Current Status and Future Perspective on Molecular Imaging and Treatment of Neuroblastoma

Feng, Ling; Li, Siqi; Wang, Chaoran; Yang, Jigang

doi:10.1053/j.semnuclmed.2022.12.004

Cited by 19 publications

(6 citation statements)

References 107 publications

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“…However, it is expensive and has the disadvantages of false-positive and false-negative results. Bone marrow aspiration and biopsy are the gold standard for diagnosing bone marrow metastasis and neuroblastoma recurrence and are also the recommended clinical diagnostic basis [54][55][56]. In this paper, an immunosensor was designed for GD2, which is an important detection target of neuroblastoma, and the corresponding GD2 antibody was modified on the sensor surface according to the specific binding of antigen and antibody in order to improve the detection of GD2 molecules on the surface of neuroblastoma.…”

Section: Discussionmentioning

confidence: 99%

Functionalized GD2 Electrochemical Immunosensor to Diagnose Minimum Residual Disease of Bone Marrow in Neuroblastoma Effectively

Chen,

Hu,

et al. 2023

Biosensors

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Neuroblastoma (NB) is known as the “king of childhood tumors” due to its highly metastatic, recurrence-prone, and difficult-to-treat characteristics. International Neuroblastoma Risk Grading Group (INRG) has recommended GD2, a disialoganglioside expressed on neuroectodermal tumor cells, as the target for detecting minimal residual disease in bone marrow metastases of high-risk neuroblastoma in children. Therefore, accurately identifying GD2-positive cells is crucial for diagnosing children with high-risk NB. Here, we designed a graphene/AuNP/GD2 Ab-functionalized electrochemical biosensor for GD2 detection. A three-electrode system was processed using a screen-printed technique with a working electrode of indium tin oxide, a counter electrode of carbon, and a reference electrode of silver/silver chloride. Graphene/AuNPs were modified on the indium tin oxide electrode using chronoamperometric scans, and then, the GD2 antibody was modified on the biosensor by electrostatic adsorption to achieve sensitive and specific detection of GD2-positive cells in bone marrow fluid. The results showed that a graphene/AuNP/GD2 Ab-functionalized electrochemical biosensor achieved GD2-positive cell detection in the range of 102 cells/mL~105 cells/mL by differential pulse voltammetry. Bone marrow fluid samples from 12 children with high-risk NB were retained for testing on our biosensor and showed 100% compliance with the clinical application of the gold-standard immunocytochemical staining technique for detecting GD2-positive cells qualitatively. The GD2-based electrochemical assay can accurately detect children with high-risk NB, providing a rapidly quantitative basis for clinical diagnosis and treatment.

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Section: Discussionmentioning

confidence: 99%

Functionalized GD2 Electrochemical Immunosensor to Diagnose Minimum Residual Disease of Bone Marrow in Neuroblastoma Effectively

Chen,

Hu,

et al. 2023

Biosensors

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“…The management of neuroblastoma is challenging for cancer specialists [ 2 ]. Conventional treatment of neuroblastoma includes several options (i.e., surgery, chemotherapy, radiotherapy, stem cell transplantation, and immunotherapy) [ [3] , [4] , [5] , [6] ]. Among all, chemotherapy, especially the combination drug therapy, was noted to be a treatment choice for patients whose extensive tumor resection is not recommended (i.e., immediate- and high-risk stages) [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of barakol from Cassia siamea on neuroblastoma SH-SY5Y cell line: A potential combined therapy with doxorubicin

Wongsawatkul,

Buachan,

Jaisin

et al. 2024

Heliyon

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“…The tumor usually occurs in young children, about 17 months, and accounts for 15% of all pediatric cancers. The disease often undergoes spontaneous regression in patients younger than one-year-old, even when the condition is metastatic (Feng et al, 2023;Richard et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Effect of P-glycoprotein Inhibition on Temozolomide Chemosensitivity on the Neuroblastoma Cell Line Sh-sy5y

Karademir

2023

Preprint

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Neuroblastoma is one of the most common and deadly childhood solid tumors. P-glycoprotein (P-gp) pump plays a role in developing resistance to many chemotherapeutic agents. The high expression of P-gp is associated with poor prognosis in drug resistance and neuroblastoma treatment. We aimed to evaluate the anticancer effect of Temozolomide (TMZ) on the SH-SY5Y human neuroblastoma cell line in the presence of P-gp inhibitor Verapamil (VER). In the present study, the antiproliferative effect of TMZ on SH-SY5Y cells alone and in combination with VER was evaluated using a colorimetric XTT viability test. SH-SY5Y cells were seeded to 96 well plates at 10,000 cells/well. TMZ (100 µM-10 mM) and VER (0.25-10 µg/ml) were applied to the cells alone first, then XTT measurements were performed after 24 and 48 hours. In the study's second phase, VER was applied to the cells at the fixed concentration of 2.5 µg/ml to block P-gp pumps, then increasing concentrations of TMZ were applied to the cells in the presence of VER. The current study showed that in addition to cytotoxic effects, VER + TMZ administration was found to accelerate apoptosis in SH-SY5Y cells compared to TMZ utilization alone. The wound healing assay demonstrated that VER + TMZ combination also inhibited cell migration. In light of this evidence, combining TMZ with VER in the neuroblastoma cell line may inhibit migration and cell proliferation via the apoptosis pathway and produce a substantial anticancer effect.

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Current Status and Future Perspective on Molecular Imaging and Treatment of Neuroblastoma

Cited by 19 publications

References 107 publications

Functionalized GD2 Electrochemical Immunosensor to Diagnose Minimum Residual Disease of Bone Marrow in Neuroblastoma Effectively

Functionalized GD2 Electrochemical Immunosensor to Diagnose Minimum Residual Disease of Bone Marrow in Neuroblastoma Effectively

Effects of barakol from Cassia siamea on neuroblastoma SH-SY5Y cell line: A potential combined therapy with doxorubicin

Effect of P-glycoprotein Inhibition on Temozolomide Chemosensitivity on the Neuroblastoma Cell Line Sh-sy5y

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