lack of gene flow among species, is inadequate because many zoological species are not biological ones yet. However, vast modern molecular data have proven that sooner or later they definitely become biological species. (4) An investigation into the fish taxa divergence using the BOLD database shows that most gene trees are basically monophyletic and interspecies reticulations are quite rare.

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Current State and Prospects of DNA Barcoding and DNA Fingerprinting in the Analysis of the Quality of Plant Raw Materials and Plant-Derived Drugs

Cited by 7 publications

References 92 publications

Applied Barcoding: The Practicalities of DNA Testing for Herbals

Applied Barcoding: The Practicalities of DNA Testing for Herbals

Advances in Fingerprint Analysis for Standardization and Quality Control of Herbal Medicines

Some Examples of the Use of Molecular Markers for Needs of Basic Biology and Modern Society

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