Current Status of Trace Metal Pollution in Soils Affected by Industrial Activities

Kabir, Ehsanul; Ray, Sharmila; Kim, Ki‐Hyun; Yoon, Hee Chang; Jeon, Eui-Chan; Kim, Yoon Shin; Cho, Yong Sung; Yun, Seong Taek; Brown, Richard

doi:10.1100/2012/916705

Cited by 126 publications

(61 citation statements)

References 76 publications

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“…On the other hand, excess iron may also interfere with components of the copper homeostasis and sensitize the bacteria to copper toxicity under anaerobic conditions (5). Since polluted water often contains different toxic metals (3,4), the interplay between copper and iron/iron-sulfur clusters in microorganisms will require further investigation.…”

Section: Discussionmentioning

confidence: 99%

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Anaerobic Copper Toxicity and Iron-Sulfur Cluster Biogenesis in Escherichia coli

Tan

Yang

Tang

et al. 2017

Appl Environ Microbiol

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While copper is an essential trace element in biology, pollution of groundwater from copper has become a threat to all living organisms. Cellular mechanisms underlying copper toxicity, however, are still not fully understood. Previous studies have shown that iron-sulfur proteins are among the primary targets of copper toxicity in Escherichia coli under aerobic conditions. Here, we report that, under anaerobic conditions, iron-sulfur proteins in E. coli cells are even more susceptible to copper in medium. Whereas addition of 0.2 mM copper(II) chloride to LB (Luria-Bertani) medium has very little or no effect on iron-sulfur proteins in wild-type E. coli cells under aerobic conditions, the same copper treatment largely inactivates iron-sulfur proteins by blocking iron-sulfur cluster biogenesis in the cells under anaerobic conditions. Importantly, proteins that do not have iron-sulfur clusters (e.g., fumarase C and cysteine desulfurase) in E. coli cells are not significantly affected by copper treatment under aerobic or anaerobic conditions, indicating that copper may specifically target iron-sulfur proteins in cells. Additional studies revealed that E. coli cells accumulate more intracellular copper under anaerobic conditions than under aerobic conditions and that the elevated copper content binds to the iron-sulfur cluster assembly proteins IscU and IscA, which effectively inhibits iron-sulfur cluster biogenesis. The results suggest that the copper-mediated inhibition of iron-sulfur proteins does not require oxygen and that iron-sulfur cluster biogenesis is the primary target of anaerobic copper toxicity in cells.IMPORTANCE Copper contamination in groundwater has become a threat to all living organisms. However, cellular mechanisms underlying copper toxicity have not been fully understood up to now. The work described here reveals that iron-sulfur proteins in Escherichia coli cells are much more susceptible to copper in medium under anaerobic conditions than they are under aerobic conditions. Under anaerobic conditions, E. coli cells accumulate excess intracellular copper, which specifically targets iron-sulfur proteins by blocking iron-sulfur cluster biogenesis. Since iron-sulfur proteins are involved in diverse and vital physiological processes, inhibition of ironsulfur cluster biogenesis by copper disrupts multiple cellular functions and ultimately inhibits cell growth. The results from this study illustrate a new interplay between intracellular copper toxicity and iron-sulfur cluster biogenesis in bacterial cells under anaerobic conditions.

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Section: Discussionmentioning

confidence: 99%

“…In U.S. groundwater, copper concentrations range from undetectable to approximately 1 mM, with most samples in the nanomolar range (3). In industrial and mining waste streams, micromolar to millimolar concentrations of copper have also been found (4). Copper at high concentrations in water has become a threat to all living organisms (5).…”

mentioning

confidence: 99%

Anaerobic Copper Toxicity and Iron-Sulfur Cluster Biogenesis in Escherichia coli

Tan

Yang

Tang

et al. 2017

Appl Environ Microbiol

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“…A survey of public testing data showed that copper levels in U.S. groundwater range from undetectable to approximately 1 mM, with most samples in the nanomolar range (9). Micro-to millimolar concentrations, which represent the high end of the range, are found particularly in industrial and mining waste streams (10,11). There are several mechanisms by which copper is toxic (6,12,13).…”

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confidence: 99%

Iron and Copper Act Synergistically To Delay Anaerobic Growth of Bacteria

Bird

Coleman

Newman

2013

Appl Environ Microbiol

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bTransition metals are known to cause toxic effects through their interaction with oxygen, but toxicity under anoxic conditions is poorly understood. Here we investigated the effects of iron (Fe) and copper (Cu) on the anaerobic growth and gene expression of the purple phototrophic bacterium Rhodopseudomonas palustris TIE-1. We found that Fe(II) and Cu(II) act synergistically to delay anaerobic growth at environmentally relevant metal concentrations. Cu(I) and Cu(II) had similar effects both alone and in the presence of ascorbate, a Cu(II) reductant, indicating that reduction of Cu(II) to Cu(I) by Fe(II) is not sufficient to explain the growth inhibition. Addition of Cu(II) increased the toxicity of Co(II) and Ni(II); in contrast, Ni(II) toxicity was diminished in the presence of Fe(II). The synergistic anaerobic toxicity of Fe(II) and Cu(II) was also observed for Escherichia coli MG1655, Shewanella oneidensis MR-1, and Rhodobacter capsulatus SB1003. Gene expression analyses for R. palustris identified three regulatory genes that respond to Cu(II) and not to Fe(II): homologs of cueR and cusR, two known proteobacterial copper homeostasis regulators, and csoR, a copper regulator recently identified in Mycobacterium tuberculosis. Two P-type ATPase efflux pumps, along with an F o F 1 ATP synthase, were also upregulated by Cu(II) but not by Fe(II). An Escherichia coli mutant deficient in copA, cus, and cueO showed a smaller synergistic effect, indicating that iron might interfere with one or more of the copper homeostasis systems. Our results suggest that interactive effects of transition metals on microbial physiology may be widespread under anoxic conditions, although the molecular mechanisms remain to be more fully elucidated.

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“…68 Bhaluka industrial area in Mymensingh was infested with various textiles and garment industries. Textile industries soils contain high levels of lead than in textile effluents in Bangladesh [84]. [85].…”

Section: Heavy Metal Pollution Of City Areamentioning

confidence: 99%

A Critical Review of Heavy Metal Pollution and Its Effects in Bangladesh

Bhuyan¹

2017

SJEE

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Abstract:Heavy metal, one of the most hazardous pollutant that can pose serious threat to human and environment. The concentrations of metals are increasing at an alarming rate due to boost of unplanned industrialization and urbanization. Though some metals are playing crucial role as micronutrients but the excessive amount exert negative impact at great extent. The existence of toxic heavy metals in the air, water and sediment can cause severe problems to all organisms because of their long persistence nature and bioaccumulation in the food chain. The present study reviews the various area of Bangladesh to make information on the sources of metal pollution, dissemination of metals in the environment and their possible effects on atmosphere, water, sediment, fishes and vegetables (plants).

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Current Status of Trace Metal Pollution in Soils Affected by Industrial Activities

Cited by 126 publications

References 76 publications

Anaerobic Copper Toxicity and Iron-Sulfur Cluster Biogenesis in Escherichia coli

Anaerobic Copper Toxicity and Iron-Sulfur Cluster Biogenesis in Escherichia coli

Iron and Copper Act Synergistically To Delay Anaerobic Growth of Bacteria

A Critical Review of Heavy Metal Pollution and Its Effects in Bangladesh

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