Current stem cells technologies used in medicine

Müller, Maria; Czarnecka, Julia; Brzeziński, Michał; Prus, Jakub; Kulak, Bartosz; Hołubowski, Andrzej; Stasiak, Mateusz; Borowiec, Blanka; Bryl, Rut; Moncrieff, Lisa; Dyszkiewicz-Konwińska, Marta

doi:10.2478/acb-2020-0016

Cited by 4 publications

(3 citation statements)

References 106 publications

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“…Adult progenitor cells, such as those derived from the bone marrow, have been effectively used in treatment of hematopoiesis-associated diseases (e.g., leukemia) since the 1970s [9,10]. Adult stem cells present a range of advantages over their pluripotent counterparts, such as their ready availability in the adult organism, a narrowed range of differentiation lineages, limiting the possibility of malignant development after implantation, and the possibility of collection without major surgical procedures [11][12][13][14][15]. Furthermore, a significant amount of recent research, especially regarding the treatment of bone-and cartilage-related diseases, focuses on adipose-derived stem cells (ASCs) [1,16,17].…”

Section: Introductionmentioning

confidence: 99%

Expression Profile of New Marker Genes Involved in Differentiation of Canine Adipose-Derived Stem Cells into Osteoblasts

Jankowski

Kaczmarek

Wąsiatycz

et al. 2021

IJMS

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Next-generation sequencing (RNAseq) analysis of gene expression changes during the long-term in vitro culture and osteogenic differentiation of ASCs remains to be important, as the analysis provides important clues toward employing stem cells as a therapeutic intervention. In this study, the cells were isolated from adipose tissue obtained during routine surgical procedures and subjected to 14-day in vitro culture and differentiation. The mRNA transcript levels were evaluated using the Illumina platform, resulting in the detection of 19,856 gene transcripts. The most differentially expressed genes (fold change >|2|, adjusted p value < 0.05), between day 1, day 14 and differentiated cell cultures were extracted and subjected to bioinformatical analysis based on the R programming language. The results of this study provide molecular insight into the processes that occur during long-term in vitro culture and osteogenic differentiation of ASCs, allowing the re-evaluation of the roles of some genes in MSC progression towards a range of lineages. The results improve the knowledge of the molecular mechanisms associated with long-term in vitro culture and differentiation of ASCs, as well as providing a point of reference for potential in vivo and clinical studies regarding these cells’ application in regenerative medicine.

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Section: Introductionmentioning

confidence: 99%

Expression Profile of New Marker Genes Involved in Differentiation of Canine Adipose-Derived Stem Cells into Osteoblasts

Jankowski

Kaczmarek

Wąsiatycz

et al. 2021

IJMS

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“…Perinatal stem cell clinical trials must be approved by the local bioethical commissions and be conducted in line with the EU clinical trials registration law [ 162 , 163 ]. Cells of fetal origin, also known as perinatal stem cells, are derived from extraembryonic structures, such as the placenta, umbilical cord, and amniotic fluid [ 164 , 165 ]. Perinatal tissues are also widely used as a reservoir of hematopoietic progenitor cells obtained from the umbilical cord blood [ 166 ].…”

Section: Mesenchymal Stem/stromal Cells Derived From Human and Animal Perinatal Tissuesmentioning

confidence: 99%

Mesenchymal Stem/Stromal Cells Derived from Human and Animal Perinatal Tissues—Origins, Characteristics, Signaling Pathways, and Clinical Trials

Kulus

Sibiak

Stefańska

et al. 2021

Cells

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Mesenchymal stem/stromal cells (MSCs) are currently one of the most extensively researched fields due to their promising opportunity for use in regenerative medicine. There are many sources of MSCs, of which cells of perinatal origin appear to be an invaluable pool. Compared to embryonic stem cells, they are devoid of ethical conflicts because they are derived from tissues surrounding the fetus and can be safely recovered from medical waste after delivery. Additionally, perinatal MSCs exhibit better self-renewal and differentiation properties than those derived from adult tissues. It is important to consider the anatomy of perinatal tissues and the general description of MSCs, including their isolation, differentiation, and characterization of different types of perinatal MSCs from both animals and humans (placenta, umbilical cord, amniotic fluid). Ultimately, signaling pathways are essential to consider regarding the clinical applications of MSCs. It is important to consider the origin of these cells, referring to the anatomical structure of the organs of origin, when describing the general and specific characteristics of the different types of MSCs as well as the pathways involved in differentiation.

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“…Hence, adult stem cells, such as bone marrow stem cells (BMSCs) are the source used for most of the currently developed therapeutic approaches [ 10 , 11 ]. Moreover, there have recently been some significant developments regarding another source of adult mesenchymal stem cells (MSCs), namely adipose-derived stem cells (ASCs) [ 12 , 13 , 14 , 15 , 16 ]. These stem cells can be freely obtained from the adipose tissue of adult mammals, and exhibit very similar characteristics to BMSCs, while being much easier to collect without almost any patient burden.…”

Section: Introductionmentioning

confidence: 99%

Expression Profile of New Gene Markers Involved in Differentiation of Canine Adipose-Derived Stem Cells into Chondrocytes

Jankowski

Kaczmarek

Wąsiatycz

et al. 2022

Genes

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The interest in stem cell research continuously increased over the last decades, becoming one of the most important trends in the 21st century medicine. Stem cell-based therapies have a potential to become a solution for a range of currently untreatable diseases, such as spinal cord injuries, type I diabetes, Parkinson’s disease, heart disease, stroke, and osteoarthritis. Hence, this study, based on canine material, aims to investigate the molecular basis of adipose-derived stem cell (ASC) differentiation into chondrocytes, to serve as a transcriptomic reference for further research aiming to introduce ASC into treatment of bone and cartilage related diseases, such as osteoarthritis in veterinary medicine. Adipose tissue samples were harvested from a canine specimen subjected to a routine ovariohysterecromy procedure at an associated veterinary clinic. The material was treated for ASC isolation and chondrogenic differentiation. RNA samples were isolated at day 1 of culture, day 30 of culture in unsupplemented culture media, and day 30 of culture in chondrogenic differentiation media. The resulting RNA was analyzed using RNAseq assays, with the results validated by RT-qPCR. Between differentiated chondrocytes, early and late cultures, most up- and down-regulated genes in each comparison were selected for further analysis., there are several genes (e.g., MMP12, MPEG1, CHI3L1, and CD36) that could be identified as new markers of chondrogenesis and the influence of long-term culture conditions on ASCs. The results of the study prove the usefulness of the in vitro culture model, providing further molecular insight into the processes associated with ASC culture and differentiation. Furthermore, the knowledge obtained could be used as a molecular reference for future in vivo and clinical studies.

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Current stem cells technologies used in medicine

Cited by 4 publications

References 106 publications

Expression Profile of New Marker Genes Involved in Differentiation of Canine Adipose-Derived Stem Cells into Osteoblasts

Expression Profile of New Marker Genes Involved in Differentiation of Canine Adipose-Derived Stem Cells into Osteoblasts

Mesenchymal Stem/Stromal Cells Derived from Human and Animal Perinatal Tissues—Origins, Characteristics, Signaling Pathways, and Clinical Trials

Expression Profile of New Gene Markers Involved in Differentiation of Canine Adipose-Derived Stem Cells into Chondrocytes

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