Current Status of the Development of Blood-Based Point-of-Care Microdevices

Laxmi, Vijai; Tripathi, Siddhartha; Agrawal, Amit

doi:10.1007/978-981-15-5712-5_8

Cited by 13 publications

(12 citation statements)

References 138 publications

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“…32 Despite availability of platelet separation and plasma separation microdevices, only a few facilitate enrichment of platelets in the plasma sample. Our survey 8,33 reveals that of late, microfluidics-based methods are gaining attention. The current work on the development of platelet enrichment microdevice is therefore topical.…”

Section: Introductionmentioning

confidence: 99%

Design Evolution and Performance Study of a Reliable Platelet-Rich Plasma Microdevice

Laxmi

Joshi

Agrawal

2020

Ind. Eng. Chem. Res.

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Separation and enrichment of platelets from whole blood find numerous applications for biomedical research, dermatology, and transfusion purposes. Therefore, there is a great value in selectively isolating and enriching platelets in plasma from whole blood. The conventional method of centrifugation involves several manual steps and cannot be integrated with point-of-care devices.Here, we present an evolved passive microdevice for the enhanced fold enrichment and separation efficiency of platelets in a platelet-enriched plasma sample. The design steps for arriving at the final design are also presented, as learnings from these early designs helped in evolution of the final microdevice; these early designs can also be used as a reference for further studies. The final proposed microdevice is easy to fabricate, requires less hardware and pumping power than available microdevices, and separates high-quality platelets from a whole blood sample. Our microdevice is able to achieve ∼15-fold platelet enrichment. Several biological tests attest to the high quality of platelets obtained from the microdevice. The performance of the developed microdevice is superior to those of few available microdevices. Our work suggests that passive microdevices can attain or even surpass the performance of active microdevices.

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Section: Introductionmentioning

confidence: 99%

Design Evolution and Performance Study of a Reliable Platelet-Rich Plasma Microdevice

Laxmi

Joshi

Agrawal

2020

Ind. Eng. Chem. Res.

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“…Much progress has been made in developing mobile blood devices, nevertheless, current mobile blood devices are limited to supply single or partial blood indicators and remain unfeasible for a full FWD, resulting in incomplete diagnosis information and limited diagnosis functions 3,45,46 . Therefore, satisfying mobile blood devices that supply enough blood indicators are still lacking.…”

Section: Introductionmentioning

confidence: 99%

Multiparameter mobile blood analyzer for complete blood count utilizing contrast-enhanced defocusing imaging

Chen

et al. 2022

Preprint

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Blood analysis, though complete blood count (CBC), is the most basic medical test for disease diagnosis and health monitoring. However, the need for bulky and expensive laboratory facilities and skilled technicians limits the universal medical practices based on blood analysis outside of well-equipped laboratory environments. Here, we proposed a multiparameter mobile blood analyzer utilizing label-free contrast-enhanced defocusing imaging (CEDI) with the assistance of machine vision for instant and on-site blood analysis. The analyzer using CEDI obtains both the blood cell morphological characteristics and hemoglobin spectrophotometric information for simultaneous five-part white blood cell differential count, red blood cell count, and hemoglobin quantification without the need for sample staining. In addition, we have designed a miniature microscope with a sub-micron spatial resolution of (~0.98 μm), which is quite small, lightweight, and cost-effective for blood imaging. We have shown that our assay can analyze a sample within 10 minutes, using just a drop of fingertip blood (~7 μl) and measurements (30 samples) from the analyzer have strong correlations with clinical reference values (significant level: P < 0.0001). The proposed mobile blood analyzer can help provide universal access to blood analysis and has great potential for integrated surveillance of various epidemic diseases, including coronavirus infection, invermination, and anemia, especially in low-and middle-income countries.

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“…Primarily in immunology, antibodies are used to label cell surface markers, however, their tendency to bind to certain cell populations is well known and therefore it is important to note that these might interfere with immunophenotyping, even when compared to matched isotype controls in flow cytomettry [19] and immunohistochemistry as well [20].However, single-cell-based approaches are already available for immunology as well [21]. Through micromanipulation, single cells can be isolated in microfluidic systems, for example, where various biosensors can be used to detect cells, their activation, or secreted proteins [22][23][24]. Label-free biosensor approaches provide further advantages since the activation of the cells can be measured in a kinetic manner; moreover, these methods also have the potential to study cells in a nondestructive way and thus enable downstream analysis and further processing of the cells with other techniques of interest.…”

Section: Introductionmentioning

confidence: 99%

Functional blood cell analysis by label-free biosensors and single-cell technologies

Szittner

Péter

Kurunczi

et al. 2022

Advances in Colloid and Interface Science

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Current Status of the Development of Blood-Based Point-of-Care Microdevices

Cited by 13 publications

References 138 publications

Design Evolution and Performance Study of a Reliable Platelet-Rich Plasma Microdevice

Design Evolution and Performance Study of a Reliable Platelet-Rich Plasma Microdevice

Multiparameter mobile blood analyzer for complete blood count utilizing contrast-enhanced defocusing imaging

Functional blood cell analysis by label-free biosensors and single-cell technologies

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