Current status of the immunology of blood and tissue protozoa. I. Leishmania

Zuckerman, Avivah

doi:10.1016/0014-4894(75)90123-x

Cited by 83 publications

(13 citation statements)

References 65 publications

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“…This type of experiment requires repeating in the L. ^m/j/ta/mouse system. although no effect (on lesion development) of serum transfers between genetically resistant donors and susceptible (BALB/c) recipients has yet been demonstrated with the parasite isolate used in the present study (Mitchell el ai, 1980;Zuckerman. 1975).…”

Section: Discussionmentioning

confidence: 60%

“…With regard to humoral mechanisms of immunity, no association has been found between anti-leishmanial antibody titres and the course ofthe disease (e.g. Radwanski et al, 1974) despite clear demonstrations of circulating antibodies in cutaneous leishmaniasis by numerous workers (Preston el al., 1972;Behforouz, Rezai and Gettner, 1976; other literature cited in Poulter, 1980;Zuckerman, 1975). However, eareful quantitative studies by Poulter (1980) have suggested that antibodies to L. enriellii in the guinea-pig may have some protective activity.…”

Section: Introductionmentioning

confidence: 99%

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ANTIBODIES TO LEISHMANIA TROPICA PROMASTIGOTES DURING INFECTION IN MICE OF VARIOUS GENOTYPES

Olobo

Handman

Curtis

et al. 1980

Aust J Exp Biol Med

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Summary. After intradermal injection of 10^ promastigotes of a particular isolate of the intramacrophage protozoan parasite, Leishmania ircpica. the developmeni ol disease (cutaneous lesions) is much more severe in BALB/c than in three other mouse strains, C57BL/6,C3H/He and CBA/H. Using fixed promastigotes and ''M-labcl!cd protein A in a solid-phase radioimmunoassay (RIA), titres of antibody were shown to increase up to about day 50 of infection. However, titres were not markedly different in BALB/c mice compared with the other three resistant strains, although antibody levels were highest in sera from tbe diseased BALB/c mice at late time points. Using isotype-specific antisera in the RIA, and sera from the day 50 time point, the isotype distribution of antipromastigote antibodies was not noticeably diiTerent in sera from mice ofthe four genotypes with IgG, and IgG^^ (it IgG^h) antibodies predominating. It is concluded that differences in susceptibility to disease in this murine model of cutaneous leishmaniasis do not correlate with any decrease or increase in any particular antibody response to the promastigote.

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Section: Discussionmentioning

confidence: 60%

Section: Introductionmentioning

confidence: 99%

ANTIBODIES TO LEISHMANIA TROPICA PROMASTIGOTES DURING INFECTION IN MICE OF VARIOUS GENOTYPES

Olobo

Handman

Curtis

et al. 1980

Aust J Exp Biol Med

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“…Immunization with this molecule could induce a first level of immune attack by antibodies, which may prevent parasite attachment to macrophages. Parasites that are not internalized rapidly by macrophages die in the extracellular space in a few minutes (20). If, however, one parasite succeeds in gaining entrance into a macrophage, infection may occur.…”

Section: Discussionmentioning

confidence: 99%

Immunization with Leishmania receptor for macrophages protects mice against cutaneous leishmaniasis.

Handman¹,

Mitchell²

1985

Proc. Natl. Acad. Sci. U.S.A.

121

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The Leishmania major receptor for macrophages is a lipid-containing glycoconjugate that is recognized by the monoclonal antibody WIC-79.3. When L. major promastigotes were incubated with Fab fragments of prior to injection into genetically susceptible mice, their infectivity was decreased. Fab fragments from an irrelevant control antibody of the same class had no effect. The L. major glycolipid was purified from detergent-solubilized promastigotes by affinity chromatography on immobilized WIC-79

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“…Although CPs have been identified and studied for more than 30 years in Leishmania spp. (Zuckerman 1975), there is still limited information, especially in L. (V.) braziliensis, where enzymatic evidences of the CPs are scarce. Most of the CPs characterized in Leishmania spp.…”

Section: Discussionmentioning

confidence: 98%

Cysteine proteinases from promastigotes of Leishmania (Viannia) braziliensis

et al. 2009

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Leishmania (Viannia) braziliensis is the major causative agent of American tegumentary leishmaniasis, a disease that has a wide geographical distribution and is a severe public health problem. The cysteine proteinase B (CPB) from Leishmania spp. represents an important virulence factor. In this study, we characterized and localized cysteine proteinases in L. (V.) braziliensis promastigotes. By a combination of triton X-114 extraction, concanavalin A-affinity, and ion exchange chromatographies, we obtained an enriched fraction of hydrophobic proteins rich in mannose residues. This fraction contained two proteinases of 63 and 43 kDa, which were recognized by a CPB antiserum, and were partially sensitive to E-64 in enzymatic assays with the peptide Glu-Phe-Leu. In confocal microscopy, the CPB homologues localized in the peripheral region of the parasite. This data together with direct agglutination and flow cytometry assays suggest a surface localization of the CPB homologues. The incubation of intact promastigotes with phospholipase C reduced the number of CPB-positive cells, while anti-cross-reacting determinant and anti-CPB antisera recognized two polypeptides (63 and 43 kDa) derived from phospholipase C treatment, suggesting that some CPB isoforms may be glycosylphosphatidylinositol-anchored. Collectively, our results suggest the presence of CPB homologues in L. braziliensis surface and highlight the need for further studies on L. braziliensis cysteine proteinases, which require enrichment methods for enzymatic detection.

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Current status of the immunology of blood and tissue protozoa. I. Leishmania

Cited by 83 publications

References 65 publications

ANTIBODIES TO LEISHMANIA TROPICA PROMASTIGOTES DURING INFECTION IN MICE OF VARIOUS GENOTYPES

ANTIBODIES TO LEISHMANIA TROPICA PROMASTIGOTES DURING INFECTION IN MICE OF VARIOUS GENOTYPES

Immunization with Leishmania receptor for macrophages protects mice against cutaneous leishmaniasis.

Cysteine proteinases from promastigotes of Leishmania (Viannia) braziliensis

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