Current strategies for SARS-CoV-2 molecular detection

Xie, Lei; Li, Junlin; Ai, Ying; He, Hong; Chen, Xiuyun; Yin, Mingyu; Li, Wanxi; Huang, Wenguan; Luo, Minyi; He, Jinyang

doi:10.1039/d2ay01313d

Anal. Methods

2022

DOI: 10.1039/d2ay01313d

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Current strategies for SARS-CoV-2 molecular detection

Lei Xie

Junlin Li

Ying Ai

et al.

Abstract: Molecular detection of the SARS-CoV-2 is extremely important for the discovery and prevention of the pandemic dissemination. Because the SARS-CoV-2 is not always present in samples that can be collected,...

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2024

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Surface Plasmon Resonance Immunosensor for Direct Detection of Antibodies against SARS-CoV-2 Nucleocapsid Protein

Lisyte,

Kausaite-Minkstimiene,

Brasiunas

et al. 2024

IJMS

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The strong immunogenicity of the SARS-CoV-2 nucleocapsid protein is widely recognized, and the detection of specific antibodies is critical for COVID-19 diagnostics in patients. This research proposed direct, label-free, and sensitive detection of antibodies against the SARS-CoV-2 nucleocapsid protein (anti-SCoV2-rN). Recombinant SARS-CoV-2 nucleocapsid protein (SCoV2-rN) was immobilized by carbodiimide chemistry on an SPR sensor chip coated with a self-assembled monolayer of 11-mercaptoundecanoic acid. When immobilized under optimal conditions, a SCoV2-rN surface mass concentration of 3.61 ± 0.52 ng/mm2 was achieved, maximizing the effectiveness of the immunosensor for the anti-SCoV2-rN determination. The calculated KD value of 6.49 × 10−8 ± 5.3 × 10−9 M confirmed the good affinity of the used monoclonal anti-SCoV2-rN antibodies. The linear range of the developed immunosensor was from 0.5 to 50 nM of anti-SCoV2-rN, where the limit of detection and the limit of quantification values were 0.057 and 0.19 nM, respectively. The immunosensor exhibited good reproducibility and specificity. In addition, the developed immunosensor is suitable for multiple anti-SCoV2-rN antibody detections.

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Surface Plasmon Resonance Immunosensor for Direct Detection of Antibodies against SARS-CoV-2 Nucleocapsid Protein

Lisyte,

Kausaite-Minkstimiene,

Brasiunas

et al. 2024

IJMS

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show abstract

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Current strategies for SARS-CoV-2 molecular detection

Abstract: Molecular detection of the SARS-CoV-2 is extremely important for the discovery and prevention of the pandemic dissemination. Because the SARS-CoV-2 is not always present in samples that can be collected,...

Cited by 1 publication

References 136 publications

Surface Plasmon Resonance Immunosensor for Direct Detection of Antibodies against SARS-CoV-2 Nucleocapsid Protein

Surface Plasmon Resonance Immunosensor for Direct Detection of Antibodies against SARS-CoV-2 Nucleocapsid Protein

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